A5050819134- Cellular transport and secretion
- Microtubule and mitosis dynamics
- Protist diversity and phylogeny
- Mitochondrial Function and Pathology
- Photoreceptor and optogenetics research
- Photosynthetic Processes and Mechanisms
- Telomeres, Telomerase, and Senescence
- Genetics, Aging, and Longevity in Model Organisms
Uniformed Services University of the Health Sciences
2019-2024
Deficiency of the LIS1 protein causes lissencephaly, a brain developmental disorder. Although binds microtubule motor cytoplasmic dynein and has been linked to function in many experimental systems, its mechanism action remains unclear. Here, we revealed cargo-adapter-mediated activation model organism Aspergillus nidulans Specifically, found that overexpressed cargo adapter HookA (Hook A. nidulans) missing cargo-binding domain (ΔC-HookA) regulator dynactin relocate from plus ends minus...
The functional significance of Kinesin-1 autoinhibition has been unclear. transports multiple cargoes including cytoplasmic dynein to microtubule plus ends. From a genetic screen for Aspergillus mutants defective in dynein-mediated early endosome transport, we identified kinesin-1 mutation kinAK895* at the C-terminal IAK motif involved autoinhibition. kinA∆IAK and kinAK895E exhibited similar defect verifying importance transport. is not critical accumulation ends or secretory vesicle reach...
Cytoplasmic dynein-mediated intracellular transport needs the multi-component dynactin complex for cargo binding and motor activation. However, cellular factors involved in assembly remain unexplored. Here we found Aspergillus nidulans that vezatin homolog VezA is important assembly. affects microtubule plus-end accumulation of dynein before adapter-mediated activation, two processes both need dynactin. The contains multiple components including an Arp1 (actin-related protein 1)...
Cytoplasmic dynein-mediated intracellular transport needs the multi-component dynactin complex for cargo binding and motor activation. However, cellular factors involved in assembly remain unexplored. Here, we found Aspergillus nidulans that vezatin homolog VezA is important assembly. affects microtubule plus-end accumulation of dynein before cargo-adapter-mediated activation, two processes both need dynactin. The contains multiple components, including p150, p50, an Arp1 (actin-related...
Abstract DNA double strand breaks (DSBs), neuroinflammation, and vascular alterations in the brain are all associated with neurodegenerative disorders. However, interconnections between these neuropathological changes how they act synergistically to promote irreversible neurodegeneration remain unclear. Here we show that abrogating BRCA1-associated protein Brap cerebral cortical neurons, as opposed endothelium cells, causes cerebrovascular defects. This non-cell autonomous effect is mediated...