A5042911993- Immune Cell Function and Interaction
- Helicobacter pylori-related gastroenterology studies
- Gut microbiota and health
- Probiotics and Fermented Foods
- T-cell and B-cell Immunology
- SARS-CoV-2 and COVID-19 Research
- Retinoids in leukemia and cellular processes
- Immunotherapy and Immune Responses
- Viral gastroenteritis research and epidemiology
- Immunodeficiency and Autoimmune Disorders
- Immune responses and vaccinations
- Regulation of Appetite and Obesity
- Chronic Lymphocytic Leukemia Research
- Dietary Effects on Health
- Galectins and Cancer Biology
- Microscopic Colitis
- Gastrointestinal motility and disorders
- Reproductive System and Pregnancy
- Inflammatory Bowel Disease
- Asthma and respiratory diseases
- Glycosylation and Glycoproteins Research
- Eosinophilic Esophagitis
- CAR-T cell therapy research
- Cytokine Signaling Pathways and Interactions
- Vitamin D Research Studies
Okinawa Institute of Science and Technology Graduate University
2022-2025
Keio University
2018-2024
BackgroundThe dysbiosis of gut microbiota has been implicated in the pathogenesis inflammatory bowel diseases; however, underlying mechanisms have not yet elucidated. Heavily glycosylated mucin establishes a first-line barrier against pathogens and serves as niche for microbial growth.MethodsTo elucidate relationships among dysbiosis, abnormal utilisation, metabolic dysfunction, we analysed short-chain fatty acids (SCFAs) components stool samples 40 healthy subjects, 49 ulcerative colitis...
Aerobic glycolysis, a metabolic pathway essential for effector T cell survival and proliferation, regulates differentiation of autoimmune helper (Th) 17 cells, but the mechanism underlying this regulation is largely unknown. Here, we identify glycolytic intermediate metabolite, phosphoenolpyruvate (PEP), as negative regulator Th17 differentiation. PEP supplementation or inhibition downstream enzymes in differentiating cells increases intracellular levels inhibits interleukin (IL)-17A...
Abstract COVID-19 mRNA vaccines induce protective adaptive immunity against SARS-CoV-2 in most individuals, but there is wide variation levels of vaccine-induced antibody and T-cell responses. However, the mechanisms underlying this inter-individual remain unclear. Here, using a systems biology approach based on multi-omics analyses human blood stool samples, we identified several factors that are associated with immune BNT162b2-induced T cell response positively late monocyte responses...
The AP-1 transcription factor JunB is essential for the differentiation of pathogenic T helper 17 (Th17) cells, which are key mediators autoimmune diseases such as multiple sclerosis and colitis. While importance during Th17 polarization known, its role in mature cells-critical therapeutic targets these diseases-remains unclear. In this study, we employed dTAG system, a targeted protein degradation approach, to deplete cells generated both vitro vivo. During cell differentiation, replicated...
Immunomodulatory function of all-trans retinoic acid (ATRA) has been gathering much attention for the therapy autoimmune diseases. ATRA is a chemically unstable molecule which requires proper formulation targeted delivery. Here we examined nanostructured lipid carrier (NLC) ATRA. NLC representative nanoparticle especially suited oral We established preparation procedures ATRA-containing (NLC-RA) minimizes degradation during process. NLC-RA thus obtained was taken up by macrophages and...
Inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease, is an intractable of the gastrointestinal tract. Multiple environmental factors, food ingredients, have been implicated in development these diseases. For example, animal fat-rich diets are predisposing factors for colitis, whereas n-3 unsaturated fatty acids such as docosahexaenoic acid (DHA) show protective effects experimental negatively correlated with incidence disease. Given that DHA exhibits agonistic...
Inflammatory bowel disease (IBD) is a refractory of the gastrointestinal tract that believed to develop in genetically susceptible individuals. Glycosylation, type post-translational modification, involved development wide range diseases, including IBD, by modulating function various glycoproteins. To identify novel genes contributing we analyzed single nucleotide polymorphisms (SNPs) glycosylation-related IBD patients and identified MAN2A1, encoding alpha-mannosidase II (α-MII), as...
The effectiveness of vaccines in preventing infection from specific pathogens is closely related to the magnitude antigen-specific T cell and antibody responses induced by vaccine. These depend on immune states individual, which shaped genetic environmental factors. Recent studies using omics technologies identified cells, genes, gut microbial species metabolic pathways at baseline or during early vaccine as correlates responses. findings shed light molecular cellular mechanisms underlying...
Intestinal epithelial cells (IECs) serve as robust barriers against potentially hostile luminal antigens and commensal microbiota. Epithelial barrier dysfunction enhances intestinal permeability, leading to leaky gut syndrome (LGS) associated with autoimmune chronic inflammatory disorders. However, a causal relationship between LGS systemic disorders remains unclear. Ap1m2 encodes clathrin adaptor protein complex 1 subunit mu 2, which facilitates polarized trafficking toward the basolateral...
Basic-leucine zipper transcription factor ATF-like (BATF) and interferon regulatory 4 (IRF4) are crucial factors for generation of cytotoxic effector memory CD8+ T cells. JunB is required expression genes controlled by BATF IRF4 in CD4+ cell responses, but the role cells remains unknown. Here, we demonstrate that essential responses. transiently induced, depending on receptor (TCR) signal strength. deficiency severely impairs clonal expansion response to acute infection with Listeria...
Abstract COVID-19 mRNA vaccines induce protective adaptive immunity against SARS-CoV-2 in most individuals, but there is wide variation levels of vaccine-induced antibody and T-cell responses. However, factors associated with this inter-individual remain unclear. Here, using a systems biology approach based on multi-omics analyses human blood stool samples, we find that baseline expression AP-1 transcription factors, FOS ATF3 , inversely correlated BNT162b2 modules related to immunity, it...
Abstract The dysbiosis of gut microbiota has been implicated in the pathogenesis inflammatory bowel diseases (IBDs); however, underlying mechanisms have not yet elucidated. Heavily glycosylated mucin only establishes a first-line barrier against pathogens, but also serves as niche for microbial growth. We hypothesized that may cause abnormal utilization and metabolic dysfunction. To test this hypothesis, we analyzed short-chain fatty acids (SCFAs) components stool samples 40 healthy...
Background: The dysbiosis of gut microbiota has been implicated in the pathogenesis inflammatory bowel diseases (IBDs); however, underlying mechanisms have not yet elucidated. Heavily glycosylated mucin only establishes a first-line barrier against pathogens, but also serves as niche for microbial growth.Methods: To elucidate relationships between dysbiosis, abnormal utilization, and metabolic dysfunction, we analyzed short-chain fatty acids (SCFAs) components stool samples 40 healthy...