A novel Gram-stain-negative strain, designated ZYY5T, was isolated from rice roots. Results of 16S rRNA gene analysis indicated that strain ZYY5T a member the genus Dickeya, with highest similarity to Dickeya zeae DSM 18068T (98.5%). The major fatty acids were summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C16:0 and 8 (C18:1 C18:1 ω6c). Multi-locus sequence using five concatenated genes (16S rRNA, atpD, infB, recA gyrB) phylogenomic based on 2940 core sequences showed formed robust cluster...

Comamonas sp. strain CNB-1, isolated from activated sludge and having a strong ability to degrade 4-chloronitrobenzene (4CNB), was applied for rhizoremediation of 4CNB-polluted soil through association with alfalfa. Confocal laser scanning microscopy revealed that CNB-1 successfully colonized alfalfa roots. Determination populations by cultivation method quantitative competitive PCR technique targeting the chloronitrobenzene nitroreductase gene showed population in rhizosphere about 10-100...

Tree peony (Paeonia suffruticosa) is a perennial plant indigenous to China known for its elegant and vibrantly colorful flowers. A few genes involved in petal pigmentation have been cloned tree peony. However, date, there studies on the comparison selection of stable reference gene expression analysis by quantitative reverse-transcription PCR (qRT-PCR) this species. In study, 10 candidate were evaluated normalization qRT-PCR three cultivars. GAPDH UBC identified as top two most 'Feng Dan'...

Abstract Background The development of plant gene transfer systems has allowed for the introgression alien genes into genomes novel disease control strategies, thus providing a mechanism broadening genetic resources available to breeders. Using tools engineering, broad-spectrum antimicrobial was tested resistance against head blight caused by Fusarium graminearum Schwabe, devastating wheat ( Triticum aestivum L.) and barley Hordeum vulgare that reduces both grain yield quality. Results A...

Tree peony (Paeonia sect. Moutan) has long been known as a horticultural and medicinal plant in China. Recent studies have shown that tree may further potential applications the field of edible oils. The selection cultivation acceptable varieties is key issue. Seed from 21 genotypes belonging to P. ostii, rockii, ludlowii were evaluated for their oil contents fatty acid compositions. results indicated average 28.05%, 30.52%, 27.62% (w/w), respectively. Averaged over all genotypes, major...

Abstract Eight wild species in Sect. Moutan DC (tree peony) of the genus Paeonia grown natural habitats and 1 cultivated specie were investigated to analyze their fatty acid bioactive phenolic compound profiles. For composition, P. ludlowii contained lowest α‐linolenic (27.68%) jishanensis highest (51.96 %) content 9 species. compounds, qiui resveratrol (2.12 mg/g), delavayi β‐gentiobiosylpaeoniflorin (26.23 ostii paeoniflorin (23.66 mg/g). was selected perform a feasibility study because...

Background The rapid expansion of urbanization leads to significant losses soil ecological functions. Microbes directly participate in key processes and play crucial roles maintaining However, we still have a limited understanding underlying mechanisms shaping microbial communities the interactions among taxa park soils. Methods In this study, community variations bacteria fungi urban suburban soils were investigated Shanghai, China. Real-time PCR high-throughput Illumina sequencing used...

Soil organic matter (SOM) plays an important role in the field of climate change and terrestrial ecosystems. SOM large areas, especially urban is difficult to monitor estimate by traditional methods. Urban land structure complex, soil a mixture inorganic constituents with different physical chemical properties. Previous studies showed that remote sensing techniques provide diverse data visible-near-infrared (VNIR)-shortwave infrared (SWIR) spectral range, are promising prediction content on...

A total of 985 bacterial strains with different colony characteristics were isolated from the root tree peony plants (variety 'Fengdan' and 'Lan Furong'); 69 operational taxonomic units identified by amplified ribosomal DNA restriction analysis. Representatives each group selected for partial 16S rRNA gene sequencing phylogenetic The major groups in bulk soil, rhizosphere, rhizoplane Fengdan Firmicutes (63.2%), Actinobacteria (36.3%), Betaproteobacteria (53.0%), respectively. bacteria Lan...

Abstract Soil degradation has been found in urban forests Shanghai, especially the pure plantations. Mixed plantations are considered to improve soil quality because they can stimulate organic matter cycling and increase carbon nutrient content. Although microbes play crucial roles regulating biogeochemical processes, little is known about how mixed affect microbial communities, including bacteria, archaea, fungi. Here, we evaluated chemical properties, abundances compositions of bacterial,...

Accurate elimination of environmental variability on bridge modal frequency is a prerequisite for high-quality structural performance evaluation. However, the non-Gaussian and nonlinear characteristics data distribution associated with variable environments restrict application anomaly warning methods inaccurate or unreliable detection results. Consequently, an early method in abnormal based localized principal component differences model through integrating slow feature analysis (SFA)...

A Gram-negative, rod-shaped aerobic bacterium designated as strain 2R12T was isolated from the rhizosphere soil of Hosta plantaginea. Phylogenetic analyses based on 16S rRNA gene revealed that should be assigned to genus Chitinophaga with highest sequence similarity arvensicola DSM 3695T (99.1 %) and ginsengisegetis 18108T (98.6 %). The major fatty acids (>10 were iso-C15 : 0, C16 :1ω5c iso-C17 0 3-OH. polar lipids phosphatidylethanolamine, two unidentified aminolipids five lipids....

Purpose During service, cracks are caused in prestressed concrete beams owing to overload or other non-load factors. These significantly affect the safety of bridge structures. The purpose this paper is carry out a non-linear iterative calculation for section beam and obtain change stiffness after cracks. Design/methodology/approach existing stress reinforcement was measured by performing boring release test on two pieces an in-service 16 m hollow plate. Considering effects materials, model...