A5046897574- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Viral gastroenteritis research and epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Virus-based gene therapy research
- Parasitic Infections and Diagnostics
- Trypanosoma species research and implications
- SARS-CoV-2 and COVID-19 Research
- Advanced biosensing and bioanalysis techniques
- Transgenic Plants and Applications
- Sex work and related issues
- Innovative Microfluidic and Catalytic Techniques Innovation
- COVID-19 epidemiological studies
- Animal Disease Management and Epidemiology
- Listeria monocytogenes in Food Safety
- Advanced Biosensing Techniques and Applications
- Mosquito-borne diseases and control
- Reproductive System and Pregnancy
- Animal health and immunology
RenBio (United States)
2020
Aaron Diamond AIDS Research Center
2013-2019
Rockefeller University
2013-2019
University of Michigan
2011-2013
AlphaVax (United States)
2008
University of Massachusetts Chan Medical School
1993
GSK1265744 (GSK744) is an integrase strand-transfer inhibitor that has been formulated as a long-acting (LA) injectable suitable for monthly to quarterly clinical administration. GSK744 LA was administered at two time points 4 weeks apart beginning 1 week before virus administration, and macaques were challenged weekly 8 weeks. LA, plasma concentrations achievable with injections in humans, protected all animals against repeated low-dose challenges. In second experiment, given administration...
Long-acting GSK1265744 (GSK744 LA) is a strand transfer inhibitor of the HIV/SIV (simian immunodeficiency virus) integrase and was shown to be an effective preexposure prophylaxis (PrEP) agent in low-dose intrarectal SHIV (simian-human rhesus macaque challenge model. We examined pharmacokinetics efficacy GSK744 LA as PrEP against repeat high-dose intravaginal female macaques treated with Depo-Provera (depot medroxyprogesterone acetate), which promotes viral transmission vaginally. When...
Twelve neonatal calves were experimentally infected with oocysts of Cryptosporidium parvum. Six in group A fed hyperimmune colostrum at birth had significantly less diarrhea and shed for time than did 6 B from cows that not hyperimmune. Calves 0-4 days (means = 2.3 days), whereas 4-6 5.0 days). 4-9 6.2 7-11 8.5 These findings indicate passive lacteal immunity conferred partial protection against cryptosporidiosis. Whether such was provided by the immunoglobulins highly elevated (greater...
In the absence of an effective HIV-1 vaccine, passive immunization using broadly neutralizing Abs or Ab-like molecules could provide alternative to daily administration oral antiretroviral agents that has recently shown promise as preexposure prophylaxis. Currently, no single Ab (bNAb) combination bNAbs neutralizes all strains at practically achievable concentrations in vivo. To address this problem, we created bispecific combine inhibitory activity ibalizumab (iMab), a humanized mAb...
Nevirapine, a nonnucleoside inhibitor of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase, was administered for the first time to humans in pilot study designed investigate pharmacokinetics and tolerance drug following single-dose administration 21 HIV-1-infected individuals. The followed parallel design. Different groups three subjects each were given one seven dose levels (2.5 400 mg) sequential order, starting with lowest dose. Each subject received only Nevirapine...
A key strategy to a successful vaccine against malaria is identify and develop new adjuvants that can enhance T-cell responses improve protective immunity. Upon co-administration with rodent in mice, 7DW8-5, recently identified novel analog of α-galactosylceramide (α-GalCer), enhances the level malaria-specific immune more strongly than parent compound. In this study, we sought determine whether 7DW8-5 could provide similar potent adjuvant effect on candidate human relevant non-human primate...
We evaluated the effectiveness of cabotegravir (CAB; GSK1265744 or GSK744) long acting as preexposure prophylaxis (PrEP) against intravenous simian immunodeficiency virus (SIV) challenge in a model that mimics blood transfusions based on per-act probability infection.CAB is an integrase strand transfer inhibitor formulated 200 mg/ml injectable nanoparticle suspension effective PrEP agent rectal and vaginal simian/human transmission macaques.Three groups rhesus macaques (n = 8 per group) were...
