A5049533111- Eicosanoids and Hypertension Pharmacology
- Retinoids in leukemia and cellular processes
- Pesticide Exposure and Toxicity
- Pesticide and Herbicide Environmental Studies
- Diet and metabolism studies
- Ubiquitin and proteasome pathways
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Nutrition and Health in Aging
- Inflammatory mediators and NSAID effects
- Ion Transport and Channel Regulation
- Peptidase Inhibition and Analysis
- Alcohol Consumption and Health Effects
- Cardiac Ischemia and Reperfusion
- Animal testing and alternatives
- Hepatitis C virus research
- Genetic Neurodegenerative Diseases
- Receptor Mechanisms and Signaling
- Congenital Heart Disease Studies
- Muscle metabolism and nutrition
- Pharmacogenetics and Drug Metabolism
- Anesthesia and Neurotoxicity Research
- Biological Research and Disease Studies
- Muscle Physiology and Disorders
- Allelopathy and phytotoxic interactions
Children's Hospital of Philadelphia
2023-2025
University of California, Davis
2018-2024
Antibodies are one of the most used reagents in scientific laboratories and critical components for a multitude experiments physiology research. Over past decade, concerns about many biological methods, including those that use antibodies, have arisen as several were unable to reproduce data obtained other laboratories. The lack reproducibility could be largely attributed inadequate reporting detailed no or limited verification by authors, production unvalidated antibodies. goal this...
Background The Fontan operation is the current standard of care for single‐ventricle congenital heart disease. Almost all patients with develop liver fibrosis at a young age, known as Fontan‐associated disease (FALD). pathogenesis and mechanisms underlying FALD remain little understood, there are no effective therapies. We aimed to present comprehensive multiomic analysis human FALD, revealing fundamental biology FALD. Methods Results recently generated single‐cell transcriptomic epigenomic...
Cancer-associated cachexia (CAC) is a critical contributor to pancreatic ductal adenocarcinoma (PDAC) mortality. Thus, there an urgent need for new strategies mitigate PDAC-associated cachexia; and the exploration of dietary interventions component. We previously observed that ketogenic diet (KD) combined with gemcitabine enhances overall survival in autochthonous LSL-KrasG12D/+; LSL-Trp53 R172H/+; Pdx1-Cre (KPC) mouse model. In this study, we investigated effect cellular mechanisms KD...
Abstract The tumor suppressor p53 is thought to play a key role in the maintenance of cell size and homeostasis, but relatively little known about its skeletal muscle. Based on ability suppress growth, we hypothesized that inhibiting function wild-type through overexpression dominant-negative mutant (DDp53) could result muscle fiber hypertrophy. To test this hypothesis, electroporated adult rat tibialis anterior muscles with DDp53 collected tissue three weeks later. We confirmed successful...
Cancer associated cachexia (CAC) is a critical contributor to pancreatic ductal adenocarcinoma (PDAC) mortality. Thus, there an urgent need for new strategies mitigate PDAC-associated cachexia; and the exploration of dietary interventions component. We previously observed that ketogenic diet (KD) combined with gemcitabine enhances overall survival in autochthonous LSL-KrasG12D/+; LSL-Trp53 R172H/+; Pdx1-Cre (KPC) mouse model. In this study, we investigated effect cellular mechanisms KD...
Prometryn is a methylthio-s-triazine herbicide used to control the growth of annual broadleaf and grass weeds in many cultivated plants. Significant traces prometryn are documented environment, mainly waters, soil, plants for human domestic consumption. Previous studies have shown that triazine herbicides carcinogenic potential humans. However, there limited information about effects on cardiac system literature, or mechanisms signaling pathways underlying any cytotoxic not known. It...
Pesticides are important biological agents that deter or kill pests. Globally, about 2 million tons of pesticides being utilized each year which highlights their importance. Previous studies have suggested high levels pesticide exposure associated with increased risk chronic diseases, including cancers, cardiotoxicity, Parkinson's disease, diabetes mellitus, neurological deficits, birth defects, and reproductive disorders. Prometryn is a commonly used selective triazine herbicide, currently...
Ibuprofen, a common over-the-counter drug, is taken worldwide to alleviate pain and reduce inflammation. While the recommended dosage generally regarded as safe for consumption, complications arise when drug chronically, even in prescribed quantities. Previous research suggests that ibuprofen alters productivity of various signal transduction pathways throughout body stimulates production reactive oxygen species (ROS) liver tissue. Our aimed determine if proteins involved proteolysis energy...
Oxylipins are a group of bioactive fatty acid metabolites generated via enzymatic oxygenation. They notably involved in inflammation, pain, vascular tone, hemostasis, thrombosis, immunity, and coagulation. have become the focus therapeutic intervention since they implicated many conditions, such as nonalcoholic liver disease, cardiovascular aging. The plays crucial role lipid metabolism distribution throughout organism. Long-term exposure to pesticides is suspected contribute hepatic...
Introduction: Congenital heart diseases (CHDs) are the most common birth defects in U.S. There growing numbers of infants and children living with single ventricle congenital disease (SVCHD), a unique cardiac anomaly which there is only one functional ventricle. The Fontan surgery current standard care for SVCHD. Although operative survival has improved over years, it now evident that these surviving patients facing new life-threatening challenges: severe complications resulting from...
Background and Objective Cardiovascular disease (CVD) is the leading cause of death a major disability worldwide. Previous studies have shown that long‐term chronic use nonsteroidal anti‐inflammatory drugs (NSAIDs) increases risk developing stroke cardiovascular diseases. Naproxen an FDA‐approved NSAID currently used to treat several medical conditions such as pain, osteoarthritis, rheumatoid arthritis, polyarticular juvenile idiopathic tendonitis, etc. Acetaminophen, active ingredient in...
Ibuprofen is one of the most commonly used compounds in treatment pain, fever, and inflammation. Despite its beneficial effects, long-term use ibuprofen has been previously associated with increased risks for cardiovascular diseases all-cause mortality. Relatively little known about signaling pathways or mechanism(s) involved ibuprofen’s ability to increase risk diseases. Very also sex-related differences respect ibuprofen. We investigated effects (100 mg/kg/day, equivalent a 500mg...
Acetaminophen (APAP) is a common over-the-counter medication used to treat pain and fever. It can be taken orally, topically, or intravenously considered safe effective drug when at therapeutic doses. However, recent research suggests that taking APAP regularly result in increased blood pressure cardiovascular risk. Previous publications found Non-Steroidal-Anti-Inflammatory-Drugs (NSAIDs) Reactive Oxygen Species (ROS) cardiomyocytes, resulting cardiac dysfunction. Although not NSAID, likely...
Introduction and objectives Previous clinical studies have suggested that nonsteroidal anti‐inflammatory drugs (NSAIDs) are associated with increased risk of stroke cardiovascular disease. Acetaminophen, the active ingredient in Tylenol, is not an NSAID liver toxicity. The common use NSAIDs for pain fever relief a concern but it unclear if also causing adverse effects on other tissues such as liver, kidney brain. research our laboratory has cause proteasome dysfunction cardiac tissue. To...
Western Blotting, which is probably better referred to as immunoblotting, one of the most commonly used biological methods worldwide. This technique capable detecting an individual protein from a complex mixture proteins extracted cells or tissues. The major steps in Blotting workflow are 1) separation based on size, 2) transfer separated suitable stable support, 3) Interaction between target and appropriate primary antibodies. In many cases, secondary antibody that interacts with used, 4)...