A5001337359- X-ray Diffraction in Crystallography
- Synthetic Organic Chemistry Methods
- Asymmetric Synthesis and Catalysis
- Crystallization and Solubility Studies
- Sulfur-Based Synthesis Techniques
- Chemical Synthesis and Analysis
- Synthesis and properties of polymers
- Crystallography and molecular interactions
- Organic and Inorganic Chemical Reactions
- Catalytic C–H Functionalization Methods
- Carbon dioxide utilization in catalysis
- Click Chemistry and Applications
- Oxidative Organic Chemistry Reactions
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nanoparticle-Based Drug Delivery
- Molecular Junctions and Nanostructures
- Catalytic Cross-Coupling Reactions
- Organometallic Complex Synthesis and Catalysis
- N-Heterocyclic Carbenes in Organic and Inorganic Chemistry
- Nanoplatforms for cancer theranostics
- RNA Interference and Gene Delivery
- Synthesis of heterocyclic compounds
- Cancer-related gene regulation
- Synthesis and Biological Evaluation
- Photodynamic Therapy Research Studies
University of Victoria
2016-2025
University of Bath
2023
Institut für Angewandte Trainingswissenschaft
2023
University of Calgary
2002-2015
Augusta University
2015
Imperial College London
2015
Kitasato University
2015
Harvard University
2007
Triclosan (TCS) and triclocarban (TCC) are widely used broad spectrum bactericides that common pollutants of waterways soils. Methyl triclosan (mTCS) is the predominant bacterial TCS metabolite. Previous studies have shown disrupts thyroid hormone (TH) action; however, effects mTCS or TCC not known. The present study uses cultured frog tadpole tail fin biopsy (C-fin) assay TH-responsive rat pituitary GH3 cell line to assess these three chemicals (1–1000 nM) on TH signaling cellular stress...
Addition of molecular cross-links to polymers increases mechanical strength and improves corrosion resistance. However, it remains challenging install in low-functionality macromolecules a well-controlled manner. Typically, high-energy processes are required generate highly reactive radicals situ, allowing only limited control over the degree type cross-link. We rationally designed bis-diazirine molecule whose decomposition into carbenes under mild controllable conditions enables...
We report here a peptide-driven approach to create first inhibitors of the chromobox homolog 7 (CBX7), methyllysine reader protein. CBX7 uses its chromodomain bind histone 3, lysine 27 trimethylated (H3K27me3), and this recognition event is implicated in silencing multiple tumor suppressors. Small trimethyllysine containing peptides were used as basic scaffold from which potent ligands for disruption CBX7-H3K27me3 complex developed. Potency was determined by fluorescence polarization and/or...
Diazirine reagents allow for the ready generation of carbenes upon photochemical, thermal, or electrical stimulation. Because formed in this way can undergo rapid insertion into any nearby C-H, O-H N-H bond, molecules that encode diazirine functions have emerged as privileged tools applications ranging from biological target identification and proteomics through to polymer crosslinking adhesion. Here we use a combination experimental computational methods complete first comprehensive survey...
Motivated by a desire to develop flexible covalent adhesives that afford some of the same malleability in adhesive layer as traditional polymer-based adhesives, we designed and synthesized two flexible, highly fluorinated bis-diazirines. Both molecules are shown function effective crosslinkers for polymer materials, act strong when painted between objects low surface energy, prior thermal activation. Data obtained from lap-shear experiments suggests greater molecular flexibility is...
Ultrahigh-molecular-weight polyethylene (UHMWPE) fibers are valued for their high strength-to-weight ratio and employed in a wide variety of commercial applications. However, because its low surface energy, UHMWPE can fail certain applications such as fiber-reinforced composites. This is due to poor interfacial adhesion with polar matrices, making it challenging incorporate into composites or retain dyes colorants. Herein, we describe the use polyamine primers coating weaves, covalently...
Colloidal quantum dots (QDs) with a wide color gamut and high luminescent efficiency are promising for next-generation electronic photonic devices. However, precise scalable patterning of QDs without degrading their properties integration into commercially relevant devices, such as digitally addressable QD light-emitting diode (QLED) displays, remain challenging. Here, we develop electronically optimized diazirine-based cross-linkers nondestructive, direct photopatterning and, ultimately,...
Here we present evidence that (+)-avrainvillamide, a naturally occurring alkaloid with antiproliferative effects, binds to the nuclear chaperone nucleophosmin, proposed oncogenic protein is overexpressed in many different human tumors. Among other nucleophosmin known regulate tumor suppressor p53. A synthetic biotin−avrainvillamide conjugate, nearly equipotent natural product inhibiting growth of cultured T-47D cells, was used for affinity-isolation identified as by MS sequencing and...
