A5061443036- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- Angiogenesis and VEGF in Cancer
- Mesenchymal stem cell research
- Cellular Mechanics and Interactions
- Additive Manufacturing and 3D Printing Technologies
- Graphene and Nanomaterials Applications
- Orthopaedic implants and arthroplasty
- Microfluidic and Bio-sensing Technologies
- Congenital heart defects research
- Periodontal Regeneration and Treatments
- Cell Image Analysis Techniques
- Bone fractures and treatments
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer Cells and Metastasis
- Advanced Fluorescence Microscopy Techniques
- Polymer Surface Interaction Studies
- Cardiac and Coronary Surgery Techniques
- Cleft Lip and Palate Research
- Cardiac Valve Diseases and Treatments
- Zebrafish Biomedical Research Applications
- Osteoarthritis Treatment and Mechanisms
- Axon Guidance and Neuronal Signaling
University of Twente
2016-2025
Harvard–MIT Division of Health Sciences and Technology
2015-2017
Massachusetts Institute of Technology
2015-2017
Innovation Research Center
2015-2017
Brigham and Women's Hospital
2015-2017
Harvard University
2015-2017
Amsterdam UMC Location Vrije Universiteit Amsterdam
2014
Bronkhorst (Netherlands)
2005-2010
Johannes Gutenberg University Mainz
2008
Wageningen University & Research
2004
To engineer tissues with clinically relevant dimensions, one must overcome the challenge of rapidly creating functional blood vessels to supply cells oxygen and nutrients remove waste products. We tested hypothesis that endothelial cells, cocultured osteoprogenitor can organize into a prevascular network in vitro. When cultured spheroid coculture model human mesenchymal stem umbilical vein (HUVECs) form 3-dimensional within 10 days vitro culture. The formation was promoted by seeding 2% or...
Abstract The supply of oxygen within three‐dimensional tissue‐engineered (TE) cartilage polymer constructs is mainly by diffusion. Oxygen consumption cells results in gradients the concentration. aims this study were, firstly, to identify TE and, secondly, predict profiles during vitro culture. A glass microelectrode system was adapted and used penetrate cartilaginous constructs, yielding reproducible measurements with high spatial resolution. Cartilage were cultured for up 41 days vitro....
Physical forces play a major role in the organization of developing tissues. During vascular development, physical originating from fluid phase or cells pulling on their environment can alter cellular signaling and behavior cells. Here, we observe how tissue deformation spatially modulates angiogenic signals angiogenesis. Using soft lithographic templates, assemble three-dimensional, geometric The tissues contract autonomously, change shape stereotypically form patterns structures regions...
Tissue engineering needs innovative solutions to better fit the requirements of a minimally invasive approach, providing at same time instructive cues cells. The use shape memory polyurethane has been investigated by producing 4D scaffolds via additive manufacturing technology. Scaffolds with two different pore network configurations (0/90° and 0/45°) were characterized dynamic-mechanical analysis. thermo-mechanical analysis showed Tg about 32 °C (Tg = T trans), indicating no influence...
Organ‐on‐a‐chip platforms seek to recapitulate the complex microenvironment of human organs using miniaturized microfluidic devices. Besides modeling healthy organs, these devices have been used model diseases, yielding new insights into pathophysiology. Hutchinson‐Gilford progeria syndrome (HGPS) is a premature aging disease showing accelerated vascular aging, leading death patients due cardiovascular diseases. HGPS targets primarily cells, which reside in mechanically active tissues. Here,...
Abstract Endothelial cells (ECs) are continuously exposed in vivo to cyclic strain and shear stress from pulsatile blood flow. When these stimuli applied vitro , ECs adopt an appearance resembling their state, most apparent alignment (perpendicular uniaxial along the flow). Uniaxial flow perpendicular strain, used studies, only represent conditions straight parts of vessels. The present over large fractions vasculature can be better represented by anisotropic biaxial strains at various...
In vitro vascularization is an upcoming strategy to solve the problem of insufficient blood supply after implantation. Although recent publications show promising results, these studies were generally performed with clinically irrelevant endothelial cell model systems. We tested use progenitor cells (EPC) obtained from umbilical cord and human mesenchymal stem (hMSC) bone marrow for their in a prevascularized tissue engineering setting. MSC differentiated toward cells. They formed...
Engineering bone tissue requires the generation of a highly organized vasculature. Cellular behavior is affected by respective niche. Directing cellular and differentiation for creating mineralized regions surrounded vasculature can be achieved controlling pattern osteogenic angiogenic niches. This manuscript reports on engineering vascularized tissues incorporating cell‐laden niches in photocrosslinkable hydrogel construct. Two‐step photolithography process used to control stiffness...
Given the dynamic nature of engineered vascular networks within biofabricated tissue analogues, it is instrumental to have control over constantly evolving biochemical cues synthetic matrices throughout remodeling. Incorporation pro-angiogenic endothelial growth factor (VEGF165) specific aptamers into cell-instructive polymer shown be pivotal for spatiotemporally controlling local bioactivity VEGF that selectively elicit cell responses. To harness this effect and quantitatively unravel its...
Large bone defects naturally regenerate via a highly vascularized tissue which progressively remodels into cartilage and bone. Current approaches in engineering are restricted by delayed vascularization fail to recapitulate this stepwise differentiation toward tissue. Here, we use the morphogen Sonic Hedgehog (Shh) induce vitro organization of an endothelial capillary network artificial We show that endogenous activity regulates angiogenic genes formation vascular lumens. Exogenous Shh...
Material-free tissues are assembled using solely cells. Microstructured hydrogel templates and high content screening allow the formation of centimeter-scale with precise architectures. Similar to developing tissues, these contract autonomously, controllably shift shape, self-scaffold by secreting extracellular matrix, undergo morphogenesis. As a service our authors readers, this journal provides supporting information supplied authors. Such materials peer reviewed may be re-organized for...
Scaffolds for regenerative medicine applications should instruct cells with the appropriate signals, including biophysical stimuli such as stress and strain, to form desired tissue. Apart from that, scaffolds, especially load bearing applications, be capable of providing mechanical stability. Since both scaffold strength stress-strain distributions throughout depend on scaffold's internal architecture, it is important understand how changes in architecture influence these parameters. In this...
Bubble-powered acoustic microsystems span a plethora of applications that range from lab-on-chip diagnostic platforms to targeted interventions as microrobots. Numerous studies strategize this bubble-powered mechanism generate autonomous self-propulsion microrobots in response high frequency sound waves. Herein, we present two micro-propeller designs which contain an axis-symmetric distribution entrapped bubbles vibrate induce fast rotational motion. Our micro-propellers are synthesized...
IntroductionDue to the loading of articular joints and movement joint itself, mechanical stress is constantly exerted on synovial cavity, which is, in turn, reflected as shear towards surrounding tissues, including synovium. Although it known that cells are sensitive various cues, deeper understanding how fibroblasts respond increased required gain insights its role pathophysiology osteoarthritis (OA).ObjectivesA synovium-on-chip model was developed evaluate effect fluid flow-induced...