A5087531191- Axon Guidance and Neuronal Signaling
- Neurogenesis and neuroplasticity mechanisms
- Zebrafish Biomedical Research Applications
- Nerve injury and regeneration
- Angiogenesis and VEGF in Cancer
- Hippo pathway signaling and YAP/TAZ
- Neuroblastoma Research and Treatments
- Single-cell and spatial transcriptomics
- Congenital heart defects research
- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Neuroscience and Neuropharmacology Research
- Cellular Mechanics and Interactions
- Wnt/β-catenin signaling in development and cancer
- Apelin-related biomedical research
- Developmental Biology and Gene Regulation
- Photoreceptor and optogenetics research
- Cancer Research and Treatments
- Melanoma and MAPK Pathways
- Microtubule and mitosis dynamics
- Immunotherapy and Immune Responses
- Molecular Biology Techniques and Applications
- Hereditary Neurological Disorders
- Fungal and yeast genetics research
- Glycosylation and Glycoproteins Research
Université Claude Bernard Lyon 1
2014-2024
Centre National de la Recherche Scientifique
2014-2024
Inserm
2005-2024
Institut NeuroMyoGène
2016-2024
Communauté urbaine de Lyon
2024
Laboratoire de Génétique Cellulaire
1996-2019
Centre de Génétique Moléculaire
2005-2008
Institut de Biologie du Développement Marseille
2002-2006
Baylor University Medical Center
2003
University of Arizona
2003
The chemotropic guidance cue netrin-1 promotes neurite outgrowth through its receptor Deleted in Colorectal Cancer (DCC) via activation of Rac1. guanine nucleotide exchange factor (GEF) linking netrin-1/DCC to Rac1 has not yet been identified. Here, we show that the RhoGEF Trio mediates signaling. We found interacts with DCC mouse embryonic brains and netrin-1-induced brain is impaired absence Trio. Trio−/− cortical neurons fail extend neurites response netrin-1, while they are able respond...
Commissural axon guidance requires complex modulations of growth cone sensitivity to midline-derived cues, but underlying mechanisms in vertebrates remain largely unknown. By using combinations ex vivo and approaches, we uncovered a molecular pathway controlling the gain response midline repellent, Semaphorin3B (Sema3B). First, provide evidence that Semaphorin3B/Plexin-A1 signaling participates commissural projections at vertebrate ventral midline. Second, show that, precrossing stage,...
Neuromuscular junction (NMJ) formation requires the highly coordinated communication of several reciprocal signaling processes between motoneurons and their muscle targets. Identification early, spatially restricted cues in target recognition at NMJ is still poorly documented, especially mammals. Wnt one key pathways regulating synaptic connectivity. Here, we report that Wnt4 contributes to vertebrate vivo. Results from a microarray screen quantitative RT-PCR demonstrate expression regulated...
Reconstructing functional neuronal circuits is one major challenge of central nervous system repair. Through activation pro-growth signaling pathways, some neurons achieve long-distance axon regrowth. Yet, reconnection has hardly been obtained, as these regenerating axons fail to resume their initial trajectory and reinnervate proper target. Axon guidance considered be active only during development. Here, using the mouse visual system, we show that still in adult brain regenerative...
The structural basis of cortical columns are radially oriented axon collaterals that form precise connections between distinct layers. During development, these highly specified from the initial outgrowth collateral branches. Our previous work provided evidence for positional cues confined to individual layers induce and/or prevent formation in specific populations neurons. Here we demonstrated with<i>in situ</i> hybridization techniques mRNA Eph receptor tyrosine kinase EphA5 and one its...
Previously, we demonstrated that loss of SEMA3F, a secreted semaphorin encoded in 3p21.3, is associated with higher stages lung cancer and primary tumor cells studied anti-vascular endothelial growth factor (VEGF) SEMA3F antibodies. In vitro, inhibits cell spreading; this activity opposed by VEGF. These results suggest VEGF compete for binding to their common neuropilin receptor. the present report, investigated attractive/repulsive effects on migration when were grown threedimensional...
Class III semaphorins (SemaIIIs) are intercellular cues secreted by surrounding tissues to guide migrating cells and axons in the developing organism. This chemotropic activity is crucial for formation of nerves vasculature. Intriguingly, SemaIIIs also synthesized neurons during axon pathfinding, but their function as intrinsic remains unknown. We have explored role Sema3A expression motoneurons spinal nerve development. Loss- gain-of-function neural tube chick embryo were undertaken target...
Neural migration is a critical step during brain development that requires the interactions of cell-surface guidance receptors. Cancer cells often hijack these mechanisms to disseminate. Here, we reveal crystal structures Uncoordinated-5 receptor D (Unc5D) in complex with morphogen glypican-3 (GPC3), forming an octameric glycoprotein complex. In complex, four Unc5D molecules pack into antiparallel bundle, flanked by GPC3 molecules. Central glycan-glycan are formed N-linked glycans emanating...
During development, axons migrate long distances in responses to attractive or repulsive signals that are detected by their growth cones. One of these is mediated netrin-1, a diffusible laminin-related molecule both attracts and repels cones via interaction with its receptor DCC (deleted colorectal cancer). Here we show commissural neurons immortalized cells, partially associated cholesterol- sphingolipid-enriched membrane domains named lipid rafts. This localization rafts the palmitoylation...
Abstract Semaphorins are major chemorepellents for developing neuronal projections. Their persistent expression at adult stages suggests that they may contribute to the functioning of circuits. We investigated functional properties semaphorin3A (Sema3A) in hippocampal neurons, and report exogenous application this cue decreases efficacy synaptic transmission evoked CA1 region slices. In situ hybridization, imaging biochemical techniques showed Sema3A receptor component neuropilin‐1 is...
Accurate perception of guidance cues is crucial for cell and axon migration. During initial navigation in the spinal cord, commissural axons are kept insensitive to midline repellents. Upon crossing floor plate, they switch on responsiveness Slit Semaphorin repulsive signals thus propelled away prevented from back. Whether how different repellents control specific aspects this remain be elucidated. We set up a paradigm live-imaging super-resolution analysis PlexinA1, Neuropilin-2, Robo1/2...
The spatial orientation of cell divisions is fundamental for tissue architecture and homeostasis. Here we analysed neuroepithelial progenitors in the developing mouse spinal cord to determine whether extracellular signals orient mitotic spindle. We report that Semaphorin3B (Sema3B) released from floor plate nascent choroid plexus cerebrospinal fluid (CSF) controls progenitor division orientation. Delivery exogenous Sema3B neural after tube opening living embryos promotes planar their...
Abstract Embryonic malignant transformation is concomitant to organogenesis, often affecting multipotent and migratory progenitors. While lineage relationships between cells their physiological counterparts are extensively investigated, the contribution of exogenous embryonic signals not fully known. Neuroblastoma (NB) a childhood malignancy peripheral nervous system arising from trunk neural crest (NC) characterized by heterogeneous interconvertible tumor cell identities. Here, using...