A5049538788- Retinoids in leukemia and cellular processes
- Estrogen and related hormone effects
- Antioxidant Activity and Oxidative Stress
- Nuclear Receptors and Signaling
- Animal Nutrition and Physiology
- Tissue Engineering and Regenerative Medicine
- Effects of Environmental Stressors on Livestock
- RNA Interference and Gene Delivery
- Microfluidic and Capillary Electrophoresis Applications
- HER2/EGFR in Cancer Research
- 3D Printing in Biomedical Research
- Mass Spectrometry Techniques and Applications
- Cutaneous lymphoproliferative disorders research
- Biotin and Related Studies
- Reproductive System and Pregnancy
- Molecular Biology Techniques and Applications
- Rabbits: Nutrition, Reproduction, Health
- Photoreceptor and optogenetics research
- Click Chemistry and Applications
- Photochromic and Fluorescence Chemistry
- Synthesis and Properties of Aromatic Compounds
- Planarian Biology and Electrostimulation
- Biocrusts and Microbial Ecology
- Workplace Health and Well-being
- Structural and Chemical Analysis of Organic and Inorganic Compounds
National Hospital Organization
2025
Kyushu Hospital
2025
Okayama University
2008-2023
Nagoya University
1997-2022
Kagoshima University
2022
Tokyo University of Agriculture
2021
Hitachi (Japan)
2004-2010
Hitachi High-Tech (Japan)
2010
RIKEN Center for Brain Science
2009
Kyushu University
2006
Mouse mothers transfer metabolic mode Obesity and diseases tend to go together, humans who become obese are also prone type 2 diabetes cardiovascular problems. Starting with the observation that offspring of germ-free mice tended on high-fat diets, Kimura et al. investigated how presence microbiota might be protective in (see Perspective by Ferguson). Short-chain fatty acids (SCFAs) from known suppress insulin signaling reduce fat deposition adipocytes. Further experiments showed SCFAs...
Reports of autoimmune diseases coexisting with pulmonary alveolar proteinosis (autoimmune PAP; APAP) are extremely rare. APAP may often be misdiagnosed as connective tissue disease–associated interstitial lung disease (ILD). Here, we describe a rare case patient systemic sclerosis who was diagnosed after the exacerbation opacities during treatment immunosuppressive agents. A 72-year-old woman (SSc) at age 68, and initiated prednisolone (PSL). At 70, she ILD associated SSc. Despite...
We developed a new passive-type micromixer based on the baker's transformation and realized fast mixing of protein solution, which has lower diffusion constant. The is an ideal method, but there no report microfluidic (MBT), since it required to fabricate complicated three-dimensional (3D) structure realize MBT device. In this note, we successfully device by using precision diamond cutting oxygen-free copper substrate for mould fabrication PDMS replication. with 10.4 mm length enables us...
Phosphatidylglucoside (PtdGlc) is a recently discovered unique glycophospholipid involved in granulocytic differentiation of human promyelocytic leukemia cell line HL60 and astrocytic developing rodent brains. Using PtdGlc-specific monoclonal antibody immunofluorescence immunoelectron microscopy, we showed that PtdGlc forms distinct lipid domains on the outer leaflet plasma membrane cells alveolar epithelial line, A549. Similar to glycosphingolipid, glucosylceramide (GlcCer), natural form...
Adipose tissue-derived stem cells (ASCs) have shown promise in cell therapy because of their ability to self-renew damaged or diseased organs and easy harvest. To ensure the distribution quantification ASCs injected from tail vein, several whole-body imaging techniques including fluorescence optical with quantum dots (QDs) been employed, but they may suffer insufficient sensitivity accuracy. Here, we report quantitative various (heart, lung, liver, spleen, kidney) mice, which were...
The sterically bulky t-Bu-substituted derivatives of 1,1,2,2-tetraphenyl-3,8-dichlorobuta[b]naphthalene (5), trans-1,2-tert-butyl-1,2-diphenyl- (8) and 1,1-di-tert-butyl-2,2-diphenyl-3,8-dichlorocyclobuta[b]naphthalene (12), were prepared. X-ray analysis 8 12 at 150 K showed that C−C bonds are 1.686 1.729 Å, respectively. latter bond length is longer than 1.720 Å in 5 the longest one reliably determined to date.
A number of factors including low stoichiometry phosphorylation, ion suppression, and reduced peptide backbone fragmentation interfere with precise identification proteins in phosphoproteomic analysis by MS. Therefore, enrichment phosphopeptides is an important process for subsequent mass spectrometric analysis. Here, we have developed a simple efficient method enrichment, which employs biphasic phosphate-binding tag (Phos-tag)/C18 tip consisting overlaid Phos-tag on the C18 resin pipet tip....
Stem cell transplantation has been expected to have various applications for regenerative medicine. However, in order detect and trace the transplanted stem cells body, non-invasive widely clinically available imaging technologies are required. In this paper, we focused on magnetic resonance (MR) technology, investigated whether trimethylamino dextran-coated iron oxide nanoparticle -03 (TMADM-03), which was newly developed by our group, could be used labeling adipose tissue-derived (ASCs) as...
Inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease, is an intractable of the gastrointestinal tract. Multiple environmental factors, food ingredients, have been implicated in development these diseases. For example, animal fat-rich diets are predisposing factors for colitis, whereas n-3 unsaturated fatty acids such as docosahexaenoic acid (DHA) show protective effects experimental negatively correlated with incidence disease. Given that DHA exhibits agonistic...
Quantum dots (QDs) are thought to be a novel inorganic probe for in vivo fluorescence imaging because of their excellent properties. Autofluorescence is generally known produced from various living bodies including humans, rats, and mice. However, the influence autofluorescence on using QDs remains poorly understood. In this article, we assessed derived mouse body QDs. The dorsal ventral which hair was removed were detected under all kinds excitation/fluorescence filter settings (blue,...
The retinoid X receptor (RXR) partial agonist 1-[(3,5,5,8,8-pentamethyl-5,6,7,8-tetrahydronaphthalen-2-yl)amino]benzotriazole-5-carboxylic acid (1; CBt-PMN, Emax = 75%, EC50 143 nM) is a candidate for treatment of central nervous system (CNS) diseases such as Alzheimer's and Parkinson's based on reports that RXR-full 4-[1-(3,5,5,8,8-pentamethyl-5,6,7,8-tetrahydro-2-naphthyl)ethynyl]benzoic (bexarotene) shows therapeutic effects these disease in rodent models. Here, we synthesized...
Retinoid X receptor (RXR) heterodimers such as PPAR/RXR, LXR/RXR, and FXR/RXR can be activated by RXR agonists alone are therefore designated permissive. Similarly, existing antagonists show allosteric antagonism toward partner in these permissive heterodimers. Here, we 1-(3-(2-ethoxyethoxy)-5,5,8,8-tetramethyl-5,6,7,8-tetrahydronaphthalen-2-yl)-2-(trifluoromethyl)-1H-benzo[d]imidazole-5-carboxylic acid (14, CBTF-EE) the first antagonist that does not inhibition This compound was designed...
Retinoid X receptor (RXR) is a nuclear that heterodimerizes with several receptors, integrating ligand-mediated signals across the heterodimers. Synthetic RXR agonists have been developed to cure certain inflammatory diseases, including bowel diseases (IBDs). However, pre-existing agonists, which are lipophilic and readily absorbed in upper intestine, cause considerable adverse effects such as hepatomegaly, hyperlipidemia, hypothyroidism. To minimize these effects, we an agonist, NEt-3IB,...
Bexarotene, a retinoid X receptor (RXR) agonist, is used to treat cutaneous T-cell lymphoma, and drug repositioning research has also been reported, despite warnings of teratogenicity. However, fetal transfer bexarotene its effect on rat bone formation have not examined. In this study, we conducted detailed teratogenicity transferability assessment in rats. Repeated administration during pregnancy caused marked atrophy dysplasia. Although was detectable by dynamic imaging [11C]bexarotene...
Ligands for retinoid X receptors (RXRs), "rexinoids", are attracting interest as candidates therapy of type 2 diabetes and Alzheimer's Parkinson's diseases. However, current screening methods rexinoids slow require special apparatus or facilities. Here, we created 7-hydroxy-2-oxo-6-(3,5,5,8,8-pentamethyl-5,6,7,8-tetrahydronaphthalen-2-yl)-2H-chromene-3-carboxylic acid (10, CU-6PMN) a new fluorescent RXR agonist developed system using 10. Compound 10 was designed based on the fact that...
Ribosomal protein S14 can be classified into three types. The first, the C+ type has a Zn2+ binding motif and is ancestral. second third are C- short long types, neither of which contain ca. 90 residues 100 in length, respectively. In present study, from Bacillus subtilis ribosomes (S14BsC+) were completely replaced by heterologous Escherichia coli (S14Ec) or Synechococcus elongatus (S14Se). Surprisingly, S14Ec S14Se incorporated fully 70S B. However, growth rates as well sporulation...
Retinoid X receptor (RXR) modulators (rexinoids) are considered to have therapeutic potential for multiple diseases, such as Alzheimer's disease and Parkinson's disease. To overcome various disadvantages of prior screening methods, we previously developed an RXR binding assay using a fluorescent ligand, CU-6PMN (4). However, this ligand binds not only at the ligand-binding domain (LBD) but also dimer–dimer interface hRXRα. Here, present new antagonist...
The in vitro metabolism of phenelzine (2-phenylethylhydrazine) by phenobarbital-pretreated rat liver microsomes yields ethylbenzene, 2-phenylethanol, 2-phenylacetaldehyde, benzaldehyde, benzylalcohol, and toluene as metabolites. Isotopic studies demonstrate that the oxygen atom 2-phenylethanol derives from molecular this alcohol is not produced reduction 2-phenylacetaldehyde. rates destruction cytochrome P-450, accumulation spin-trapped 2-phenylethyl radicals, formation ethylbenzene are same...
Retinoid X receptor (RXR) ligands often bind in modes which the carboxy group forms a hydrogen bond inside ligand-binding pocket (LBP). However, our previously reported RXR antagonist, CBTF-EE (4a), binds with its directed outside LBP and alkoxy side chain located LBP. Here, we examined binding of 4b 4c bearing nitrobenzoxadiazole (NBD) or boron-dipyrromethene (BODIPY) fluorophore, respectively, at end 4a. Both compounds function as antagonists. 4c, but not 4b, was available for fluorescence...