A5037136421- Radiopharmaceutical Chemistry and Applications
- Monoclonal and Polyclonal Antibodies Research
- Cancer Immunotherapy and Biomarkers
- Listeria monocytogenes in Food Safety
- Immunotherapy and Immune Responses
- Escherichia coli research studies
- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Essential Oils and Antimicrobial Activity
- Antifungal resistance and susceptibility
- Microbial Inactivation Methods
- Parkinson's Disease Mechanisms and Treatments
- CAR-T cell therapy research
- Cancer therapeutics and mechanisms
- Antibiotic Resistance in Bacteria
- Synthesis and Biological Evaluation
- Fungal Infections and Studies
- Acute Myeloid Leukemia Research
- Glycosylation and Glycoproteins Research
- Neurological disorders and treatments
- Neuroblastoma Research and Treatments
- Virus-based gene therapy research
- Cancer, Hypoxia, and Metabolism
- Mycotoxins in Agriculture and Food
- Food Safety and Hygiene
University of Saskatchewan
2016-2024
University of British Columbia
2010-2021
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco
2013
University of Victoria
2012-2013
Secretaría de Salud de Jalisco
2013
University of Guelph
2001-2008
Hoag Memorial Hospital Presbyterian
2007
Health Canada
2002
University of Maryland, Baltimore
1994
The human pathogen Listeria monocytogenes is a large concern in the food industry where its continuous detection products has caused string of recalls North America and Europe. Most recognized for ability to grow foods during refrigerated storage, L. can also tolerate several other food-related stresses with some strains possessing higher levels tolerances than others. objective this study was use combination phenotypic analyses whole genome sequencing elucidate potential relationships...
Daratumumab is an anti-CD38 directed monoclonal antibody approved for the treatment of multiple myeloma (MM) and functions primarily via Fc-mediated effector mechanisms such as complement-dependent cytotoxicity (CDC), antibody-dependent cell (ADCC), cellular phagocytosis, T-cell activation. However, not all patients respond to daratumumab therapy management MM remains challenging. Radioimmunotherapy with alpha particle-emitting radionuclides represents a promising approach significantly...
ABSTRACT Listeria monocytogenes strains belonging to serotypes 1/2a and 4b are frequently linked listeriosis. While inlA mutations leading premature stop codons (PMSCs) attenuated virulence common in 1/2a, they rare serotype 4b. We observed PMSCs 35% of L. isolates ( n = 54) recovered from the British Columbia food supply, including (30%), 1/2c (100%), 3a a 3-codon deletion (amino acid positions 738 740) seen 57% fish-processing facilities. Caco-2 invasion assays showed that two with were...
Intestinal epithelial cells (IECs) are a critical component of the innate local immune response. In order to reduce risk pathogen infection or xenobiotic intoxication, different host defense mechanisms have been evolved. Evidence has shown that upon ingestion food feed contaminated with toxins (e.g., mycotoxins), IECs respond by regulating mucin secretions, which act as physical barrier inhibiting bacterial attachment and subsequent infection-related processes. However, effect Fusarium...
ABSTRACT Purpose Despite the availability of new drugs, many patients with acute myeloid leukemia (AML) do not achieve remission and outcomes remain poor. Venetoclax is a promising therapy approved for use in combination hypomethylating agent or low‐dose cytarabine treatment newly diagnosed older AML those ineligible intensive chemotherapy. 225 Actinium‐lintuzumab ( Ac‐lintuzumab) clinical stage radioimmunotherapy targeting CD33 that has shown evidence single‐agent activity...
Multiresistant Salmonella enterica subspecies serovar Typhimurium definitive type 104 (S. DT104 or DT104) bacteria are important pathogens in animals and humans. isolates often called pentaresistant strains that spread clonally. The purpose of this study was to determine phenotypic, genotypic, epidemiologic characteristics 175 S. isolated from food-producing Canada. More than 90% the were resistant ampicillin (Amp), chloramphenicol (Chl), florfenicol (Flo), sulfisoxazole (Sul), tetracycline...
Escherichia coli O157:H7 poses a threat to humans through food- and water-borne transmission. To investigate how environmental stresses affect the transcriptome, we designed targeted microarray consisting of stress response virulence genes (n = 125) analyze impact acidified (pH 3.5), cold (7.5 degrees C), fresh tryptic soy broth (TSB) (37 C) on E. gene expression. Nutrient replenishment with TSB resulted in 72 differentially expressed (> or 1.5-fold change; p < 0.05), 65 induced. All queried...
The human pathogen Listeria monocytogenes continues to pose a challenge in the food industry, where it is known contaminate ready-to-eat foods and grow during refrigerated storage. Increased knowledge of cold-stress response this will enhance ability control food-supply-chain. This study utilized strand-specific RNA sequencing whole cell fatty acid (FA) profiling characterize bacterium's cold stress response. FAs were extracted from cold-tolerant strain at five time points between early lag...
ABSTRACT Defensins are small antimicrobial peptides (AMPs) that play an important role in the innate immune system of mammals. Since effect mycotoxin contamination food and feed on secretion intestinal AMPs is poorly understood, aim this study was to elucidate individual combined effects four common Fusarium toxins, deoxynivalenol (DON), nivalenol (NIV), zearalenone (ZEA), fumonisin B1 (FB1), mRNA expression, protein secretion, corresponding porcine β-defensins 1 2 (pBD-1 pBD-2) using a...
Osteosarcoma (OS) represents 3.4% of all childhood cancers with overall survival 70% not improving in 30 years. The consistent surface overexpression insulin-like growth factor-2 receptor (IGF2R) has been reported commercial and patient-derived xenograft (PDX) OS cell lines. We aimed to assess efficacy safety treating PDX tumors mice radiolabeled antibody IGF2R investigate expression on canine tumors. human lines 143B SaOS2 OS-17, OS-33 OS-31 was evaluated by FACS. biodistribution...
Metastatic melanoma remains difficult to treat despite recent approvals of several new drugs. Recently we reported encouraging results Phase I clinical trial radiolabeled with 188Re murine monoclonal IgM 6D2 melanin in patients Stage III/IV melanoma. Subsequently generated a novel IgG 8C3 melanin. IgGs are more amenable humanization and cGMP (current Good Manufacturing Practice) manufacturing than IgMs. We performed comparative structural analysis melanin-binding 8C3. The therapeutic...
Melanoma is a cancer with increasing incidence and there need for alternatives to immunotherapy within effective approaches treatment of metastatic melanoma. We performed comparative radioimmunotherapy (RIT) experimental B16-F10 melanoma novel humanized IgG melanin h8C3 labeled beta emitter, 177Lu, an alpha-emitter, 213Bi, as well biodistribution, microSPECT/CT imaging, mouse human dosimetry calculations. imaging showed that antibody targets “free” in the tumor microenvironment had high...
Immunotherapy has changed the oncology landscape during last decade and become standard of care for several cancers. The combinations immunotherapy with other treatment modalities are also being investigated. One challenges to investigate such is identify suitable mouse models pre-clinical experiments. In past, we researchers showed that murine B16-F10 melanoma in C57Bl6 mice refractory immune checkpoint inhibitors. this work studied suitability an alternative syngeneic model, Cloudman S91...
Osteosarcoma (OS) is a prevalent primary bone cancer affecting both humans and canines. This study describes initial insights into the interaction of human monoclonal antibody IF3 to an insulin-like growth factor 2 receptor (IGF2R) radiolabeled with either alpha-emitting Actinium-225 (