A5086947884- Angiogenesis and VEGF in Cancer
- Microfluidic and Capillary Electrophoresis Applications
- Monoclonal and Polyclonal Antibodies Research
- Polymer Surface Interaction Studies
- Mass Spectrometry Techniques and Applications
- Advanced Polymer Synthesis and Characterization
- Protein purification and stability
- Microtubule and mitosis dynamics
- Microfluidic and Bio-sensing Technologies
- Blood properties and coagulation
- Cell Adhesion Molecules Research
- Analytical Chemistry and Chromatography
- Cancer-related Molecular Pathways
- Hydrogels: synthesis, properties, applications
- Electrospun Nanofibers in Biomedical Applications
- Lymphatic System and Diseases
- Tissue Engineering and Regenerative Medicine
- Atherosclerosis and Cardiovascular Diseases
- Kruppel-like factors research
- Spaceflight effects on biology
- biodegradable polymer synthesis and properties
- Aortic aneurysm repair treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Coronary Interventions and Diagnostics
- Hippo pathway signaling and YAP/TAZ
Stanford University
2012-2015
β-Catenin is a multifunctional protein with critical roles in cell-cell adhesion, Wnt signaling, and the centrosome cycle. Whereas regulation of β-catenin adhesion signaling are well understood, how regulated at not. NIMA-related kinase 2 (Nek2), which regulates disjunction/splitting, binds to phosphorylates β-catenin. Using vitro cell-based assays, we show that Nek2 same regulatory sites N-terminus as glycogen synthase 3β (GSK3β), recognized by specific phospho-S33/S37/T41 antibody,...
Isotactic triads are introduced into poly(dimethylaminoethyl methacrylate) (PDMAEMA) when a Lewis acid yttrium(III)trifluoromethanesulfonate, Y(OTf)3, is present during the ATRP polymerization. The changes in tacticities of polymers modest. However, tacticity affects phase separation process but different way two studied cases, at pH 8 and 9. pH, thus charge polymer, balance between electrostatic stereochemical effects. Upon chain collapse, zeta potential polymer decreases discontinuously 9,...
The role of nanotopographical extracellular matrix (ECM) cues in vascular endothelial cell (EC) organization and function is not well-understood, despite the composition nano- to microscale fibrillar ECMs within blood vessels. Instead, predominant modulator EC traditionally thought be hemodynamic shear stress, which uniform stress induces parallel-alignment ECs with anti-inflammatory function, whereas disturbed flow a disorganized configuration pro-inflammatory function. Since acts on by...
Protein therapeutics are usually produced in heterogeneous forms during bioproduction and bioprocessing. Heterogeneity results from post-translational modifications that can yield charge variants require characterization throughout product development manufacturing. Isoelectric focusing (IEF) with UV detection is one of the most common methods to evaluate protein heterogeneity biopharmaceutical industry. To identify variant peaks, a new imaged microfluidic chip-based isoelectric (icIEF)...
Rapid, direct identification and quantitation of protein charge variants, assessment critical quality attributes with high sensitivity are important drivers required to accelerate the development biotherapeutics. We describe use an enhanced microfluidic chip-based integrated imaged capillary isoelectric focusing-mass spectrometry (icIEF-MS) technology assess multiple intact antibodies in a single run. Results demonstrate comprehensive detection variants aglycosylated knob-into-hole...
Aqueous solution and water-air interfacial properties of associative thermally responsive A-B-A stereoblock poly(N-isopropylacryl amide), PNIPAM, polymers were studied compared to atactic PNIPAM. The consist PNIPAM as a hydrophilic block (either A or B) water-insoluble isotactic surface tensions aqueous solutions measured function both temperature concentration. blocks did not have an effect on the activity solutions. Rheological measurements interface showed that aggregated PNIPAMs...
With 25 million Americans suffering from at least one clinical manifestation of atherosclerosis, there is a pressing need to better understand the biological basis for development atherosclerosis. Endothelial cells (EC) that form inner lining blood vessels are primarily responsible resisting atherosclerotic lesion formation. Although hemodynamic shear stress has been thought be dominant modulator EC function, spatial patterning cues nanoscale extracellular matrices (ECMs) underlie...