A5063313769- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Microbial metabolism and enzyme function
- Metabolism and Genetic Disorders
- Analytical Chemistry and Chromatography
- Biochemical Acid Research Studies
- Porphyrin Metabolism and Disorders
- Nuclear Physics and Applications
- Biochemical and Molecular Research
- Metalloenzymes and iron-sulfur proteins
- Microbial Metabolic Engineering and Bioproduction
- Chemical Reactions and Isotopes
- Protein Hydrolysis and Bioactive Peptides
- Protein Structure and Dynamics
- X-ray Spectroscopy and Fluorescence Analysis
- Synthesis and Reactions of Organic Compounds
- Crystallography and molecular interactions
- Crystallization and Solubility Studies
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Asymmetric Synthesis and Catalysis
- Meat and Animal Product Quality
- Hepatitis B Virus Studies
- Mass Spectrometry Techniques and Applications
- Various Chemistry Research Topics
- Biopolymer Synthesis and Applications
Hiroshima Institute of Technology
2021-2025
Osaka Research Institute of Industrial Science and Technology
2012-2021
Osaka University
1999-2021
Bioengineering Center
2016
National Center for Genetic Engineering and Biotechnology
2016
John Wiley & Sons (United States)
2016
Hudson Institute
2016
University of Washington
2009
Osaka City University
1998-2008
SPring-8
2003-2008
The mechanism of Preferential Enrichment, an unusual enantiomeric resolution phenomenon observed upon recrystallization a series racemic crystals which are classified as mixed crystal with fairly ordered arrangement the two enantiomers, has been studied. On basis existence polymorphs and occurrence resulting polymorphic transition during crystallization from solution, accounted for in terms (1) preferential homochiral molecular association to form one-dimensional chain structures...
The three-dimensional structures of pyridoxal 5'-phosphate-type aspartate aminotransferase (AspAT) from Thermus thermophilus HB8 and pyridoxamine 5'-phosphate type one in complex with maleate have been determined by X-ray crystallography at 1.8 2.6 A resolution, respectively. enzyme is a homodimer, the polypeptide chain subunit folded into arm, small domain, large domain. AspATs many species were classified subgroups Ia Ib. belongs to subgroup Ib, its sequence being less than 16% identical...
Mitochondrial mRNA editing in trypanosomatid parasites involves several multiprotein assemblies, including three very similar complexes that contain the key enzymatic activities and sediment at ~20S on glycerol gradients. These editosomes have a common set of 12 proteins, enzymes for uridylyl (U) removal addition, 2 RNA ligases, proteins with RNase III-like domains, 6 predicted oligonucleotide binding (OB) folds. In each 3 distinct contains different III-type endonuclease, 1 related and, one...
Cyclization is a promising strategy to enhance protein stability, but its applicability often limited by structural constraints such as the distance between terminal regions. Here, we report rational design and characterization of cyclized Tobacco Etch Virus protease (cTEVp) using SpyRing system, which enables covalent cyclization through SpyTag/SpyCatcher-mediated isopeptide bond formation. We applied this approach widely used engineered TEVp variant (L56V, S135G, S219V, Δ238-242) employed...
Journal Article Three-Dimensional Structure of 4-Amino-4-Deoxychorismate Lyase from Escherichia coli Get access Tadashi Nakai, Nakai *Department Chemistry, Graduate School Science, Osaka City UniversitySumiyoshi-ku, 558-8585 Search for other works by this author on: Oxford Academic PubMed Google Scholar Hisashi Mizutani, Mizutani Ikuko Miyahara, Miyahara Ken Hirotsu, Hirotsu 3 3To whom correspondence should be addressed. Phone: +81-6-6605-2557, Fax +81-6-6605-3131, E-mail-...
Histidinol-phosphate aminotransferase (HspAT) is a key enzyme on the histidine biosynthetic pathway. HspAT catalyzes transfer of amino group l-histidinol phosphate (Hsp) to 2-oxoglutarate form imidazole acetol (IAP) and glutamate. Thus, recognizes two kinds substrates, Hsp glutamate (double substrate recognition). The crystal structures native its complexes with N-(5'-phosphopyridoxyl)-l-glutamate have been solved refined R-factors 19.7, 19.1, 17.8% at 2.0, 2.2, 2.3 Å resolution,...
The sterically bulky t-Bu-substituted derivatives of 1,1,2,2-tetraphenyl-3,8-dichlorobuta[b]naphthalene (5), trans-1,2-tert-butyl-1,2-diphenyl- (8) and 1,1-di-tert-butyl-2,2-diphenyl-3,8-dichlorocyclobuta[b]naphthalene (12), were prepared. X-ray analysis 8 12 at 150 K showed that C−C bonds are 1.686 1.729 Å, respectively. latter bond length is longer than 1.720 Å in 5 the longest one reliably determined to date.
