A5006500200- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- PARP inhibition in cancer therapy
- Mitochondrial Function and Pathology
- Graphite, nuclear technology, radiation studies
- Carcinogens and Genotoxicity Assessment
- Microtubule and mitosis dynamics
- Nuclear and radioactivity studies
- Fungal and yeast genetics research
- 14-3-3 protein interactions
Université Paris-Saclay
2021-2024
Centre National de la Recherche Scientifique
2021-2024
Institut Curie
2019-2024
Université Paris Sciences et Lettres
2020-2024
La Ligue Contre le Cancer
2021-2023
University of Wrocław
2019-2020
Centre National pour la Recherche Scientifique et Technique (CNRST)
2019
Nuclear Pore complexes (NPCs) act as docking sites to anchor particular DNA lesions facilitating repair by elusive mechanisms. Using replication fork barriers in fission yeast, we report that relocation of arrested forks NPCs occurred after Rad51 loading and its enzymatic activity. The E3 SUMO ligase Pli1 acts at safeguard integrity nascent strands generates poly-SUMOylation which promote but impede the resumption synthesis homologous recombination (HR). Anchorage allows removal SENP...
Nuclear pore complexes (NPCs) have emerged as genome organizers, defining a particular nuclear compartment enriched for SUMO protease and proteasome activities, act docking sites the repair of DNA damage. In fission yeast, anchorage perturbed replication forks to NPCs is an integral part recombination-dependent restart mechanism (RDR) that resumes synthesis at terminally dysfunctional forks. By mapping polymerase usage, we report Ulp1-associated ensure efficient initiation restarted...
Abstract Replication stress, characterized by the slowing or stalling of replication forks, is a hallmark many cancers and key driver genomic instability. Therapeutics targeting ATR kinase, central regulator stress response, have emerged as promising strategies for selectively exploiting vulnerabilities cancer cells. A reliable marker essential to better understand its dynamics optimize development application DNA damage response (DDR) inhibitors. Such markers can provide critical insights...
ABSTRACT The SUMO-targeted ubiquitin ligase (STUbL) family is involved in multiple cellular processes via a wide range of mechanisms to maintain genome stability. One the evolutionarily conserved functions STUbL promote changes nuclear positioning DNA lesions, targeting them periphery. In Schizossacharomyces pombe, Slx8 regulator SUMOylated proteins and promotes replication stress tolerance by counteracting toxicity SUMO conjugates. order study dynamic dialectic between ubiquitinylation...
The regulation of telomere and centromere structure function is essential for maintaining genome integrity. Schizosaccharomyces pombe Rrp1 Rrp2 are orthologues Saccharomyces cerevisiae Uls1, a SWI2/SNF2 DNA translocase SUMO-targeted ubiquitin ligase. Here, we show that or overproduction leads to chromosome instability growth defects, reduction in global histone levels mislocalisation centromere-specific Cnp1. These phenotypes depend on putative activities Rrp2, suggesting may be involved...
The SUMO-targeted Ubiquitin ligase (STUbL) family is involved in multiple cellular processes via a wide range of mechanisms to maintain genome stability. One the evolutionarily conserved functions STUbL promote changes nuclear positioning DNA lesions, targeting them periphery. In Schizossacharomyces pombe, Slx8 regulator SUMOylated proteins and promotes replication stress tolerance by counteracting toxicity SUMO conjugates. order study dynamic dialectic between Ubiquitinylation SUMOylation...
Abstract Nuclear pores complexes (NPCs) are genome organizers, defining a particular nuclear compartment enriched for SUMO protease and proteasome activities, acting as docking sites DNA repair. In fission yeast, the anchorage of perturbed replication forks to NPCs is an integral part recombination-dependent restart mechanism (RDR) that resumes synthesis at terminally dysfunctional forks. By mapping polymerase usage, we report Ulp1-associated ensure efficient initiation restarted synthesis,...
ABSTRACT Homologous recombination (HR) is a DNA repair mechanism that ensures, together with heterochromatin machinery, the proper replication, structure and function of telomeres centromeres essential for maintenance genome integrity. Schizosaccharomyces pombe Rrp1 Rrp2 participate in HR are orthologues Saccharomyces cerevisiae Uls1, SWI2/SNF2 translocase SUMO-Targeted Ubiquitin Ligase. We show or upregulation leads to chromosome instability growth defects. These phenotypes depend on...