A5085016805- Microtubule and mitosis dynamics
- Cellular transport and secretion
- Protist diversity and phylogeny
- Fungal and yeast genetics research
- Photosynthetic Processes and Mechanisms
- Dermatology and Skin Diseases
- 14-3-3 protein interactions
- Psoriasis: Treatment and Pathogenesis
- Plant Reproductive Biology
- Autoimmune Bullous Skin Diseases
- Skin and Cellular Biology Research
- Ubiquitin and proteasome pathways
- RNA regulation and disease
- Genetic and rare skin diseases.
- DNA and Nucleic Acid Chemistry
- Autoimmune and Inflammatory Disorders Research
- Ferroptosis and cancer prognosis
- Water Quality and Pollution Assessment
- melanin and skin pigmentation
- Histiocytic Disorders and Treatments
- Fungal Infections and Studies
- Plant-Microbe Interactions and Immunity
- Telomeres, Telomerase, and Senescence
- Dermatological and COVID-19 studies
- Histone Deacetylase Inhibitors Research
Guizhou University
2024-2025
Beijing Children’s Hospital
2012-2025
Capital Medical University
2018-2025
Qinghai University
2024-2025
Ministry of Education of the People's Republic of China
2024-2025
Uniformed Services University of the Health Sciences
2015-2024
Beijing Sanbo Brain Hospital
2022-2024
Wenzhou Medical University
2024
First Affiliated Hospital of Wenzhou Medical University
2024
Beijing Shijitan Hospital
2024
Mine water influx is a significant geological hazard during mine development, influenced by various factors such as conditions, hydrology, climate, and mining techniques. This phenomenon characterized non-linearity high complexity, leading to frequent accidents in coal mines. These not only impact production quality but also jeopardize the safety of staff. In order better predict amount surging mines provide an important basis for damage prevention work, based on time series data from...
Nuclear migration plays an important role in the growth and development of many organisms including multinuclear fungus Aspergillus nidulans. We have identified four genes, nudA, nudC, nudF, nudG, which temperature-sensitive mutations affect nuclear distribution. In this report, we describe cloning nudA gene by complementation mutant phenotype using a chromosome VIII-specific cosmid library. A genomic fragment hybridized to mRNA approximately 14 kb. Sequencing analysis revealed ATP-binding...
During a study of the genetics nuclear migration in filamentous fungus Aspergillus nidulans, we cloned gene, nudF, which is required for during vegetative growth as well development. The NUDF protein level controlled by another NUDC, and extra copies nudF gene can suppress nudC3 mutation. encodes with 42% sequence identity to human LIS-1 (Miller-Dieker lissencephaly-1) proper neuronal brain This strong similarity suggests that product may have function similar supports previous findings...
Cytoplasmic dynein transports membranous cargoes along microtubules, but the mechanism of dynein–cargo interaction is unclear. From a genetic screen, we identified homologue human Hook proteins, HookA, as factor required for dynein-mediated early endosome movement in filamentous fungus Aspergillus nidulans. HookA contains putative N-terminal microtubule-binding domain followed by coiled-coil domains and C-terminal cargo-binding domain, an organization reminiscent cytoplasmic linker proteins....
Deficiency of the LIS1 protein causes lissencephaly, a brain developmental disorder. Although binds microtubule motor cytoplasmic dynein and has been linked to function in many experimental systems, its mechanism action remains unclear. Here, we revealed cargo-adapter-mediated activation model organism Aspergillus nidulans Specifically, found that overexpressed cargo adapter HookA (Hook A. nidulans) missing cargo-binding domain (ΔC-HookA) regulator dynactin relocate from plus ends minus...
The mechanism(s) by which microtubule plus-end tracking proteins are targeted is unknown. In the filamentous fungus Aspergillus nidulans, both cytoplasmic dynein and NUDF, homolog of LIS1 protein, localize to plus ends as comet-like structures. Herein, we show that NUDM, p150 subunit dynactin, also forms dynamic structures at ends. By examining tagged with green fluorescent protein in different loss-of-function mutants, demonstrate dynactin require each other for accumulation, presence...
