A5088973419- Cell Adhesion Molecules Research
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Platelet Disorders and Treatments
- CAR-T cell therapy research
- Liver Disease Diagnosis and Treatment
- Hepatitis C virus research
- Cancer Immunotherapy and Biomarkers
- Hepatitis B Virus Studies
- Cellular Mechanics and Interactions
- Glycosylation and Glycoproteins Research
- Sodium Intake and Health
- HER2/EGFR in Cancer Research
- Phagocytosis and Immune Regulation
- Virus-based gene therapy research
- Chemokine receptors and signaling
- Gallbladder and Bile Duct Disorders
- Pain Mechanisms and Treatments
- NF-κB Signaling Pathways
- Blood Pressure and Hypertension Studies
- Protein Kinase Regulation and GTPase Signaling
- Neuropeptides and Animal Physiology
University of Minnesota Medical Center
2009-2023
University of Minnesota
2013-2022
University of Minnesota System
2002-2019
Masonic Cancer Center
2016-2019
Institute of Immunology
1999-2019
Stanford University
2019
Shanghai Tenth People's Hospital
2016
Tongji University
2016
University of Graz
2016
Saint Louis University
2016
Abstract Functional studies demonstrate that T cell activation often requires not only occupancy of the TCR but costimulatory interactions other molecules, which remain largely undefined. We have tested hypothesis LFA-1 interaction with its ligand intercellular adhesion molecule 1 (CD54) (ICAM-1) is such a in model system using biochemically purified ICAM-1 and cross-linking by anti-CD3 mAb OKT3 immobilized on plastic. Resting cells do respond to However deposited plastic together...
Given prior evidence that adhesion molecules play critical roles in T cell recognition, it is important to identify new pathways and explore their role activation. Our studies of proliferation complement concurrent adhesion; both demonstrate resting CD4+ human lymphocytes express the VLA integrins VLA-4, VLA-5, VLA-6, can use these receptors interact with extracellular matrix (ECM) proteins fibronectin (VLA-4 VLA-5) laminin (VLA-6). VLA-dependent interaction cells (FN) (LN) facilitates...
The CD31 (platelet endothelial cell adhesion molecule-1 [PECAM-1]/endothelial molecule [endoCAM]) expressed on leukocytes, platelets, and cells is postulated to mediate thereby function in immunity, inflammation, wound healing. We report the following novel features of which suggests a role for it amplification unique T subsets: (a) engagement induces adhesive beta 1 2 integrins; (b) induction by immunoglobulin G (IgG) monoclonal antibodies (mAbs) sensitive, requiring only bivalent mAb; (c)...
Studies of T cell adhesion and activation reveal two new functions the CD44 molecule, a molecule now recognized to be identical three molecules functional interest: Pgp-1, Hermes, extracellular matrix receptor type III (ECMRIII). By screening for mAb which inhibit E, we have identified functionally unique CD44-specific mAb, NIH44-1, partially inhibits rosetting by binding on E. immunoprecipitates protein 85 110 kDa with broad tissue distribution, was determined specific based comparison its...
Ubiquitously expressed β-actin and γ-actin isoforms play critical roles in most cellular processes; however, their unique contributions are not well understood. We generated whole-body β-actin-knockout (Actb(-/-)) mice demonstrated that is required for early embryonic development. Lethality of Actb(-/-) embryos correlated with severe growth impairment migration defects primary mouse fibroblasts (MEFs) were observed γ-actin-null MEFs. Migration associated reduced membrane protrusion dynamics...
T cell adhesion to endothelium is critical lymphocyte recirculation and influx into sites of inflammation. We have systematically analyzed the role four receptor/ligand interactions that mediate peripheral human CD4+ cells cultured umbilical vein endothelial (HUVEC): LFA-1 binding ICAM-1 an alternative ligand ("ICAM-X"), VLA-4 VCAM-1, ELAM-1. Contributions these pathways depend on activation state both HUVEC, differentiation cell. ELAM-1 plays a significant in mediating resting activated...
In the germinal center (GC), B cells are either selected to become memory or eliminated through process of programmed cell death. FDC which intimately associated with GC thought be important in this selection process. Previously, we have shown that LFA-1 (CD11a/CD18)-ICAM-1 (CD54) and VLA-4 (CD49d)-VCAM-1 (CD106) adhesion pathways involved FDC-B interaction. present study, explored whether these adhesive interactions contribute by studying effects on apoptosis cells. Using derived from human...
SLAP-130/Fyb (SLP-76–associated phosphoprotein or Fyn-binding protein; also known as Fyb/Slap) is a hematopoietic-specific adapter, which associates with and modulates function of SH2-containing leukocyte 76 kilodaltons (SLP-76). T cells from mice lacking show markedly impaired proliferation following CD3 engagement. In addition, the cell receptor (TCR) in mutant fails to enhance integrin-dependent adhesion. Although TCR-induced actin polymerization normal, TCR-stimulated clustering integrin...
We detail in this report the characterization of a human B-lymphoblastoid cell line, .221, that does not express endogenous HLA-A, HLA-B, or HLA-C class I Ag due to gamma-ray-induced mutations HLA complex. Mutant .221 is characterized by: 1) complete absence HLA-A,-B,-C mRNA transcripts and alpha-chains, 2) intracellular expression two non-A,-B,-C alpha-chains with an abundance less than equal 1% normal on similar cells. However, transferred genomic genes are expressed as surface amounts...
The monocyte-derived cytokines, tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), are central regulators of the immune response, but physiologic stimuli for their release remain largely undefined. Engagement three monocyte glycoproteins, LFA-3, CD44, CD45, by specific monoclonal antibodies immobilized on plastic induced TNF-α IL-1β release. In addition, was released when LFA-3 bound immobilized, purified CD2, which is its receptor. Thus, a receptor-ligand interaction that mediates...
Many ligands of adhesion molecules mediate costimulation T cell activation. The generality this emerging concept is best determined by using model systems which exploit physiologically relevant ligands. We developed such an "antigen-specific" system for stimulation resting CD4+ human cells the following purified ligands: (a) major histocompatibility complex class II plus superantigen Staphylococcus enterotoxin A, to engage receptor (TCR); (b) proteins vascular molecule 1 (VCAM-1),...
Abstract A MHC class I gene, HLA-6.2, has been identified. Examination of its primary structure indicates that HLA-6.2 is the most divergent member gene family characterized to date. Tissue distribution RNA differs from other genes in highest level detected resting T lymphocytes. After transfer into a null human lymphoblastoid cell line, protein associated with beta 2-microglobulin detected. Analysis HLA deletion mutant lines places between HLA-C and HLA-A loci on short arm chromosome 6....
The HLA-A, -B, and -C class I human histocompatibility antigens the genes that encode them have been isolated characterized. Apparently complete non-HLA-A, B, C identified on HindIII-generated 5.4-kilobase (kb), 6.0-kb, 6.2-kb DNA fragments derived from lymphoblastoid cell line (LCL) 721. We studied expressibility of these by subcloning into nonintegrating pHeBo vector transferring chimeric plasmids mutant LCL 721.221. This was 721 means immunoselections following gamma-ray mutagenesis...
Regulated adhesion enables T cells to migrate through tissue and transiently interact with an endless succession of cells. Monoclonal antibody (mAb) engagement the CD3/T cell receptor (TCR) complex results in a rapid transient augmentation function LFA-1 VLA integrin molecules on human We show this study that mAb crosslinking cell-specific accessory CD7 CD28, or treatment Ca2+ ionophore A23187, induction integrin-mediated three distinct ligands: extracellular matrix protein fibronectin,...