A5087138064- Fungal and yeast genetics research
- Bacterial Genetics and Biotechnology
- Mycotoxins in Agriculture and Food
- RNA and protein synthesis mechanisms
- Plant Pathogens and Fungal Diseases
- Genomics and Phylogenetic Studies
- Microbial Natural Products and Biosynthesis
- Biochemical and Molecular Research
- Polyamine Metabolism and Applications
- Enzyme Structure and Function
- Microbial Metabolic Engineering and Bioproduction
- Fungal Biology and Applications
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Microbial Community Ecology and Physiology
- Protist diversity and phylogeny
- Plant Disease Resistance and Genetics
- Porphyrin Metabolism and Disorders
- Fermentation and Sensory Analysis
- DNA Repair Mechanisms
- Gout, Hyperuricemia, Uric Acid
- Molecular Biology Techniques and Applications
- Genomics and Chromatin Dynamics
- Plant-Microbe Interactions and Immunity
- Trace Elements in Health
Centre National de la Recherche Scientifique
2010-2024
Imperial College London
2015-2024
Institut de Biologie Intégrative de la Cellule
2015-2024
CEA Paris-Saclay
2017-2024
Université Paris-Saclay
2001-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2015-2024
Child Trends
2016-2022
University of Nottingham
2020
Université Paris-Sud
2008-2019
University of Bath
2018
Fungal secondary metabolites are important bioactive compounds but the conditions leading to expression of most putative metabolism (SM) genes predicted by fungal genomics unknown. Here we describe a novel mechanism involved in SM-gene regulation based on finding that, Aspergillus nidulans, mutants lacking components heterochromatin formation show de-repression biosynthesis sterigmatocystin (ST), penicillin and terrequinone A. During active growth phase, silent ST gene cluster is marked...
Summary The molybdenum cofactor (MoCo)‐containing enzymes aldehyde oxidase (AO; EC 1.2.3.1) and xanthine dehydrogenase (XDH; 1.2.1.37) require for activity a sulfuration step that inserts terminal sulfur ligand into the MoCo. tomato flacca mutation was originally isolated as wilty phenotype due to lack of abscisic acid (ABA) is related simultaneous loss AO XDH activities. An expressed sequence tag candidate from selected on basis homology sulfurases animals, fungi recently Arabidopsis genes...
The niaD and niiA genes of Aspergillus nidulans, which code, respectively, for nitrate nitrite reductases, are divergently transcribed, their ATGs separated by 1,200 bp. under the control positively acting NirA transcription factor, mediates induction. DNA binding domain was expressed as a fusion protein with glutathione S-transferase Schistosoma japonicum. Gel shift footprint experiments have shown that in intergenic region there four sites factor. These can be represented nonpalindromic...
The nucleotide sequence of nirA, mediating nitrate induction in Aspergillus nidulans, has been determined. Alignment the cDNA and genomic DNA indicates that gene contains four introns encodes a protein 892 amino acids. deduced NIRA displays all characteristics transcriptional activator. A putative double-stranded DNA-binding domain amino-terminal part comprises six cysteine residues, characteristic for GAL4 family zinc finger proteins. An highly acidic region two proline-rich regions are...
In Aspergillus nidulans, integration of transforming sequences can proceed through recombination with homologous or at heterologous sites in the genome. a strain large deletion gene coding for acetamidase (amdS), plasmid carrying this integrates into and inactivates uapA, putative structural uric acid-xanthine permease, frequency 0.3%. The event occurs 3' to open reading frame amdS. A 10-nucleotide sequence which region is also found within uapA. We have taken advantage clone permease...
Aspergillus mutants resistant to various purine analogues (purine, 8-azaguanine, 2-thioxanthine, and 2-thiouric acid) are defective in at least one step of uptake or breakdown. The properties these show that there two systems for purines, which mediates the hypoxanthine, guanine, adenine, other, xanthine uric acid. Allantoinase-less strains sensitive toxic effects allantoin accumulation. They severely inhibited when grown presence naturally occurring purines. Mutant derived from these, may...
The nucleotide sequence of the uapA gene, coding for uric acid-xanthine permease Aspergillus nidulans, has been determined. predicted gene product comprises 595 amino acids (M(r) 63,365); it is a highly hydrophobic protein with 12-14 putative transmembrane segments and shows no striking similarity to any other membrane either prokaryotes or eukaryotes, except short acid conserved in number different permeases. presence an acidic, amphipathic region overlapping last segment UAPA could also be...
The intron of the mitochondrial apocytochrome b gene, cobA, Aspergillus nidulans has been subjected to sequence analysis. It contains an open reading frame 957 base pairs contiguous with preceding exon. Regions translated frames cobA and third cob gene in yeast show high amino acid homology. Comparison this other introns indicates that codes for a maturase protein splices out encoding it possibly introns. Two very similar decamer peptides are found sequences intron, four introns, 1 (RF-1)...
This paper describes the isolation and properties of a class mutants ( apl A c ), constitutive for enzyme xanthine dehydrogenase. These are superinducible semidominant. Four independently isolated strains carry mutations which allelic. Strains carrying mutation are, as wild type, repressible by ammonia, at variance with type nitrate. The suppress hx locus that result in loss dehydrogenase, ua Y non‐inducibility both dehydrogenase urate oxidase, but only Immunological evidence shows results...
NCS1 proteins are H(+)/Na(+) symporters specific for the uptake of purines, pyrimidines and related metabolites. In this article, we study origin, diversification substrate specificity fungal transporters. We show that two sub-families, Fur Fcy, plant homologues originate through independent horizontal transfers from prokaryotes expansion by gene duplication led to functional NCS1. characterised all model fungus Aspergillus nidulans discovered novel functions specificities. Homology...
ABSTRACT In the fungus Aspergillus nidulans , inactivation of flbA to -E fluG fluF and tmpA genes results in similar phenotypes, characterized by a delay conidiophore asexual spore production. flbB -D encode transcription factors needed for proper expression brlA gene, which is essential development. However, recent evidence indicates that FlbB FlbE also have nontranscriptional functions. Here we show fluF1 an allele flbD R47P substitution. Amino acids C46 R47 are highly conserved FlbD many...