Background In clinical dermatology, the identification of subsurface vascular and structural features known to be associated with numerous cutaneous pathologies remains challenging without use invasive diagnostic tools. Objective To present an advanced optical coherence tomography angiography (OCTA) method directly visualize capillary‐level within skin in vivo . Methods An OCTA system a 1310 nm wavelength was used image microvascular various conditions. Subjects were enrolled imaging...

Scaffolds for regenerative medicine applications should instruct cells with the appropriate signals, including biophysical stimuli such as stress and strain, to form desired tissue. Apart from that, scaffolds, especially load bearing applications, be capable of providing mechanical stability. Since both scaffold strength stress-strain distributions throughout depend on scaffold's internal architecture, it is important understand how changes in architecture influence these parameters. In this...

Background: In vivo imaging of the complex cascade events known to be pivotal elements in healing cutaneous wounds is a difficult but essential task. Current techniques are highly invasive, or lack level vascular and structural detail required for accurate evaluation, monitoring treatment. We aimed use an advanced optical coherence tomography (OCT)-based angiography (OCTA) technique non-invasive, high resolution wound healing. Methods: used clinical prototype OCTA image, identify track key...

Skin autografting is a common clinical procedure for reconstructive surgery. Despite its widespread use, very few studies have been conducted to non-invasively evaluate and monitor the vascular structural features of skin grafts. This study, therefore, aims demonstrate potential optical coherence tomography (OCT) alongside OCT-based angiography (OCTA) image human graft health integration over time.An in-house-built prototype OCT system was used acquire OCT/OCTA images from patients who...

Background and Objectives There remains a need to objectively monitor burn wound healing within clinical setting, optical coherence tomography (OCT) is proving itself one of the ideal modalities for just such use. The aim this study utilize noninvasive multipurpose capabilities OCT, along with its cellular‐level resolution, demonstrate application attenuation coefficient (OAC), as derived from OCT data, facilitate automatic digital segmentation epidermis scan images work an objective...

When using optical coherence tomography angiography (OCTA), the development of artifacts due to involuntary movements can severely compromise visualization and subsequent quantitation tissue microvasculatures. To correct such an occurrence, we propose a motion compensation method eliminate from human skin OCTA by means step-by-step rigid affine registration, subpixel nonrigid B-spline registration. accommodate this remedial process, is conducted two matching all-depth volume scans. Affine...

Mineralization in bone tissue involves stepwise cell-cell and cell-ECM interaction. Regulation of osteoblast culture microenvironments can tailor proliferation mineralization rate, the quality and/or quantity final calcified tissue. An vitro model to investigate influencing factors is highly required. We developed a facile which an cell line aggregate (through modification well surfaces) were used mimic intramembranous mineralization. The effect environments including duration (up 72 hours...

There are two forms of system implementation optical coherence tomography angiography (OCTA) in ophthalmic imaging, i.e., spectral domain (SD-) and swept source OCTA (SS-OCTA). The purpose this paper is to compare the SD-OCTA SS-OCTA for elucidating structural vascular features associated with central serous retinopathy (CSR), evaluate effects CSR on SD- SS-OCTA's imaging capabilities. Normal subjects patients were imaged by using 3 × mm 6 scan patterns. OCT signal strengths at superficial...

Objectives To measure and compare microvascular responses within the skin of upper arm to local stimuli, such as heating or rubbing, through use optical coherence tomography angiography (OCTA), investigate its impact on blood volume collection. Materials Methods With heat packs stimulation was applied either left right arm. Data from stimulated sites were obtained using OCTA comparing pre‐ post‐stimulation parameters, vessel density, mean diameter, avascular pore size. Additionally,...

The visual assessment of burned skin is inherently subjective, and whilst a number imaging modalities have identified quantifiable parameters to characterize vascular structural changes following burn damage, none become common place in the protocol. Here, we use optical coherence tomography (OCT)-based angiography (OCTA) introduce novel correlations between vessel depth, i.e., depth functional blood vessels beneath tissue surface, edema interstitial fluid buildup injury collagen...

Background and Objectives There is a clinical need for an accurate, non‐invasive imaging tool that can provide the objective assessment of burn wounds. The aims this study are to demonstrate potential optical coherence tomography (OCT) in evaluating wound healing, as well exploring physiological basis human healing. Study Design/Materials Methods This was retrospective study. Seven patients with severe wounds who were admitted Harborview Medical Center imaged using in‐house‐built,...

The cerebral vascular system provides a means to meet the constant metabolic needs of neuronal activities in brain. Within capillary bed, interactions spatial and temporal hemodynamics play deterministic role oxygen diffusion, however, progression which remains unclear. Taking advantages high-spatiotemporal resolution optical coherence tomography velocimetry designed with eigen-decomposition statistical analysis, we investigated intrinsic red blood cell (RBC) velocities their spatiotemporal...

Having advantageous biocompatibility and osteoconductive properties known to enhance the osteogenic differentiation of mesenchymal stem cells (MSCs), hydroxyapatite (HA) is a commonly used material for bone tissue engineering. What remains unclear, however, whether HA holds similar potential stimulating MSCs that more frequently osteogenic-inducing medium (OIM). To end, we PHBV electrospun nanofibrous scaffolds directly compare capacities with OIM over MSCs. Through observation cellular...

Micro-mass culturing or cellular aggregation is an effective method used to form mineralised bone tissue. Poor core cell viability, however, often impeding characteristic of large micro-mass cultures, and equally for tissue-engineered grafts. Because this, efforts are being made enhance graft perfusion, through pre-vascularisation, which involves the co-culture endothelial cells stem cells. This study investigated effects different techniques culture conditions on arrangements in mesenchymal...

Penetrating vessels bridge the mesh of communicating on surface cortex with subsurface microvascular beds that feed underlying neural tissue. Their accurate identification in vivo is important investigations degenerative diseases, e.g., Alzheimer's disease and stroke. Here, we propose an efficient method to automatically map cortical penetrating based eigen decompensation analysis optical coherence tomography (OCT) OCT angiographic signals. We first project ensemble repeated signals into a...

Background: Examination of the skin's vascular and structural features is essential in clinical, medical, research dermatology. However, there a lack comprehensive imaging tools that clearly accurately evaluates features. Current techniques are invasive have inherent preparatory drawbacks. Aim: To use optical coherence tomography angiography (OCTA) for more accurate depiction vessels skin without dye or other to visualize asses role vasculature during process' wound healing. Method: We used...

Our previous research has demonstrated that minor adjustments to in vitro cellular aggregation parameters, i.e. alterations aggregate size, can influence temporal and spatial mineral depositions within maturing bone cell nodules. What remains unclear, however, is how size might affect mineralisation said nodules over long-term vivo culture. In this study, we used an osteoblast line, MLO-A5, a primary culture, mesenchymal stem cells (MSC), compare small (approximately 80 µm) with large 220...

Abstract Introduction Accurate assessment of burn depth and healing time remains elusive. As a result, some patients may wait weeks, through painful wound care only to have surgery. Others early surgery on wounds that healed with good outcome. Expert opinion the most common method evaluation. We sought evaluate if Optical Coherence Tomography (OCT) Microangiography (OMAG) can predict healing. Methods prospectively enrolled cohort 17 burned subjects whose attending physician initially...