A5027103185- Nematode management and characterization studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Reproductive Biology
- Insect symbiosis and bacterial influences
- Enzyme Production and Characterization
- Plant Virus Research Studies
- Banana Cultivation and Research
- Cassava research and cyanide
- Insect-Plant Interactions and Control
- Phytochemistry Medicinal Plant Applications
- Plant Molecular Biology Research
- Phytoplasmas and Hemiptera pathogens
- Dye analysis and toxicity
- Plant nutrient uptake and metabolism
- Diatoms and Algae Research
- Plant Pathogenic Bacteria Studies
- Pesticide and Herbicide Environmental Studies
- Food Safety and Hygiene
- Agricultural and Food Sciences
- Mosquito-borne diseases and control
- Entomopathogenic Microorganisms in Pest Control
- Microbial Metabolism and Applications
Washington State University
2025
University of California, Riverside
2016-2023
Universidade de Brasília
2009
Summary Root‐knot nematodes (RKNs; Meloidogyne spp.) are plant parasites with a broad host range causing great losses worldwide. To parasitize their hosts, RKNs establish feeding sites in roots known as giant cells. The majority of work studying plant–RKN interactions susceptible hosts addresses establishment the cells and there is limited information on early defense responses. Here we characterized or pattern‐triggered immunity (PTI) against Arabidopsis thaliana . address PTI, evaluated...
We demonstrated previously that expression of Macrosiphum euphorbiae salivary protein Me10 enhanced aphid reproduction on its host tomato (Solanum lycopersicum). However, the mechanism action remained elusive. To confirm secretion by into plant tissues, we produced polyclonal antibodies. identify targets Me10, developed a immune induced complementary DNA yeast two-hybrid library and screened it with as bait. Immunoprecipitation bimolecular fluorescence complementation (BiFC) assays were...
Plant-parasitic nematodes (PPNs) cause billions of dollars in agricultural losses annually. In the United States (U.S.), a well-established list prevalent serves as foundation for addressing known threats. However, climate change is expected to trigger significant shifts nematode populations, behaviors, and host ranges, introducing new risks ecosystems. Understanding how adapt evolving environments crucial predicting their spread locations hosts. Beyond current PPN there ongoing threat...
It is hypothesized that pathogens use expansin-like effectors due to their cell wall extension activity (Liu et al., 2016), manipulate or mimic the host's biological processes, modulate such as root attachment (Tancos 2018).
The root-knot nematode (RKN) Meloidogyne chitwoodi is a threat for potato production in the western United States, negatively impacting yield and product value. produce proteins, called effectors, their esophageal glands that are secreted during parasitism play integral roles plant-nematode interactions. Because main effector secretory organ, we performed juvenile gland isolation transcriptome analysis. data allowed us to improve M. genome annotation. Additionally, gland-specific gave an...
Pathogen perception by plants is mediated plasma membrane-localized immune receptors that have varied extracellular domains. Lectin receptor kinases (LecRKs) are among these and subdivided into 3 classes, C-type LecRKs (C-LecRKs), L-type (L-LecRKs) G-type (G-LecRKs). While C-LecRKs represented one or two members in all plant species investigated unknown functions, L-LecRKs been characterized a few shown to play roles defense against pathogens. Whereas Arabidopsis G-LecRKs characterized, this...
Root-knot nematodes (Meloidogyne spp., RKN) are responsible for extensive crop losses worldwide. During infection, they penetrate plant roots, migrate between cells, and establish feeding sites, known as giant near the root vasculature. Previously, we found that nematode perception early responses in plants were similar to those of microbial pathogens required BRI1-ASSOCIATED KINASE1/SOMATIC EMBRYOGENESIS RECEPTOR KINASE3 (BAK1/SERK3) coreceptor Arabidopsis (Arabidopsis thaliana) tomato...
A virus with a large genome was identified in the transcriptome of potato aphid (Macrosiphum euphorbiae) and named Macrosiphum euphorbiae 1 (MeV-1). The MeV-1 is 22 780 nt size, including 3' 5' non-coding regions, single ORF encoding putative polyprotein 7333 aa. C-terminal region predicted contained sequences similarities to helicase, methyltransferase RNA-dependent RNA polymerase (RdRp) motifs, while N-terminal lacked any motifs structural proteins. Phylogenetic analysis helicase placed...
The potato aphid, Macrosiphum euphorbiae, is an important agricultural pest that causes economic losses to and tomato production. To establish the transcriptome for this RNA-Seq libraries constructed from aphids maintained on plants were used in Illumina sequencing generating 52.6 million 75–105 bp paired-end reads. reads assembled using Velvet/Oases software with SEED preprocessing resulting 22,137 contigs N50 value of 2,003bp. After removal host origin, 20,254 annotated BLASTx searches...
Abstract Root-knot nematodes ( Meloidogyne spp., RKN) are responsible for extensive crop losses worldwide. For infection, they penetrate plant roots, migrate between cells, and establish feeding sites, known as giant in the root pericycle. Previously, we found that nematode perception early responses were similar to those microbial pathogens require BAK1 co-receptor Arabidopsis thaliana tomato. To identify additional receptors involved this process, implemented a reverse genetic screen...