A5011051154- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Mitochondrial Function and Pathology
- Molecular Biology Techniques and Applications
- Viral gastroenteritis research and epidemiology
- RNA Research and Splicing
- Fungal and yeast genetics research
- RNA modifications and cancer
- Animal Genetics and Reproduction
- Plant-Microbe Interactions and Immunity
- Chromosomal and Genetic Variations
- Virus-based gene therapy research
Howard Hughes Medical Institute
2020-2021
University of California, Davis
2020-2021
Villanova University
2013
Vanderbilt University
1990
Adenovirus E1A transforming function requires two distinct regions of the protein. Transforming activity is closely linked with presence a region designated conserved domain 2 and ability this to bind product cellular retinoblastoma tumor suppressor gene. We have investigated biological properties second E1A, which located near N terminus. Transformation-defective mutants containing deletions in terminus (deletion residues between amino acids 36) were deficient induce DNA synthesis repress...
In vivo assembly of plasmids has become an increasingly used process, as high throughput studies in molecular biology seek to examine gene function. this study, we investigated the plasmid construction technique called gap repair cloning (GRC) two closely related species yeast - Saccharomyces cerevisiae and Candida glabrata. GRC utilizes homologous recombination (HR) activity join a linear vector piece DNA that contains base pair homology. We demonstrate minimum 20 bp homology on each side...
Abstract During meiosis, crossover recombination connects homologous chromosomes to direct their accurate segregation 1 . Defects in crossing over cause infertility, miscarriage and congenital disease. Accordingly, each pair of attains at least one through processes that designate then implement with high efficiency 2 At the DNA level, is implemented formation biased resolution double-Holliday Junction intermediates 3–5 A central tenet two Holliday junctions are resolved opposite planes by...