A5026285748- Fungal and yeast genetics research
- Microbial Natural Products and Biosynthesis
- Inorganic and Organometallic Chemistry
- Metal complexes synthesis and properties
- Microbial Metabolic Engineering and Bioproduction
- Chemical Reaction Mechanisms
- Mycotoxins in Agriculture and Food
- Bacterial Genetics and Biotechnology
- Free Radicals and Antioxidants
- Enzyme Production and Characterization
- Organometallic Compounds Synthesis and Characterization
- Enzyme Structure and Function
- Fungal Biology and Applications
- Radioactive element chemistry and processing
- Polyamine Metabolism and Applications
- Chemical and Physical Properties in Aqueous Solutions
- Urban Transport and Accessibility
- Analytical Chemistry and Chromatography
- Plant Disease Resistance and Genetics
- Biofuel production and bioconversion
- Molecular Sensors and Ion Detection
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Organic Chemistry Cycloaddition Reactions
- Plant nutrient uptake and metabolism
Ollscoil na Gaillimhe – University of Galway
2014-2025
The University of Melbourne
2008-2022
National University of Ireland
1998-2015
RTX (United States)
2012
Howard Hughes Medical Institute
2005
Duke University Hospital
2005
Duke Medical Center
2005
Monash University
2002
La Trobe University
1972-2000
Alltech (Ireland)
1993-1998
A computer program has been elaborated which uses the complexation-induced displacements of NMR chemical shifts to calculate stability constants for general reaction (i) gives generalised constant (ii). The can deal with data from a wide variety reactions including proton mM +nL +jH ⇌ MmHjLn(i), βmjn=[MmHjLn]/[M]m[H]j[L]n(ii) equilibria, metal-ion hydrolysis and metal–ligand interactions. It also situations where both ligand equilibria complex-formation must be considered.
Aspergillus nidulans is an important experimental organism, and it a model organism for the genus that includes serious pathogens as well commercially organisms. Gene targeting by homologous recombination during transformation possible in A. nidulans, but frequency of correct gene variable often low. We have identified homolog (nkuA) human KU70 essential nonhomologous end joining DNA double-strand break repair. Deletion nkuA (nkuA delta) greatly reduces integration transforming fragments,...
Previous analysis of the amdS gene Aspergillus nidulans has identified multiple regulatory circuits mediated by trans-acting genes, cis-acting mutations have been and shown to specifically affect individual circuits. Fine-structure genetic mapping regions showed that these occur in a complex controlling region adjacent structural gene. The was cloned differential hybridization, using cDNA probes derived from high-level-producing strain with large deletion mutation. RNA blotting experiments...
1H NMR spectroscopic data and complementary theoretical predictions suggest that a designed receptor exhibits the anion–π interaction in solution.
Summary Fungi can use a diverse range of nitrogen sources. Some sources sustain rapid growth rate and are used in preference to less readily metabolized The mechanisms involved this control utilization have been studied the model filamentous ascomycete, Aspergillus nidulans. GATA transcription factor AreA is necessary for expression nitrogen‐catabolic permeases enzymes. activity controlled by multiple including regulated areA transcript levels nuclear export. During sufficiency, activation...
Towns and cities worldwide are challenging the dominance of private cars seeking ways to limit their use, compelled by urgency deal with climate crisis. There is a growing determination transition more active sustainable transport such as walking, cycling, public transport, reduce our overall need for car use in urban areas while improving health local environs. Cycling low-impact aerobic exercise mobility option that can assist attempts cut harmful emissions, moderate economically damaging...
The gene nmrA of Aspergillus nidulans has been isolated and found to be a homolog the Neurospora crassa nmr-1, involved in nitrogen metabolite repression. Deletion results partial derepression activities subject repression similar phenotypes observed for certain mutations positively acting areA gene.
