A5012188195- Protein Interaction Studies and Fluorescence Analysis
- Protein Structure and Dynamics
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Hemoglobin structure and function
- Tardigrade Biology and Ecology
- Advanced Fluorescence Microscopy Techniques
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer-related gene regulation
- Spaceflight effects on biology
- Fungal and yeast genetics research
- Biocrusts and Microbial Ecology
- RNA Research and Splicing
- Nitric Oxide and Endothelin Effects
- Caveolin-1 and cellular processes
- Allelopathy and phytotoxic interactions
- Hypothalamic control of reproductive hormones
- Regulation of Appetite and Obesity
- Enzyme Structure and Function
- Ethnobotanical and Medicinal Plants Studies
- Protein Degradation and Inhibitors
- Reproductive biology and impacts on aquatic species
- Cell death mechanisms and regulation
- RNA modifications and cancer
- Lanthanide and Transition Metal Complexes
University of Wyoming
2023-2024
University of Michigan
2019-2023
Indian Institute of Technology Delhi
2015-2020
University of Calcutta
2015
Rashtrasant Tukadoji Maharaj Nagpur University
1993-2014
Indian Institute of Technology Kanpur
2010
Tardigrades are microscopic animals that survive desiccation by inducing biostasis. To drying tardigrades rely on intrinsically disordered CAHS proteins, which also function to prevent perturbations induced in vitro and heterologous systems. proteins have been shown form gels both vivo, has speculated be linked their protective capacity. However, the sequence features mechanisms underlying gel formation necessity of gelation for protection not demonstrated. Here we report a mechanism...
The effect of macromolecular crowding on protein structure and dynamics has mostly been explained the basis excluded volume effect, its origin being entropic. In recent times a progressive shift in this view taking place with increasing emphasis soft interactions that are enthalpic by nature. Using very low concentrations (1–10 g/L) both synthetic (dextran- poly(ethylene glycol) (PEG)-based) (α-synuclein myoglobin)-based crowders, we have shown solvation probe molecule ANS...
In the living cell, biomolecules perform their respective functions in presence of not only one type macromolecules but rather various with different shapes and sizes. this study, we have investigated effects five single macromolecular crowding agents, Dextran 6, 40, 70, Ficoll PEG 8000 binary mixtures on modulation domain separation human serum albumin using a Förster resonance energy transfer-based approach translational mobility small fluorescent probe fluorescein isothiocyanate (FITC)...
HP1 proteins traverse a complex and crowded chromatin landscape to bind with low affinity but high specificity histone H3K9 methylation (H3K9me) form transcriptionally inactive genomic compartments called heterochromatin. Here, we visualize single-molecule dynamics of an homolog, the fission yeast Swi6, in its native environment. By tracking single Swi6 molecules, identify mobility states that map discrete biochemical intermediates. Using mutants perturb H3K9me recognition, oligomerization,...
H3K9 methylation (H3K9me) specifies the establishment and maintenance of transcriptionally silent epigenetic states or heterochromatin. The enzymatic erasure histone modifications is widely assumed to be primary mechanism that reverses silencing. Here, we reveal an inversion this paradigm where a putative demethylase Epe1 in fission yeast, has non-enzymatic function opposes heterochromatin assembly. Mutations within catalytic JmjC domain disrupt its interaction with Swi6HP1 suggesting might...
FRET-based distance changes between domains I and II of HSA reveal significant differences in the manner which dextran based macromolecular crowding agents influence serum protein.
Abstract Tardigrades are microscopic animals that survive desiccation by inducing biostasis. To drying tardigrades rely on intrinsically disordered CAHS proteins form gels. However, the sequence features and mechanisms underlying gel formation necessity of gelation for protection have not been demonstrated. Here we report a mechanism D similar to intermediate filaments. We show restricts molecular motion, immobilizing protecting labile material from harmful effects drying. In vivo , observe...
Protein dynamics in cells have been shown to be markedly different from that dilute solutions because of the highly crowded cellular interior. The volume exclusion arising high concentration macromolecules present can affect both equilibrium and kinetic processes involving protein conformational changes. While global changes structure leading modulations stability well-documented, local a large bearing on functional aspects these biomolecules are rare come across. Using multidomain serum...
Global changes in the conformations of proteins presence macromolecular crowding agents have been well documented. Here, we used solvation dynamics to monitor a specific domain multidomain protein human serum albumin (HSA) various crowders. The probe 6-bromoacetyl-2-dimethylaminonaphthalene was site-specifically attached cysteine-34 I HSA. Analyses time-resolved Stokes shift this revealed significant retardation solvent coordinate, particularly crowder-dependent manner. We attribute observed...
An assessment of plant diversity was carried out to record different species flowering plants (Angiosperms) in Kalyani township Nadia district, West Bengal, India during January, 2014.All together 6 quadrats were laid down, and 30 belonging 15 families documented.Voucher specimens preserved digitized departmental phyto-informatics center.Frequency density varied greatly among the taxa, while many not evenly abundant study area.Out total species, 11 can be used as economic medicinal...
ABSTRACT Visualizing and measuring molecular-scale interactions in living cells represents a major challenge, but recent advances microscopy are bringing us closer to achieving this goal. Single-molecule super-resolution enables high-resolution sensitive imaging of the positions movement molecules cells. HP1 proteins important regulators gene expression because they selectively bind recognize H3K9 methylated (H3K9me) histones form heterochromatin-associated protein complexes that silence...
ABSTRACT HP1 proteins bind with low affinity but high specificity to histone H3 lysine 9 methylation (H3K9me), forming transcriptionally inactive genomic compartments referred as heterochromatin. How traverse a complex and crowded chromatin landscape on the millisecond timescale yet recognize H3K9me remains paradoxical. Here, we visualize single-molecule dynamics of an homolog, fission yeast Swi6, in its native environment. By analyzing motions individual Swi6 molecules, identify mobility...
Heterochromatin is characterized by histone hypoacetylation and an enrichment of methylation marks on histones which are associated with epigenetic silencing. In fission yeast, a single H3 methyltransferase Clr4 (Suv39h) directs all the H3K9 i.e., H3K9me2/3 enforces establishment transcriptionally silent regions in genome referred to as heterochromatin. A putative demethylase, Epe1, recruited sites heterochromatin via interaction chromo‐domain containing protein Swi6, homolog HP1. The loss...
Abstract HP1 proteins bind with low affinity but high specificity to histone H3 lysine 9 methylation (H3K9me), forming transcriptionally inactive genomic compartments referred as heterochromatin. How traverse a complex and crowded chromatin landscape on the millisecond timescale H3K9me remains paradoxical. Here, we apply single-molecule imaging visualize an homolog, fission yeast Swi6, in its native environment. By analyzing Swi6 motions, identify individual mobility states that map discrete...