A5085120696- Osteoarthritis Treatment and Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Bone Tissue Engineering Materials
- Orthopaedic implants and arthroplasty
- Proteoglycans and glycosaminoglycans research
- biodegradable polymer synthesis and properties
- Tissue Engineering and Regenerative Medicine
- RNA modifications and cancer
- Nanoparticle-Based Drug Delivery
- Graphene and Nanomaterials Applications
- Silk-based biomaterials and applications
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Bone and Dental Protein Studies
- Dental materials and restorations
- Electromagnetic Fields and Biological Effects
- Dental Implant Techniques and Outcomes
- Silicone and Siloxane Chemistry
- Reconstructive Surgery and Microvascular Techniques
- Neurogenesis and neuroplasticity mechanisms
- Wound Healing and Treatments
- Collagen: Extraction and Characterization
- Estrogen and related hormone effects
Isfahan University of Medical Sciences
2015-2024
Nowadays, cartilage tissue engineering (CTE) is considered important due to lack of repair cartilaginous lesions and the absence appropriate methods for treatment. In this study, polycaprolactone (PCL) scaffolds were fabricated by three-dimensional (3D) printing then coated with fibrin (F) acellular solubilized extracellular matrix (ECM). After extracting adipose-derived stem cells (ADSCs), 3D-printed characterized compared hydrogel groups. inducing chondrogenic differentiation in presence...
Scaffold fabrication methods have a vital role in cartilage tissue engineering (CTE). For long time, extracellular matrix (ECM) derivatives been used to improve chondrogenesis and regeneration. In this study, polycaprolactone (PCL) scaffolds were prepared by 3D-printing salt-leaching methods. Then, the coated with fibrin different acellular solubilised ECM concentrations. Adipose-derived stem cells (ADSCs) added fibrin/ECM hydrogel seeded on PCL scaffolds. Results showed that water...
The purpose of this study was to evaluate the microstructural and mechanical properties poly (Lactide-co-Glycolide) (PLGA) PLGA/ Hyaluronic acid (Ha)/ Fibrin (F)/ Bioactive glass (BG) nanocomposite scaffolds for cartilage tissue engineering. Nanocomposite composed Ha/ F/ BG, containing different amounts Ha, F, BG nanoparticles were prepared via solvent casting particulate leaching (SC/PL) techniques. Characterization techniques such as Fourier transform infrared spectroscopy (FT-IR),...
Background: The generation of bioartificial bone tissues may help to overcome the problems related donor site morbidity and size limitations. Materials Methods: In this paper, hydroxyapatite (HA) powder was made out bovine by thermal analysis at 900°C first, then, porous HA (50 weight percentage) produced polyurethane sponge replication method. order improve scaffold mechanical properties, they have been coated with poly hydroxybutyrate. terms phase studies, morphology, specifying agent...
Carbon nanotubes (CNTs) have a large variety of applications in tissue engineering and biomedical devices. The biocompatibility cytotoxicity CNTs been studied widely, however, up until now; there was uncertainty on how nanosized materials behave the human body stem cells. current study describes functionalized carbon adipose-derived cells (ADSCs) for viability proliferation purposes vitro.After chemical modification CNTs, ADSCs were cultured Dulbecco's Modified Eagle's. Medium (DMEM) having...
There are significant limitations in repair of irrecoverable bone defects. Stem-cell therapy is a promising approach for the construction tissue. Mesenchymal stem cells (MSCs) have been introduced as basic tools tissue generation. Through MSCs, adipose-derived (ADSCs) more interesting. Since similarity native osteoblasts and differentiated from ADSCs terms gene expression pattern unknown, this study was designed to compare patterns some genes involved osteogenesis between human...
The aim of this study was to compare the chondrogenic induction potential Piascledine and TGF-β1 on adipose-derived stem cells (ADSCs) in fibrin fibrin-alginate scaffolds.Human subcutaneous adipose tissues were harvested from three patients who scheduled undergo liposuction. Isolated ADSCs proliferated a culture medium. Then, seeded or scaffolds cultured for 14 days medium containing Piascledine, TGF-β1, both. rate cell proliferation survival evaluated by using MTT [3-(4,...
Background: The aim of this study was to compare the cytotoxicity and biocompatibility three different nanofibers scaffolds after seeding stem cells harvested from human deciduous dental pulp. Given importance scaffold its features in tissue engineering, demonstrated construction polyhydroxybutyrate (PHB)/chitosan/nano-bioglass (nBG) nanocomposite using electrospinning method.Materials Methods: This experimental conducted on normal exfoliated incisors obtained 6-year-old 11-year-old healthy...
In the present research we have applied three types of surface modifications on Ti6Al4 V alloy including heat treatment, H 2 O treatment and alkali treatment. Scanning electron microscope thin-film X-ray diffractometry (TF-XRD) were used to analyse structure formed phases modified specimens. vitro bioactivity evaluations carried out cytocompatibility assay was performed using evaluation viability attachment L-929 fibroblast cells. The results revealed more alkali-treated compared other...
Background: P300 is a member of the mammalian histone acetyl transferase (HAT) family, an enzyme that acetylates histones and several non-histone proteins including P53 (the most important tumor suppressor gene) during stress, which plays role in apoptosis cells. Hereby, this study describes potency CTB (Cholera Toxin B subunit) as activator to induce breast cancer cell line (MCF-7) lung fibroblast (MRC-5) non-tumorigenic control sample.Materials Methods: MCF-7 MRC-5 were cultured RPMI-1640...
Background: Pancreatic cancer has poor prognosis by surgical and chemotherapy when it is diagnosed, so other anti-cancerous assistant therapeutic drugs are suggested e.g. epigenetic reversal of tumor-suppressor genes on promoter hypermethylation. 5-Aza-CdR a nucleoside analog DNMTi but long-term cytotoxicity effects. This study compares the anticancer effect Disulfiram potencies PANC-1 cell line up-regulation p21. Materials Methods : was cultured in DMEM high glucose treated with 5 10 μM...
Background: Low back pain is one of the most significant musculoskeletal diseases our time. Intervertebral disk herniation and central degeneration are two major reasons for low pain, which occur because structural impairment disk. The reduction cell count extracellular matrix, especially in nucleus pulposus, causes degeneration. Different scaffolds have been used tissue repairing regeneration intervertebral engineering. Various methods fabrication porosity freeze drying method has...
Nowadays, cartilage tissue engineering is the best candidate for regeneration of defects. This study evaluates effect fibrin/icariin (ICA) nanoparticles (F/I NPs) on chondrogenesis stem cells.F/I NPs were characterized by Dynamic Light Scattering DLS. Poly (lactic-co-glycolic) acid (PLGA)-F/I NP scaffold was fabricated and assessed scanning electron microscope. Human adipose-derived cells (hADSCs) seeded induced chondrogenesis. After 14 days, cell viability gene expression analyzed 3-(4, 5-...