ABSTRACT The catabolism of fatty acids is important in the lifestyle many fungi, including plant and animal pathogens. This has been investigated Aspergillus nidulans , which can grow on acetate as sources carbon, resulting production acetyl coenzyme A (CoA). Acetyl-CoA metabolized via glyoxalate bypass, located peroxisomes, enabling gluconeogenesis. Acetate induction enzymes specific for utilization well bypass Zn 2 -Cys 6 binuclear cluster activator FacB. However, acid beta-oxidation...
Peroxisomes are organelles containing a diverse array of enzymes. In fungi they important for carbon source utilization, pathogenesis, development, and secondary metabolism. We have studied Aspergillus nidulans peroxin (pex) mutants isolated by virtue their inability to grow on butyrate or the inactivation specific pex genes. While all able form colonies, those unable import PTS1 proteins partially defective in asexual sexual development. The acetate but affected growth fatty acids,...
There is a single major alcohol dehydrogenase (ADH) and aldehyde (AldDH) in Aspergillus nidulans . Both ADH AldDH are induced by ethanol acetaldehyde both subject to carbon catabolite repression. necessary for the utilization of threonine, indicating that compounds utilized via acetaldehyde. each give activity band on gel electrophoresis. Sodium dodecyl sulphate polyacrylamide electrophoresis cell extracts shows at least two similar polypeptides approximate relative molecular mass (r. m. m.)...
Acetyl coenzyme A (CoA) is a central metabolite in carbon and energy metabolism the biosynthesis of cellular molecules. source cytoplasmic acetyl-CoA essential for production fatty acids sterols protein acetylation, including histone acetylation nucleus. In Saccharomyces cerevisiae Candida albicans produced from acetate by synthetase, while plants animals derived citrate via ATP-citrate lyase. filamentous ascomycete Aspergillus nidulans, tandem divergently transcribed genes (aclA aclB)...
Summary Siderophores play a central role in iron metabolism and virulence of most fungi. Both A spergillus fumigatus nidulans excrete the siderophore triacetylfusarinine C ( TAFC ) for acquisition. In . , green fluorescence protein‐tagging revealed peroxisomal localization biosynthetic enzymes SidI (mevalonyl‐ CoA ligase), SidH hydratase) SidF (anhydromevalonyl‐ transferase), while elimination targeting signal PTS impaired both, ‐targeting biosynthesis. The analysis mutants deficient...
ABSTRACT Both the availability and quality of nutrients affect cellular functions by controlling gene activity. AreA, a member GATA family transcription factors, globally activates expression genes involved in nitrogen source utilization Aspergillus nidulans. The determines level activation capacity AreA through controls at areA mRNA stability interaction with corepressor NmrA. potential sources also affects AreA. We show that complete absence results an enhanced AreA-dependent this response...
Abstract Medical implants and devices are now used successfully in surgical procedures on a daily basis. Alloys of nickel titanium, particular Nitinol special interest the medical device industry, because their shape memory superelastic properties. The corrosion behavior nitinol body is also critical importance known toxicological effects nickel. stability NiTi alloy physiological environment dependant primarily properties mostly TiO 2 oxide layer that present surface. For study, range wires...
The positively acting regulatory gene amdR of Aspergillus nidulans coordinately regulates the expression four unlinked structural genes involved in acetamide (amdS), omega amino acid (gatA and gabA), lactam (lamA) catabolism. By use DNA-mediated transformation A. nidulans, was cloned from a genomic cosmid library. Southern blot analysis DNA various loss-of-function mutants revealed presence detectable rearrangements, including deletion, an insertion, translocation. No rearrangements were...
The genes encoding the acetate-inducible enzyme acetyl-coenzyme A synthetase from Neurospora crassa and Aspergillus nidulans (acu-5 facA, respectively) have been cloned their sequences compared. predicted amino acid sequence of has 670 residues that either 626 or 606 residues, depending upon which two possible initiation codons is used. following second alternative AUG show 86% homology between species; extended N-terminal no homology. protein characterized by appearance S(T)PXX motif where...