A5010159317- Drug-Induced Hepatotoxicity and Protection
- Drug Transport and Resistance Mechanisms
- Liver Disease Diagnosis and Treatment
- Liver Diseases and Immunity
- Liver physiology and pathology
- Pharmacological Effects and Toxicity Studies
- Connexins and lens biology
- Hepatitis B Virus Studies
- Pediatric Hepatobiliary Diseases and Treatments
- Dye analysis and toxicity
- Pancreatic function and diabetes
- Biochemical Analysis and Sensing Techniques
- Heme Oxygenase-1 and Carbon Monoxide
- Liver Disease and Transplantation
- Pancreatitis Pathology and Treatment
- Cancer-related gene regulation
- Immune cells in cancer
- RNA regulation and disease
- Nephrotoxicity and Medicinal Plants
- Kruppel-like factors research
- Pesticide Exposure and Toxicity
- Cardiovascular Disease and Adiposity
- Metabolomics and Mass Spectrometry Studies
- Fibroblast Growth Factor Research
- Pharmacogenetics and Drug Metabolism
Vrije Universiteit Brussel
2017-2023
Ghent University
2021-2023
Pharmac
2018
Background and Aims: Primary sclerosing cholangitis (PSC) is an immune-mediated cholestatic liver disease for which pharmacological treatment options are currently unavailable. PSC strongly associated with colitis a disruption of the gut-liver axis, macrophages involved in pathogenesis PSC. However, how interactions specific macrophage populations contribute to incompletely understood. Approach Results: We investigated impact cholestasis on hepatic colonic microenvironment, performed...
A frequent side effect of many drugs includes the occurrence cholestatic liver toxicity. Over past couple decades, drug-induced cholestasis has gained considerable attention, resulting in a plethora data regarding its prevalence and mechanistic basis. Likewise, several food additives dietary supplements have been reported to cause insults few years. The induction hepatotoxicity by other types chemicals, particular synthetic compounds, such as industrial biocides, cosmetic ingredients, much...
Abstract Due to a combination of rapid disease progression and the lack curative treatment options, hepatocellular carcinoma (HCC) is one deadliest cancers worldwide. Infiltrated, monocyte‐derived, tumor‐associated macrophages are known play role in HCC pathogenesis, but involvement Kupffer cells (KCs) remains elusive. Here, we used Clec4F‐diphteria toxin receptor transgenic mouse model specifically investigate effect KC depletion on initiation, neoplastic growth following liver resection....
Drug-induced liver injury, including cholestasis, is an important clinical issue and economic burden for pharmaceutical industry healthcare systems. However, human-relevant in vitro information on the ability of other types chemicals to induce cholestatic hepatotoxicity lacking. This work aimed at investigating potential non-pharmaceutical using primary human hepatocytes cultured 3D spheroids. Spheroid cultures were repeatedly (co-) exposed drugs (cyclosporine-A, bosentan, macitentan) or...
Connexins are goal keepers of tissue homeostasis, including in the liver. As a result, they frequently involved disease. The current study was set up to investigate effects cholestatic disease on production connexin26, connexin32 and connexin43 For this purpose, bile duct ligation, well-known trigger liver injury, applied mice. In parallel, human hepatoma HepaRG cell cultures were exposed drugs acids. Samples from both vivo vitro settings subsequently subjected assessment mRNA protein...
Adherens junctions, consisting of cadherins and catenins, are a group cell-to-cell junctions that mediate mechanistic linkage between neighboring cells. By doing so, adherens ensure direct intercellular contact play an indispensable role in maintaining tissue architecture. Considering these critical functions, it is not surprising frequently involved disease. In the present study, effects bile duct ligation—a surgical procedure to experimentally induce cholestatic fibrotic liver pathology—on...
The transcriptomic dataset (whole genome microarray Affymetrix Human U133 plus 2.0 and Mouse Genome 430 2.0) presented in this paper describes the differential gene expression profile of a human vitro model drug-induced cholestasis well-known mouse vivo cholestasis. consists hepatoma HepaRG cells monolayer configuration exposed to 3 different cholestatic drugs with or without bile acids. For modelling cholestasis, mice were subjected duct ligation surgery. Consecutive normalization,...
Connexin proteins can form hexameric hemichannels and gap junctions that mediate paracrine direct intercellular communication, respectively. Gap junction activity is crucial for the maintenance of hepatic homeostasis, while connexin become particularly active in liver disease, such as hepatitis, fibrosis, cholestasis or even hepatocellular carcinoma. Channels consisting connexin-like named pannexins have been directly linked to inflammation cell death. The goal present study was characterize...
Systematic comparison of experimental and human obstructive cholestasis reveals conservation canonical