A5066571270- Sepsis Diagnosis and Treatment
- Intensive Care Unit Cognitive Disorders
- Drug-Induced Hepatotoxicity and Protection
- Liver Disease Diagnosis and Treatment
- Mechanical Circulatory Support Devices
- Cancer, Lipids, and Metabolism
- Pancreatic and Hepatic Oncology Research
- Liver Disease and Transplantation
- Drug Transport and Resistance Mechanisms
- 3D Printing in Biomedical Research
- Pharmacogenetics and Drug Metabolism
- Chronic Lymphocytic Leukemia Research
- Antibiotics Pharmacokinetics and Efficacy
- Antifungal resistance and susceptibility
- Blood disorders and treatments
- Respiratory Support and Mechanisms
- Pesticide Exposure and Toxicity
- Viral Infectious Diseases and Gene Expression in Insects
- Barrier Structure and Function Studies
- Advanced Drug Delivery Systems
- Anesthesia and Sedative Agents
- Neurological Disease Mechanisms and Treatments
- Alcohol Consumption and Health Effects
- Plant Toxicity and Pharmacological Properties
- Medical Device Sterilization and Disinfection
Fraunhofer Institute for Cell Therapy and Immunology
2015-2025
University of Rostock
2017-2020
hiPSC-derived blood-brain barrier (BBB) models are valuable for pharmacological and physiological studies, yet their translational potential is limited due to insufficient cell phenotypes the neglection of complex environment BBB. This study evaluates plasma compatibility with microvascular endothelial-like cells enhance in vitro BBB models. Therefore, samples (sodium/lithium heparin, citrate, EDTA) serum from healthy donors were tested on at concentrations 100%, 75%, 50%. After 24 h,...
Immunoadsorption (IA) has proven to be clinically effective in the treatment of steroid-refractory multiple sclerosis (MS) relapses, but its mechanism action remains unclear. We used miniaturized adsorber devices with a tryptophan-immobilized polyvinyl alcohol (PVA) gel sorbent mimic IA patients MS vitro. The plasma was screened before and after adsorption regard disease-specific mediators, effect on migration neutrophils integrity endothelial cell barrier tested cell-based models. vitro...
Purpose. Drug-induced liver injury (DILI) is the most common cause of and a serious clinical problem; antimycotics are involved in approximately 3% all DILI cases. The hepatotoxicity many drugs, including antimycotics, poorly screened human models. Methods. In standardized assay cytotoxicity on hepatocytes different concentrations (Cmax, 5x Cmax, 10x Cmax) used for systemic infections was tested. Anidulafungin (ANI), liposomal amphotericerin B (L-AmB), caspofungin (CASPO), fluconazole...
Purpose. Liver dysfunction and failure are severe complications of sepsis result in poor outcome increased mortality. The underlying pathologic mechanisms hepatocyte necrosis during only incompletely understood. Here, we investigated whether procalcitonin, a biomarker sepsis, modulates liver cell function viability. Materials Methods. Employing previously characterized patented biosensor system evaluating toxicity vitro, human hepatocellular carcinoma cells (HepG2/C3A) were exposed to...
Purpose. Granulocyte transfusions have been used to treat immune cell dysfunction in sepsis. A granulocyte bioreactor for the extracorporeal treatment of sepsis was tested a prospective clinical study focusing on dosage norepinephrine patients and influence dynamic based liver tests during therapies. Methods Patients. Ten with severe were treated twice within 72 h system containing granulocytes from healthy donors. Survival, physiologic parameters, extended hemodynamic measurement,...
Severe hepatosplenic injury of mansonian schistosomiasis is caused by Th2 mediated granulomatous response against parasite eggs entrapped within the periportal tissue. Subsequent fibrotic scarring and deformation/sclerosing intrahepatic portal veins lead to hypertension, ascites, oesophageal varices. The murine model Schistosoma mansoni (S. mansoni) infection suitable establish severe disease a reasonable time scale for development novel antifibrotic or anti-infective strategies S....