MK-8591 (4'-ethynyl-2-fluoro-2'-deoxyadenosine [EFdA]) is a novel reverse transcriptase-translocation inhibitor.We assessed as preexposure prophylaxis in the rhesus macaque model of intrarectal challenge with simian/human immunodeficiency virus (SHIV). In study 1, 8 macaques received 3.9 mg/kg orally on day 0 and once weekly for next 14 weeks. Eight controls were treated vehicle. All challenged SHIV109CP3 6 up to 12 challenges or until infection was confirmed. The dose reduced 1.3 0.43...
Immunostimulatory sequences (ISS) are short DNA containing unmethylated CpG dimers that have multiple effects on the host immune system, including ability to stimulate antigen-specific cytotoxic T lymphocytes (CTLs) and drive Th1-type responses. Listeriolysin O (LLO)-containing pH-sensitive liposomes been shown efficiently deliver macromolecules cytosol of APCs CTLs. We hypothesized encapsulating ISS-oligodeoxyribonucleotides (ODNs) in this delivery system would enhance cell-mediated...
Monoclonal antibodies (mAbs) have wide clinical utility, but global access is limited by high costs and impracticalities associated with repeated passive administration. Here, we describe an optimized electroporation-based DNA gene transfer platform technology that can be utilized for production of functional mAbs in vivo, the potential to reduce administration burdens. We demonstrate multiple simultaneously expressed at protective concentrations a protracted period time using doses...
A CD1d-binding glycolipid, α-Galactosylceramide (αGalCer), activates invariant NK T cells and acts as an adjuvant. We previously identified a fluorinated phenyl ring-modified αGalCer analog, 7DW8-5, displaying nearly 100-fold stronger CD1d binding affinity. In the current study, 7DW8-5 was found to exert more potent adjuvant effect than for vaccine based on radiation-attenuated sporozoites of rodent malaria parasite, Plasmodium yoelii, also referred irradiated P. yoelii (IrPySpz). had superb...
For optimal stimulation of T cells, protein-based vaccines must deliver protein antigens to antigen-presenting cells while simultaneously providing immunostimulatory signals. Listeriolysin O (LLO)-containing liposomes have been utilized efficiently the cytosolic pathway for antigen processing and major histocompatibility complex class I-dependent presentation codelivering CpG-oligodeoxyribonuceotides (ODNs). In this report, we describe synthesis lipid-CpG-ODN conjugates utilizing...
Preexposure prophylaxis using antiretroviral agents has been shown to effectively prevent human immunodeficiency virus type 1 (HIV-1) acquisition in high-risk populations. However, the efficacy of these regimens is highly variable, which thought be largely due varying degrees adherence a daily intervention Passive immunization broadly neutralizing antibodies (bNAbs) against HIV-1, with their relatively long half-life and favorable safety profile, could provide an alternative preexposure...
With increasing frequency, humans are facing outbreaks of emerging infectious diseases (EIDs) with the potential to cause significant morbidity and mortality. In most extreme instances, such can become pandemics, as we now witnessing COVID-19. According World Health Organization, this new disease, caused by novel coronavirus SARS-CoV-2, has already infected more than 10 million people worldwide led 499,913 deaths 29 June, 2020. How high these numbers will eventually go depends on many...
BackgroundThe Venezuelan equine encephalitis (VEE) virus replicon system was used to produce virus-like particles (VRP) packaged with a number of different VEE-derived glycoprotein (GP) coats. The GP coat is believed be responsible for the cellular tropism noted VRP and it possible that VEE coats may have affinities cells. We examined in four their ability infect cells vitro induce both humoral immune responses vivo.Methodology/Principal FindingsThe preparations were characterized determine...
Any infectious disease modelling is only as good the data that's fed into computers. Covid-19 a new virus, so modellers started with little and have subsequently had to make assumptions about transmission rates, incubation periods, time gap between when person develops symptoms becomes infectious, recovery rates levels of immunity. The accuracy official coronavirus predictions depend, it seems, on mathematical models. But how accurate are models themselves?