Covalently crosslinked polymeric materials, known as thermosets, possess enhanced mechanical strength and thermal stability relative to the corresponding uncrosslinked thermoplastics. However, presence of covalent inter-chain crosslinks that makes thermosets so attractive is precisely what them difficult reprocess recycle. Here, we demonstrate introduction chemically cleavable groups into a bis-diazirine crosslinker. Application this crosslinker reagent commercial low-functionality...
Abstract Nucleobase analogue (NBA) drugs, such as 5-fluorouracil (5-FU), are effective chemotherapeutics, but their clinical use is limited by severe side effects. Compelling evidence suggests that the of S-methyl-5'-thioadenosine (MTA) can selectively reduce NBA toxicity on normal tissues while maintaining efficacy NBAs methylthioadenosine phosphorylase (MTAP)-deficient cancers. However, we found MTA induced hypothermia at its dose, limiting translational potential. We hypothesized an...
The polycomb paralogs CBX2, CBX4, CBX6, CBX7, and CBX8 are epigenetic readers that rely on "aromatic cage" motifs to engage their partners' methyllysine side chains. Each CBX carries out distinct functions, yet each includes a highly similar methyllysine-reading chromodomain as key element. CBX7 is the only has been targeted by chemical inhibition. We report small set of peptidomimetic agents in which simple modification switches ligands from one with promiscuity across all provides...
The salicinoids are anti-herbivore phenolic glycosides unique to the Salicaceae (Populus and Salix). They consist of a salicyl alcohol glucoside core, which is usually further acylated with benzoic, cinnamic or acids. While salicinoid structures well known, their biosynthesis remains enigmatic. Recently, two enzymes from poplar, benzoyl transferase benzyl transferase, were shown catalyze production benzoate, predicted potential intermediate in biosynthesis. Here, we used transcriptomics...
We apply gas–liquid microfluidic reactors containing flow-variable, high-shear "hot spots" to produce curcumin-loaded polymer nanoparticles (CUR-PNPs) comprised of poly(caprolactone)-block-poly(ethylene oxide) (PCL-b-PEO) block copolymers at various flow rates and CUR loading ratios. CUR-PNPs prepared using the conventional nanoprecipitation method (bulk method) showed decreased encapsulation efficiency increased drug precipitation as ratio increased. However, by manufacturing both either...
Graphene is a carbon material with extraordinary properties that has been drawing significant amount of attention in the recent decade. High-quality graphene can be produced by different methods, such as epitaxial growth, chemical vapor deposition, and micromechanical exfoliation. The reduced oxide route is, however, only current approach leads to large-scale production materials at reasonable cost. Unfortunately, reduction normally yields high defect density. Here, we introduce new for...
Polyurethane coatings have strong material properties due to the hydrogen bonding inherent urethane groups. However, installing this moiety usually requires curing through difficult-to-handle isocyanates. In work, we show development of a polyurethane-based crosslinker that can be used formulate one-component polyurethane coating with similar those isocyanate-based coatings. To achieve this, diazirine functionalities generate carbenes upon heating, which react alcohol in polyol crosslinked...
We report that the dimeric alkaloid stephacidin B (1) and monomeric avrainvillamide (2) function equivalently within experimental error to inhibit growth of four different cultured human cancer cell lines. also show proportion monomer greatly outweighs dimer in medium incubation, half-life for transformation 1 2 is short relative division. Finally, using a monomer-based affinity reagent, we provide evidence interacts with intracellular thiol-containing proteins, likely by covalent modification.
Polydicyclopentadiene (PDCPD) is a tough, heavily crosslinked thermoset polymer that has high heat, chemical, and impact resistance coupled with low density. Current limitations to the broader industrial application of PDCPD include its surface energy lack chemical tunability. Here, we report first example derived from carboxyl-functionalized dicyclopentadiene monomer subsequent thermal crosslinking. The resulting material highest glass-transition temperature reported for...
We demonstrate control of multiscale structure and drug delivery function for paclitaxel (PAX)-loaded polycaprolactone-block-poly(ethylene oxide) (PCL-b-PEO) polymeric nanoparticles (PNPs) via synthesis flow-directed shear processing in a two-phase gas-liquid microfluidic reactor. This strategy takes page from the engineering commodity plastics, where rather than polymer chemistry provides an experimental handle on properties function. PNPs formed copolymers with three different PCL block...
Abstract The SARS-CoV-2 pandemic has highlighted the weaknesses of relying on single-use mask and respirator personal protective equipment (PPE) global supply chain that supports this market. There have been no major innovations in filter technology for PPE past two decades. Non-woven textiles used filtering are products healthcare environment; use protection is focused preventing infection from airborne or aerosolized pathogens such as Influenza A virus SARS-CoV-2. Recently, C–H bond...
Electronically optimized bis-diazirine crosslinkers allow aliphatic polymers to be crosslinked with up 10-fold improved efficacy, relative earlier designs. Activation is achieved using modest temperatures or through UV visible light.