Immune cell dysfunction is a central part of immune paralysis in sepsis. Granulocyte concentrate (GC) transfusions can induce tissue damage via local effects neutrophils. The hypothesis an extracorporeal plasma treatment with granulocytes to show beneficial fewer side effects. Clinical trials standard GC have supported this approach. This ex vivo study investigated the functional properties purified granulocyte preparations during treatment.Purified were stored for up 3 days and compared...
Liver dysfunction (LD) and liver failure are associated with poor outcome in critically ill patients. In patients severe sepsis or septic shock, LD occurred nearly 19% of An early diagnosis at time initial damage the can lead to a better prognosis these because an start therapy is possible. We performed second prospective study test new cell-based cytotoxicity device (biosensor) evaluate clinical relevance for prognostic capacity. study, 99 intensive care unit were included two groups. From...
Albumin is the most abundant plasma protein and functions as a transport molecule that continuously interacts with various cell types. Because of these properties, albumin has been exploited by pharmaceutical industry to improve drug delivery into target cells. The immediate effects on cells, however, require further understanding. interacting properties applications incentivises continual research HepG2/C3A hepatocellular carcinoma line used model for studying cancer pathology well liver...
Abstract Background Use of donor granulocyte concentrate (GC) has been limited due to its short storage time 6–24 h, which is partially residual red blood cells (RBCs) and platelets the resulting lactate production leading an acidotic milieu. To increase this time, we developed a closed system procedure compatible with standard bank technologies remove RBC enrich GC. Methods Standard GCs (sGCs) were sedimented, washed twice 0.9% sodium chloride (NaCl), resuspended in group‐identical fresh...
Abstract A granulocyte bioreactor for the extracorporeal treatment was developed to enhance immune cell function in patients with severe sepsis. The influence of oxygenation on used cells tested a prospective clinical study. Ten sepsis were treated twice bioreactor. screened functionality; values blood gases, glucose and lactate obtained from recirculating circuit. Five an oxygenator setup (Oxy group), five without (Non‐Oxy group). overall in‐hospital mortality 50%. Significantly lower...
Immune cell dysfunction plays a central role in sepsis-associated immune paralysis. The transfusion of healthy donor cells, i.e., granulocyte concentrates (GC) potentially induces tissue damage via local effects neutrophils. Initial clinical trials using standard GC strictly extracorporeal bioreactor system for treatment septic shock patients already provided evidence beneficial with fewer side effects, by separating patient and cells plasma filters. In this ex vivo study, we demonstrate the...
Granulocyte concentrates (GCs) are usually prepared by single-donor apheresis after G-CSF pretreatment and have to be transfused within 24 h cell collection because of the rapid decrease in pH survival due high lactate production red blood contamination. GCs pooled from buffy coats whole donations could improve availability these products. Methods reduce platelet contamination may storability. We developed a manufacturing process for investigated viability functionality over time.
Recent research has concentrated on the development of suitable in vitro cell models for early identification hepatotoxicity during drug order to reduce number animal and obtain a better predictability hepatotoxic reactions humans. The aim presented study was identify translational biomarkers acute liver injury human patients that can serve as hepatocellular vivo simple models. Therefore, 188 different metabolites from with acute-on-chronic failure before after transplantation were analyzed...
Albumin is the most abundant plasma protein and functions as a transport molecule that continuously interacts with various cell types. Because of these properties, albumin has been exploited by pharmaceutical industry to improve drug delivery target cells. Understanding immediate effects on cells however limited. The interacting properties applications incentivises continual research into HepG2/C3A hepatocellular carcinoma line used model for studying cancer pathology well liver biosynthesis...
Albumin is the most abundant plasma protein and functions as a transport molecule that continuously interacts with various cell types. Because of these properties, albumin has been exploited by pharmaceutical industry to improve drug delivery target cells. Understanding immediate effects on cells however limited. The interacting properties applications incentivises continual research into HepG2/C3A hepatocellular carcinoma line used model for studying cancer pathology well liver biosynthesis...