A5069810890- Axon Guidance and Neuronal Signaling
- Neurogenesis and neuroplasticity mechanisms
- Hippo pathway signaling and YAP/TAZ
- Neuroblastoma Research and Treatments
- Nerve injury and regeneration
- Arsenic contamination and mitigation
- Glycosylation and Glycoproteins Research
- Developmental Biology and Gene Regulation
- Single-cell and spatial transcriptomics
- Zebrafish Biomedical Research Applications
- Immune cells in cancer
- Signaling Pathways in Disease
- Heavy metals in environment
- Microtubule and mitosis dynamics
- Caveolin-1 and cellular processes
- Heavy Metal Exposure and Toxicity
- Chromium effects and bioremediation
- Viral Infectious Diseases and Gene Expression in Insects
- Fungal and yeast genetics research
- Selenium in Biological Systems
- Ion Transport and Channel Regulation
- RNA Research and Splicing
- Nitrogen and Sulfur Effects on Brassica
- Plant Molecular Biology Research
- Cancer, Hypoxia, and Metabolism
Centre National de la Recherche Scientifique
2009-2024
Université Claude Bernard Lyon 1
2014-2024
Inserm
2017-2024
Institut NeuroMyoGène
2016-2022
Karolinska Institutet
2020
Laboratoire Physiologie Cellulaire & Végétale
2009
Université Joseph Fourier
2009
Institut de Recherches en Technologies et Sciences pour le Vivant
2009
Institute of Environmental Biology and Biotechnology
2008
CEA Cadarache
2008
Cadmium poses a significant threat to human health due its toxicity. In mammals and in bakers' yeast, cadmium is detoxified by ATP-binding cassette transporters after conjugation glutathione. fission phytochelatins constitute the co-substrate with for transporter SpHMT1. plants, detoxification mechanism similar one yeast supposed, but molecular nature of still lacking. To investigate further relationship between SpHMT1 co-substrate, we overexpressed Schizosaccharomyces pombe strain deleted...
Abstract Selenium-Binding Protein1 (SBP1) gene expression was studied in Arabidopsis (Arabidopsis thaliana) seedlings challenged with several stresses, including cadmium (Cd), selenium {selenate [Se(VI)] and selenite [Se(IV)]}, copper (Cu), zinc (Zn), hydrogen peroxide (H2O2) using transgenic lines expressing the luciferase (LUC) reporter under control of SBP1 promoter. In roots shoots SBP1∷LUC lines, LUC activity increased response to Cd, Se(VI), Cu, H2O2 but not Se(IV) or Zn. The pattern...
Neural migration is a critical step during brain development that requires the interactions of cell-surface guidance receptors. Cancer cells often hijack these mechanisms to disseminate. Here, we reveal crystal structures Uncoordinated-5 receptor D (Unc5D) in complex with morphogen glypican-3 (GPC3), forming an octameric glycoprotein complex. In complex, four Unc5D molecules pack into antiparallel bundle, flanked by GPC3 molecules. Central glycan-glycan are formed N-linked glycans emanating...
The sequential generation of layer-specific cortical neurons requires radial glia cells (RGCs) to precisely balance self-renewal and lineage commitment. While specific cell-cycle phases have been associated with these decisions, the mechanisms linking machinery cell-fate commitment remain obscure. Using single-cell RNA-sequencing, we find that strongest transcriptional signature defining multipotent RGCs is G2/M-phase, particularly CYCLIN-B1/2, while lineage-committed progenitors are...
The spatial orientation of cell divisions is fundamental for tissue architecture and homeostasis. Here we analysed neuroepithelial progenitors in the developing mouse spinal cord to determine whether extracellular signals orient mitotic spindle. We report that Semaphorin3B (Sema3B) released from floor plate nascent choroid plexus cerebrospinal fluid (CSF) controls progenitor division orientation. Delivery exogenous Sema3B neural after tube opening living embryos promotes planar their...
Abstract Embryonic malignant transformation is concomitant to organogenesis, often affecting multipotent and migratory progenitors. While lineage relationships between cells their physiological counterparts are extensively investigated, the contribution of exogenous embryonic signals not fully known. Neuroblastoma (NB) a childhood malignancy peripheral nervous system arising from trunk neural crest (NC) characterized by heterogeneous interconvertible tumor cell identities. Here, using...
During corticogenesis, dynamic regulation of apical adhesion is fundamental to generate correct numbers and cell identities. While radial glial cells (RGCs) maintain basal anchors, progenitors neurons detach settle at distal positions from the border. Whether diffusible signals delivered cerebrospinal fluid (CSF) contribute dynamics remains fully unknown. Secreted class 3 Semaphorins (Semas) trigger responses via Plexin-Neuropilin (Nrp) membrane receptor complexes. Here, we report that...
Despite their indisputable importance in neuroblastoma (NB) pathology, knowledge of the bases NB plasticity and heterogeneity remains incomplete. They may be rooted developmental trajectories lineage origin, sympatho-adrenal neural crest. We find that implanting human cells crest avian embryo allows recapitulating metastatic sequence until bone marrow involvement. Using deep single cell RNA sequencing, we characterize transcriptome states dynamics over time space, compare them to those fetal...
Membrane microdomains or “lipid rafts” have emerged as essential functional modules of the cell, critical for regulation growth factor receptor-mediated responses. Herein we describe dichotomy between caveolin-1 and caveolin-2, structural regulatory components microdomains, in modulating proliferation differentiation. Caveolin-2 potentiates while inhibits nerve (NGF) signaling subsequent cell does not appear to impair NGF receptor trafficking but elicits prolonged stronger activation MAPK...
The development of gene transfection technologies has greatly advanced our understanding life sciences. While use viral vectors clear efficacy, it requires specific expertise and biological containment conditions. Electroporation become an effective commonly used method for introducing DNA into neurons in intact brain tissue. present study describes the Neon® electroporation system to transfect genes dorsal root ganglia isolated from embryonic mouse Day 13.5 16. This cell type been...
Summary Neural migration is a critical step during brain development that requires the interactions of cell-surface guidance receptors. Cancer cells often hijack these mechanisms to disseminate. Here we reveal crystal structures Uncoordinated-5 receptor D (Unc5D) in complex with morphogen glypican-3 (GPC3), forming an octameric glycoprotein complex. In complex, four Unc5D molecules pack into antiparallel bundle, flanked by GPC3 molecules. Central glycan-glycan are formed N-linked glycans...