白金,イリジウム,パラジウムの放射化学的精製法を検討し,重量分析的な方法,イオン交換樹脂法,溶媒抽出法を組み合わせることにより十分満足のいく精製法が得られた。この精製法を用いて放射化分析法を確立した。試料と,試料に組成のよく似た“ mock ”に既知量の白金,イリジウム,パラジウムを加えた標準試料とをそれぞれ石英管に封じ,ならべてカプセルに入れたものを JRR-1 中で 3 日間中性子照射を行なった。照射した試料および標準試料から担体の存在の下で上述の精製法にしたがって純粋な白金,イリジウム,パラジウムをとり出し GM 計数管で測定した放射能の強さを比較して,これら 元素を定量した。分析した試料はイン鉄 種,石質イン石 種,パラサイト 1 種(金属相およびケイ酸塩相)である。
Aspartate aminotransferase (AspAT) is a unique enzyme that can react with two types of substrate quite different properties, acidic substrates, such as aspartate and glutamate, neutral although the catalytic group Lys-258 acts on both substrate. The dynamic properties substrate-binding site are indispensable to interaction hydrophobic substrates (Kawaguchi, S., Nobe, Y., Yasuoka, J., Wakamiya, T., Kusumoto, Kuramitsu, S. (1997) J. Biochem. (Tokyo) 122, 55-63). AspATs from various organisms...
The structural genes encoding quinohemoprotein amine dehydrogenase (QHNDH) in Gram-negative bacteria constitute a polycistronic operon together with several nearby genes, which are collectively termed "qhp". We previously showed that the qhpD gene, lies between qhpA and qhpC (encoding α γ subunits of QHNDH, respectively), qhpE follows qhpB β subunit), both encode enzymes specifically involved posttranslational modification subunit hence essential for QHNDH biogenesis Paracoccus denitrificans...
Quinohemoprotein amine dehydrogenase (QHNDH), an αβγ heterotrimer present in the periplasm of several Gram-negative bacteria, catalyzes oxidative deamination various aliphatic amines such as n-butylamine for assimilation carbon and energy sources. The γ subunit mature QHNDH contains a protein-derived quinone cofactor, cysteine tryptophylquinone, three intrapeptidyl thioether cross-links between Cys Asp or Glu residues. In its cytoplasmic nascent form, has 28-residue N-terminal leader peptide...
Aspartate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus HB8 (ttAspAT), has been believed to be specific for acidic substrate. However, stepwise introduction of mutations in the active-site residues finally changed its substrate specificity that a dual-substrate enzyme. The final mutant, [S15D, T17V, K109S, S292R] ttAspAT, is active toward both and hydrophobic substrates. During course mutation, activities substrates independently. mobile Arg292* residue into...
Journal Article Structural bases for the specific interactions between E2 and E3 components of Thermus thermophilus 2-oxo acid dehydrogenase complexes Get access Tadashi Nakai, Nakai * 1RIKEN SPring-8 Center, Harima Institute, Sayo, Hyogo 679-5148, Japan; 2Department Biology, Graduate School Science, Osaka University, Toyonaka, 560-0043, 3Department Chemistry, City Sumiyoshi, 558-8585, Japan *To whom correspondence should be addressed. Tel: +1-206-616-4510, Fax: +1-206-685-7002, E-mail:...
従来,微量のタングステンは,タングステンを化学的に濃縮してから,いろいろの方法で定量されてきたが,この濃縮操作にともなう誤差,試薬からの汚染などいろいろの問題点を含む。中性子放射化分析法はタングステンに対する検出感度が従来の分析法のそれより非常に高く,反応の特殊性がいちじるしい上に,試料の照射後は試薬による汚染がない。また担体の使用により化学的収率を求めることができるなどのすぐれた点を有している。著者らは186W (n.γ) 187Wにより生ずる187W (T1/2 24h,β-0.62, 1.33 MeV)を用いて,イン石およびケイ酸塩岩石中のタングステンを定量した。定量結果はつぎのとおりである(ppm)。イン鉄: Henbury0.78 Canyon Diable 1.20, Admire(金属相)0.028。イン石:ForestCity 0.24, Modoc 0.13, Nuevo 0Laredao O.11。岩石:花コウ岩 0.11～0.66, 安山岩 1.06, 玄武岩 0.21,超塩基性岩 0.19,堆積岩1.29,
The title compound was found to form an inclusion complex crystal with various kinds of guests. It also that crystalline lattice some is chiral. By enantioselective complexation in the chiral lattice, racemic guest resolved. studied by X-ray analysis. Solid-state synthesis host described. Copyright © 2000 John Wiley & Sons, Ltd.