A5034429217- Fungal and yeast genetics research
- Microtubule and mitosis dynamics
- Nuclear Structure and Function
- Protist diversity and phylogeny
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- 14-3-3 protein interactions
- Biofuel production and bioconversion
- Ubiquitin and proteasome pathways
- Cellular transport and secretion
- Microbial Metabolic Engineering and Bioproduction
- Glycosylation and Glycoproteins Research
- Enzyme Production and Characterization
- Photosynthetic Processes and Mechanisms
- Plant Pathogens and Fungal Diseases
- Antifungal resistance and susceptibility
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Enzyme-mediated dye degradation
- Enzyme Structure and Function
- RNA regulation and disease
The Ohio State University
2011-2022
Karlsruhe Institute of Technology
2010
Geisinger Medical Center
1994-2002
Eli Lilly (United States)
1999-2001
Pennsylvania State University
1997-2000
Penn State Milton S. Hershey Medical Center
2000
Baylor College of Medicine
1991-1999
University of Kentucky
1994-1998
Rutgers, The State University of New Jersey
1987-1995
Duke University
1993
Neurospora crassa is a central organism in the history of twentieth-century genetics, biochemistry and molecular biology. Here, we report high-quality draft sequence N. genome. The approximately 40-megabase genome encodes about 10,000 protein-coding genes--more than twice as many fission yeast Schizosaccharomyces pombe only 25% fewer fruitfly Drosophila melanogaster. Analysis gene set yields insights into unexpected aspects biology including identification genes potentially associated with...
Aspergillus nidulans is an important experimental organism, and it a model organism for the genus that includes serious pathogens as well commercially organisms. Gene targeting by homologous recombination during transformation possible in A. nidulans, but frequency of correct gene variable often low. We have identified homolog (nkuA) human KU70 essential nonhomologous end joining DNA double-strand break repair. Deletion nkuA (nkuA delta) greatly reduces integration transforming fragments,...
Hyphal tip growth in fungi is important because of the economic and medical importance fungi, it may be a useful model for polarized other organisms. We have investigated central questions roles cytoskeletal elements precise sites exocytosis endocytosis at growing hyphal by using fungus Aspergillus nidulans. Time-lapse imaging fluorescent fusion proteins reveals remarkably dynamic, but highly structured, apparatus. Live SYNA, synaptobrevin homologue, SECC, an exocyst component, that vesicles...
During a study of the genetics nuclear migration in filamentous fungus Aspergillus nidulans, we cloned gene, nudF, which is required for during vegetative growth as well development. The NUDF protein level controlled by another NUDC, and extra copies nudF gene can suppress nudC3 mutation. encodes with 42% sequence identity to human LIS-1 (Miller-Dieker lissencephaly-1) proper neuronal brain This strong similarity suggests that product may have function similar supports previous findings...
The temperature-sensitive cell cycle mutation nimA5 causes nuclei of Aspergillus nidulans to be blocked in late G2 at restrictive temperature. Under these conditions the spindle pole body divides but does not separate and mitotic index drops zero. If is for more than one doubling time then shifted from permissive temperature, immediately enter mitosis, forms, chromosomes condense (Oakley, B. R., N. R. Morris, 1983, J. Cell Biol., 96:1155-8). We have cloned wild-type nimA gene by DNA-mediated...
ABSTRACT A method to rapidly generate gene replacement constructs by fusion PCR is described for Aspergillus nidulans . The utility of the approach demonstrated green fluorescent protein (GFP) tagging A. ndc80 visualize centromeres through cell cycle. methodology makes possible large-scale GFP tagging, promoter swapping, and deletion analysis
The filamentous fungi are an ecologically important group of organisms which also have industrial applications but devastating effects as pathogens and agents food spoilage. Protein kinases been implicated in the regulation virtually all biological processes how they regulate fungal specific is not understood. fungus Aspergillus nidulans has long utilized a powerful molecular genetic system recent technical advances made systematic approaches to study large gene sets possible. To enhance A....
A temperature-sensitive mutation in the nudC gene (nudC3) of Aspergillus nidulans specifically prevents microtubule-based movement nuclei this organism at restrictive temperature. The does not affect short term growth, nuclear division, or other subcellular organelles. Immunofluorescence analysis cells blocked temperature, using antitubulin antibodies, shows that inability to move under these conditions is related an a particular class microtubule form. mutant also shown be independent both...
To define the extent of modification nuclear pore complex (NPC) during Aspergillus nidulans closed mitosis, a systematic analysis transport genes has been completed. Thirty have deleted defining 12 nonessential and 18 essential genes. Several deletions caused conditional phenotypes self-sterility, whereas deletion some defects in structure. Live cell imaging endogenously tagged NPC proteins (Nups) revealed that mitosis 14 predicted peripheral Nups, including all FG repeat disperse throughout...
NIMA promotes entry into mitosis in late G2 by some mechanism that is after activation of the Aspergillus nidulans cyclin-dependent kinase, NIMXCDC2/NIMECyclin B. Here we present two independent lines evidence which indicate this involves control B localization. First, found NIMECyclin localized to nucleus and nucleus-associated organelle, spindle pole body, a NIMA-dependent manner. Analysis cells from asynchronous cultures, synchronous cultures arrested S or showed was predominantly nuclear...
In Aspergillus nidulans nuclear pore complexes (NPCs) undergo partial mitotic disassembly such that 12 NPC proteins (Nups) form a core structure anchored across the envelope (NE). To investigate how is maintained, we affinity purified major An-Nup84-120 complex and identified two new fungal Nups, An-Nup37 An-ELYS, previously thought to be vertebrate specific. During mitosis locates NE spindle pole bodies but, unlike cells, does not concentrate at kinetochores. We find mutants lacking...