A5037990495- Connexins and lens biology
- Liver Diseases and Immunity
- Drug-Induced Hepatotoxicity and Protection
- Heat shock proteins research
- Liver Disease Diagnosis and Treatment
- CAR-T cell therapy research
- Pediatric Hepatobiliary Diseases and Treatments
- Immunotherapy and Immune Responses
- Autoimmune Neurological Disorders and Treatments
- Advanced Biosensing Techniques and Applications
- interferon and immune responses
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nicotinic Acetylcholine Receptors Study
- Carcinogens and Genotoxicity Assessment
- Hepatitis B Virus Studies
- Animal testing and alternatives
- RNA regulation and disease
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Smoking Behavior and Cessation
- Thyroid Disorders and Treatments
- Bacillus and Francisella bacterial research
- Alcohol Consumption and Health Effects
- Neuroscience and Neuropharmacology Research
- Drug Transport and Resistance Mechanisms
Vrije Universiteit Brussel
2020-2021
Centre National de la Recherche Scientifique
2019
Institut NeuroMyoGène
2019
Inserm
2019
Université Claude Bernard Lyon 1
2019
Connexins are goal keepers of tissue homeostasis, including in the liver. As a result, they frequently involved disease. The current study was set up to investigate effects cholestatic disease on production connexin26, connexin32 and connexin43 For this purpose, bile duct ligation, well-known trigger liver injury, applied mice. In parallel, human hepatoma HepaRG cell cultures were exposed drugs acids. Samples from both vivo vitro settings subsequently subjected assessment mRNA protein...
Drug-induced liver injury, including cholestasis, is an important clinical issue and economic burden for pharmaceutical industry healthcare systems. However, human-relevant in vitro information on the ability of other types chemicals to induce cholestatic hepatotoxicity lacking. This work aimed at investigating potential non-pharmaceutical using primary human hepatocytes cultured 3D spheroids. Spheroid cultures were repeatedly (co-) exposed drugs (cyclosporine-A, bosentan, macitentan) or...
Liver cancer cell lines are frequently used in vitro tools to test candidate anti-cancer agents as well elucidate mechanisms of liver carcinogenesis. Among such is cellular communication mediated by connexin-based gap junctions. The present study investigated changes connexin expression and junction functionality vitro. For this purpose, seven human lines, primary hepatocytes, were subjected analysis at the transcriptional, translational activity level. Real-time quantitative reverse...
Connexin-based channels play key roles in cellular communication and can be affected by deleterious chemicals. In this study, the effects of various genotoxic carcinogenic compounds, non-genotoxic compounds non-carcinogenic on expression functionality connexin-based channels, both gap junctions connexin hemichannels, were investigated human hepatoma HepaRG cell cultures. Expression connexin26, connexin32, connexin43 was evaluated means real-time reverse transcription quantitative polymerase...
Abstract Bispecifics targeting multiple antigens or epitopes have been showing great promise for treatment of cancer. However, managing unwanted immunogenicity has become a challenge in the development cycle these promising therapeutics as there is trend towards higher immune responses compared with classical monoclonal antibodies. In vitro assays using human primary cells can be used to assess risk induction cytokine release storm immunogenicity. For latter, T cell activation and...
Abstract Neutrophils have shown a lot of plasticity with important consequences on cancer disease progression and been associated multiple functions such as killing antibody-opsonized cells, directly kill tumor cells through the release reactive oxygen nitrogen species controversial role in micro-environment both pro- anti-tumor roles. Studying neutrophil function vitro can be done using Incucyte® live imaging system to monitor antibody-dependent cell-mediated cytotoxicity (ADCC). Optimised...
Abstract The increasing interest in the tumor microenvironment leads to a focus on new bioassays represent all players of cancer immune response. Some these like regulatory T cells play an important role by downregulating anti-tumor Their regulation mechanisms constitute target for therapeutics. In order study human model, suppressive Treg mimicking action were developed and optimized. vitro assays come with many technical challenges, therefore protocols purification culture optimized...