A5089792071- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Nicotinic Acetylcholine Receptors Study
- Marine Toxins and Detection Methods
- Epilepsy research and treatment
- Cannabis and Cannabinoid Research
- Neurotransmitter Receptor Influence on Behavior
- Neuroscience of respiration and sleep
- MicroRNA in disease regulation
- Pain Mechanisms and Treatments
- RNA Research and Splicing
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Nerve injury and regeneration
- Psychosomatic Disorders and Their Treatments
- Mitochondrial Function and Pathology
- Pulmonary Hypertension Research and Treatments
- Botulinum Toxin and Related Neurological Disorders
- Cardiovascular Issues in Pregnancy
- Metabolomics and Mass Spectrometry Studies
- Neurogenesis and neuroplasticity mechanisms
- Genetic Neurodegenerative Diseases
- Traumatic Brain Injury and Neurovascular Disturbances
- Plant-based Medicinal Research
- Migraine and Headache Studies
Carleton University
2024
University of Calgary
2016
Allen Institute for Brain Science
2016
SickKids Foundation
2014
University of Toronto
2014
Hospital for Sick Children
2014
Institut Universitaire en Santé Mentale de Québec
2013
Simon Fraser University
2003-2010
University of British Columbia
2006-2010
Canada's Michael Smith Genome Sciences Centre
2007
Low-voltage-activated, or T-type, calcium (Ca 2+ ) channels are believed to play an essential role in the generation of absence seizures idiopathic generalized epilepsies (IGEs). We describe a homozygous, missense, single nucleotide (G C) mutation Ca v 3.2 T-type channel gene ( Cacna1h genetic epilepsy rats from Strasbourg (GAERS) model IGE. The GAERS gcm produces arginine proline (R1584P) substitution exon 24 , encoding portion III–IV linker region 3.2. segregates codominantly with number...
Seizures are often followed by sensory, cognitive or motor impairments during the postictal phase that show striking similarity to transient hypoxic/ischemic attacks. Here we seizures result in a severe hypoxic attack confined period. We measured brain oxygenation localized areas from freely-moving rodents and discovered event (pO2 < 10 mmHg) after termination of seizures. This lasted over an hour, is mediated hypoperfusion, generalizes people with epilepsy, attenuated inhibiting...
T-type Ca 2+ channels (T-channels) are involved in the control of neuronal excitability and their gating can be modulated by a variety redox agents. Ascorbate is an endogenous agent that function as both anti- pro-oxidant. Here, we show ascorbate selectively inhibits native v 3.2 T-channels peripheral central neurons, well recombinant heterologously expressed human embryonic kidney 293 cells, initiating metal-catalyzed oxidation specific, metal-binding histidine residue domain 1 channel. Our...
Alternative splicing is known to generate multiple functionally distinct calcium channel variants that exhibit spatial and temporal expression patterns. In humans, naturally occurring mutations in genes encoding pore forming α1-subunits are associated with several severe hereditary disorders although it remains be described whether there exists any relationship between the physiological effects of these splice variation. present study, we systematically compare biophysical three type-1...
Low voltage-activated T-type calcium (Ca) channels contribute to the normal development of heart and are also implicated in pathophysiological states such as cardiac hypertrophy. Functionally distinct Ca channel isoforms can be generated by alternative splicing from each three different genes (Ca(V)3.1, Ca(V)3.2,Ca(V)3.3), although it remains described whether specific splice variants associated with developmental pathological conditions. We aimed identify functionally characterize Ca(V)3.2...
Neuronal precursors produced in the subventricular zone throughout an animal9s life migrate tangentially along rostral migratory stream and, once olfactory bulb (OB), turn to radially bulbar layers, where they differentiate into interneurons. Despite extensive investigations, it has remained largely unknown whether same molecular mechanisms control OB neurogenesis during early postnatal development and adulthood. In this study, we show that extracellular matrix glycoprotein tenascin-R (TNR)...
Abstract: The cannabinoid 1 receptor antagonist AM 251 is known to block the inhibitory effects of endocannabinoids and synthetic agonists on transmitter release through an action at presynaptic receptors in brain. We examined ability inhibit sodium channel‐dependent functions binding [ 3 H]batrachotoxinin A 20‐α‐benzoate channels mouse brain synaptic preparations. Depolarization synaptoneurosomes by channel site 2‐specific neurotoxin veratridine, which abolished tetrodotoxin, was found be...
Background and Purpose The chemotherapy agent oxaliplatin can give rise to oxaliplatin‐induced peripheral neuropathy (OIPN). Here, we investigated whether T‐type calcium channels (Ca v 3) contribute OIPN. Experimental Approach We chronically treated mice with assessed pain responses changes in expression of Ca 3.2 channels. also tested the effects channel blockers on cold sensitivity wild‐type null mice. Key Results Oxaliplatin treatment led mechanical hypersensitivity male female Mechanical...
Abstract It has become generally accepted that presynaptic high voltage–activated N‐type calcium channels located in the spinal dorsal horn are a validated clinical target for therapeutic interventions associated with severe intractable pain. Low (T‐type) play number of critical roles nervous system function, including controlling thalamocortical bursting behaviours and generation spike wave discharges slow sleep patterns. There is growing body evidence T‐type also contribute several ways to...
Manipulation of gene expression via recombinant viral vectors and creation transgenic knock-out/in animals has revolutionized our understanding genes that play critical roles during neuronal development pathophysiology neurological disorders. Recently, target-specific genetic manipulations are made possible to perform in combination with specific Cre-lines, albeit costly, labor-intensive time consuming. Thus, alternative methods address important biological questions highly desirable. In...
ABSTRACT Chronic pain is a highly debilitating condition that differs by type, prevalence, and severity between men women. To uncover the molecular underpinnings of these differences, it critical to analyze transcriptomes spinal cord pain‐processing networks for both sexes. Despite several recently published single‐nucleus RNA‐sequencing (snRNA‐seq) studies on function composition mouse cord, gene expression analysis investigating differences males females has yet be performed. Here, we...