A5041718874- HIV Research and Treatment
- CAR-T cell therapy research
- Virus-based gene therapy research
- T-cell and B-cell Immunology
- Bacillus and Francisella bacterial research
- Immune Cell Function and Interaction
- Cytomegalovirus and herpesvirus research
- Parvovirus B19 Infection Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Viral-associated cancers and disorders
- Toxin Mechanisms and Immunotoxins
- Monoclonal and Polyclonal Antibodies Research
- GABA and Rice Research
- Alcoholism and Thiamine Deficiency
- Advancements in Semiconductor Devices and Circuit Design
- Plant Gene Expression Analysis
- Garlic and Onion Studies
- Click Chemistry and Applications
- Genomics, phytochemicals, and oxidative stress
- Phytochemicals and Antioxidant Activities
- Helminth infection and control
- Bacteriophages and microbial interactions
- Lipid Membrane Structure and Behavior
- Metabolism and Genetic Disorders
- Parasites and Host Interactions
Fate Therapeutics (United States)
2024-2025
Malaghan Institute of Medical Research
2016-2018
Harvard University Press
2015
University of Auckland
2010-2014
Harvard University
2013
Plant & Food Research
2007-2010
Maurice Wilkins Centre
2010
United States Military Academy
2003-2009
AlphaVax (United States)
2008
Claflin University
2006
The goal of an AIDS vaccine regimen designed to induce cellular immune responses should be reduce the viral set point and preserve memory CD4 lymphocytes. Here we investigated whether vaccine-induced immunity in absence any Env-specific antibodies can control replication following multiple low-dose challenges with highly pathogenic SIVmac239 isolate. Eight Mamu-A*01-positive Indian rhesus macaques were vaccinated simian immunodeficiency virus (SIV) gag, tat, rev, nef using a DNA...
The dendritic cell signals required for the in vivo priming of IL-4–producing T cells are unknown. We used RNA sequencing to characterize DCs from skin LN mice exposed two different Th2 stimuli: helminth parasite Nippostrongylus brasiliensis (Nb) and contact sensitizer dibutyl phthalate (DBP)-FITC. Both Nb DBP-FITC induced extensive transcriptional changes that involved multiple DC subsets. Surprisingly, these were highly distinct models, with only a small number genes being similarly...
Cyclamen is a popular and economically significant pot plant crop in several countries. Molecular breeding technologies provide opportunities to metabolically engineer the well-characterized flavonoid biosynthetic pathway for altered anthocyanin profile hence colour of flower. Previously we reported on genetic transformation system cyclamen. Our aim this study was change pigment profiles flower colours cyclamen through suppression 3', 5'-hydroxylase, an enzyme that plays determining role...
Abstract Staphylococcal superantigen-like protein 10 (SSL10) is a highly conserved member of the SSL family secreted by Staphylococcus aureus that displays structural but not functional similarity to superantigens. SSL10 bound fibrinogen and fibronectin from plasma in addition displayed striking specificity toward γ-1 subclass human Igs. also strongly primate IgG any other species tested, including rabbit, pig, guinea cow, sheep, or mouse. A soluble form 12-kDa β-grasp C-terminal domain...
Replication-defective recombinant adenoviruses (rAd) are used as vectors for vaccines well gene therapy. To determine type-specific antibodies to adenovirus (Ad) serotypes 2, 5, 24, 34, and 35, we developed quantitative neutralization assays using with the secreted alkaline phosphatase (SEAP) reporter gene. Among standardized parameters, concentration of infectious noninfectious adenoviral particles in assay is critical a reliable comparison data from different studies. The usefulness this...
Simian-human immunodeficiency virus (SHIV) challenge studies in rhesus macaques were conducted to evaluate the efficacy of adenovirus-based vaccines context different major histocompatibility complex class I genetic backgrounds and vaccine compositions. Mamu-A*01 allele-negative monkeys immunized with one following constructs: (i) replication-defective recombinant adenovirus type 5 (Ad5) expressing human 1 (HIV-1) Tat (Ad5/HIVTat); (ii) Ad5 vector simian (SIV) Gag (Ad5/SIVGag); (iii)...
As part of on-going efforts to control hookworm infection, the "human vaccine initiative" has recognised blood feeding as a feasible therapeutic target for inducing immunity against infection. To this end, molecular approaches have been used identify candidate targets, such Necator americanus (Na) haemoglobinase aspartic protease-1 (APR-1), with immunogenicity profiled in canine and hamster models. We sought accelerate immune analysis these identified targets by developing an appropriate...
The prophylactic efficacies of several multivalent replication-incompetent adenovirus serotype 5 (Ad5) vaccines were examined in rhesus macaques using an intrarectal high-dose simian immunodeficiency virus SIVmac239 challenge model. Cohorts Mamu-A*01(+)/B*17(-) Indian immunized with one combinations Ad5 vectors expressing Gag, Pol, Nef, and Env gp140; for comparison, a Mamu-A*01(+) cohort was the vector alone. There no sign immunological interference between antigens animals. In general,...
One-electron bioreduction of SN30000, a triazine 1,4-dioxide anticancer drug, forms reactive aryl and carbon-centred radicals.
Studying how pathogens subvert the host to cause disease has contributed understanding of fundamental cell biology. Bacillus anthracis, causative agent anthrax, produces virulence factor lethal toxin disarm immunity and pathology. We conducted a phenotypic small molecule screen identify inhibitors toxin-induced macrophage death used an ordered series secondary assays characterize hits determine their effects on cellular function. identified structurally diverse set molecules that act at...
Epidemiological studies have associated coffee consumption with an inverse risk of developing Parkinson's disease, hepatocellular carcinoma and cirrhosis. The molecular mechanisms by which low concentrations the constituents measured in human plasma can reduce incidence such diseases are not clear. Using vitro plasmid DNA system radiolytically generated reactive oxygen species under constant radical scavenging conditions, we shown that chlorogenic acid, its derivatives certain metabolites...
BackgroundThe Venezuelan equine encephalitis (VEE) virus replicon system was used to produce virus-like particles (VRP) packaged with a number of different VEE-derived glycoprotein (GP) coats. The GP coat is believed be responsible for the cellular tropism noted VRP and it possible that VEE coats may have affinities cells. We examined in four their ability infect cells vitro induce both humoral immune responses vivo.Methodology/Principal FindingsThe preparations were characterized determine...
Adenoviral vectors are used widely as gene therapy and vaccine delivery systems. An adenovirus-shedding assay may be performed in clinical trials to monitor the safety of vector investigate potential relation between symptoms shed virus. This report describes development statistical performance shedding assay. Live adenovirus was recovered from throat swab urine samples spiked with E1-deleted type 5 expressing HIV-1 gag [Ad5HIVgag], presence or absence wild-type (WT Ad5). Samples were...
Currently, the most common cases of Kaposi's sarcoma (KS), a form skin cancer, are found in individuals suffering from AIDS. Sequences putative new Human Herpes Virus-Type 8 (HHV-8 or KSHV), identified by Chang, et.al 1994, have been with classical KS, HIV-1 seropositive homosexual men immunodeficiency, and Multi-centric Castleman's Disease. Previously, we shown presence HHV-8 various cell types KS lesions. In present studies, evaluated both sperm specimens negative positive individuals. To...