The mechanism(s) by which proteins traverse and exit the Golgi are incompletely understood. Using Aspergillus nidulans hyphae, we show that late cisternae undergo changes in composition to gradually lose identity while acquiring post-Golgi RabE RAB11 identity. This behavior of is consistent with cisternal maturation model. Post-Golgi carriers travel to, accumulate at, apex, indicating fusion rate limiting for exocytosis. These carriers, loaded kinesin, dynein, MyoE MYO5 , move on a...
The objective of this study was to identify existing clinical compounds that either possess a fungicidal activity alone or can act synergistically with fungistatic antifungals. We screened compound library for drugs exhibited anti-Aspergillus activity. Among selected compounds, the cationic peptide antibiotic polymyxin B chosen further characterization because it be used parenterally and topically. effect its synergistic interactions azole antifungals were tested against variety fungal...
Cytoplasmic dynein transports various cellular cargoes including early endosomes, but how is linked to endosomes unclear. We find that the Aspergillus nidulans orthologue of p25 subunit dynactin critical for dynein-mediated endosome movement not nuclear distribution. In absence NUDF/LIS1, deletion abolished localization dynein–dynactin hyphal tip where abnormally accumulate did prevent microtubule plus ends. Within complex, locates at pointed end Arp1 filament with Arp11 and p62, our data...
Migration of nuclei throughout the mycelium is essential for growth and differentiation filamentous fungi. In Aspergillus nidulans, nudA gene, which involved in nuclear migration, encodes a cytoplasmic dynein heavy chain. this paper we use antibodies to characterize chain (ACDHC) show that ACDHC concentrated at growing tip fungal mycelium. We demonstrate four temperature-sensitive mutations gene result striking decrease protein. Cytoplasmic has been implicated division animal cells. Because...
A founder transgenic mouse harbored two different integration patterns of a transgene at the same locus, each which gave rise to similar autosomal recessive mutation. Mice mutant phenotype were small stature but had normal levels growth hormone. The disrupted locus was cloned, and genetic molecular analysis showed that insertional mutants allelic spontaneous mutant, pygmy. mice should be useful model for hormone-resistant human dwarf syndromes could lead greater understanding pathways...
Cytoplasmic dynein in filamentous fungi accumulates at microtubule plus-ends near the hyphal tip, which is important for minus-end-directed transport of early endosomes. It was hypothesized that switched on plus-end by cargo association. Here, we show Aspergillus nidulans kinesin-1-dependent localization not a prerequisite ATPase activation. First, Walker A and B mutations heavy chain AAA1 domain implicated blocking different steps cycle cause effects to microtubules, arguing against...
The minus end-directed microtubule motor cytoplasmic dynein transports various cellular cargoes, including early endosomes, but how binds to its cargo remains unclear. Recently fungal Hook homologues were found link endosomes for their transport. Here we identified FhipA in Aspergillus nidulans as a key player HookA (A. Hook) function via genome-wide screen mutants defective early-endosome distribution. human homologue of FhipA, FHIP, is protein the previously discovered FTS/Hook/FHIP (FHF)...
Hyphal tip cells of the fungus Aspergillus nidulans are useful for studying long-range intracellular traffic. Post-Golgi secretory vesicles (SVs) containing RAB11 orthologue RabE engage myosin-5 as well plus end- and minus end-directed microtubule motors, providing an experimental system with which to investigate interplay between actin motors acting on same cargo. By exploiting fact that depolymerization F-actin unleashes SVs focused at apex by microtubule-dependent we establish transport...
A Gram-stain-positive, aerobic and non-motile actinobacterium, designated strain MR15.9 T , was isolated from sediment collected a mangrove ecosystem in Semarang city, Indonesia. Strain grew at 4–37 °C (optimum 30 °C), pH 6.0–11.0 7.0–8.0), with 0–10% (w/v) NaCl 0–3%). The genome of 3.67 Mbp 71.7 mol% G+C content. Phylogenetic analysis based on 16S rRNA gene sequence directed that formed well-supported clade Tersicoccus solisilvae CGMCC 1.15480 phoenicis 1P05MA shared the highest similarity...