Major barriers to delivery of biomolecules are crossing the cellular membranes and achieving a high cytoplasmic concentration by circumventing entrapment into endosomes other lytic organelles. Motivated such aim, we have investigated capability multiwalled carbon nanotubes (MWCNTs) penetrate cell membrane plant protoplasts (plant cells made devoid their walls via enzymatic treatment) studied internalization mechanism confocal imaging TEM techniques. Our results indentified an...

Using cysteine and its derivatives as capping molecules, we investigated the influence of physical structure chemical nature molecules on selective growth stabilization small CdSe nanoparticles (NPs) in aqueous solution at room temperature. Our investigations revealed specific roles for each functional group cysteine, could correlate this with size, size restriction, distribution, stability NPs. For NPs solution, their should have least one strong nucleophilicity well another free, charged...

We present a new rapid and easy-to-use immunoassay chip which we have named the immuno-pillar chip. It has hydrogel pillars, fabricated inside microchannel, with many antibody molecules immobilized onto 1 µm diameter polystyrene beads. To evaluate performance, applied it to sandwich assay of C-reactive protein (CRP), α-fetoprotein (AFP) prostate-specific antigen (PSA), cardiac inflammation marker, tumors prostate cancer markers, respectively. For detection disease confirmed provides analysis...

We report aqueous phase synthesized semiconductor nanoparticles with well-defined numbers of constituent atoms. Aqueous synthesis provides many advantages over organic for producing such high-quality nanoparticles. CdSe excellent colloidal and optical stabilities directly in solution at room temperature then identified them as selectively grown (CdSe)33 (CdSe)34 magic-sized clusters. These clusters displayed extremely sharp excitonic absorption emission peaks because their practically...

As nanoparticles can cross different cellular barriers and access tissues, control of their uptake fate presents a functional approach that will be broadly applicable to nanoscale technologies in cell biology. Here we show the trafficking single-walled carbon nanotubes (SWCNTs) through various subcellular membranes plant is facilitated or inhibited by attaching suitable tag controlling medium components. This enables unique over distribution SWCNTs provides key strategy promote elimination...

Oleic acid (OA) and oleates form self-assembled structures dispersible in aqueous media. Herein, the physicochemical properties of OA/oleate assemblies were characterized using fluorescent probes Raman spectroscopy, under relatively high dilution (<100 mM total amphiphile) at 25 °C. Anisotropy analysis 1,6-diphenyl-1,3,5-hexatriene showed that microviscosity assembly was highest pH 7.5 (the range 6.9-10.6 investigated). The fluorescence spectra 6-lauroyl-2-dimethylaminonaphthalene revealed...

Summary The Arabidopsis ACCELERATED CELL DEATH 2 (ACD2) protein protects cells from programmed cell death (PCD) caused by endogenous porphyrin‐related molecules like red chlorophyll catabolite or exogenous protoporphyrin IX. We previously found that during bacterial infection, ACD2, a breakdown enzyme, localizes to both chloroplasts and mitochondria in leaves. Additionally, acd2 show mitochondrial dysfunction. In plants with ACD2 + sectors, functions autonomously, implicating pro‐death...

Here we report that nanopillar array structures have an intrinsic ability to suppress electroosmotic flow (EOF). Currently using glass chips for electrophoresis requires laborious surface coating control EOF, which works as a counterflow the mobility of negatively charged samples such DNA and sodium dodecyl sulfate (SDS) denatured proteins. Due carried out SDS-protein complexes in without adding any reagent protein adsorption EOF. We also show EOF profile inside region was deformed inverse...

The dynamic properties of phospholipid (PL) membranes (phase state and phase transition) play crucial roles in biological systems. However, highly sensitive, direct analytical methods that shed light on the nature lipids their assemblies have not been developed to date. Here, we describe analysis PL-modified Au nanoparticles (Au@PL) using membrane surface-enhanced Raman spectroscopy (MSERS) report self-assembled PL nanoparticle. intensity per concentration increased by 50–170 times with...

The phase behavior, Na+ coordination structures, and transport electrochemical properties of binary mixtures NaN(SO2F)2 (NaFSA) sulfolane (SL) solvent were investigated. NaFSA SL form stable solvates at molar ratios [NaFSA]/[SL] = 1/3 1/0.5, there is a crystallinity gap in the range 1/1.6 ≤ 1/0.8. In crystals both NaFSA–(SL)3 NaFSA–(SL)0.5, solvent-bridged Na+–SL–Na+ anion-bridged Na+–FSA–Na+ structures are formed. gap, remain liquid room temperature can be regarded as molten NaFSA. Raman...

Numerous research studies have been done for exosomes, particularly focusing on membrane proteins and included nucleic acids, the volume of knowledge about lipids in exosomal has increasing. However, dynamic property is hardly studied. By employing milk exosome as an example, herein was characterized fluidity polarity. The lipid composition phase state (exosome from bovine milk) were estimated. contained enriched Chol (43.6 mol % total extracts), which made liquid-ordered (lo) by interacting...

Chitosan-based cryogel particles were synthesized using the inverse Leidenfrost (iLF) effect, with glutaraldehyde employed as cross-linker. The resulting cryogels exhibited a sponge-like morphology micrometer-sized interconnected pores and demonstrated resilience, withstanding up to three compression-release cycles. These characteristics highlight potential of chitosan for diverse applications, including adsorption biomedical uses. We further investigated influence varying acetic acid...

This research aims to deepen the understanding of relationship between conductivity and morphology in polypyrrole (PPy) via a comparison bipolaron polaron ratios with focus on C-H deformation area. PPy samples were synthesized different surfactants: sodium dodecyl sulfate (SDS), cetyltrimethylammonium bromide (CTAB), tween 80 (TW). study revealed that SDS significantly altered region showed higher than other surfactants. Notably, morphological shifts sheet-like structure when using ammonium...

This study aims to develop a biomimetic nano-drug delivery system (nano-DDS) by employing macrophage cell membrane camouflaging strategy modify lyotropic liquid crystal nanoparticles (LLC-NPs).

Previous reports have shown that the liver-type glucose transporter GLUT2 gene expression is upregulated in liver of obese and diabetic animals human subjects. This phenomenon correlated with an increased output from liver; however, regulatory mechanism not clear. To investigate relationship between hepatic steatosis, frequently found patients, expression, we developed oleic acid-induced vitro fatty model. Lipid-accumulated cells morphologically mimicking steatosis were successfully induced...

Adipose tissue-derived stem cells (ASCs) have a self-renewing ability and can be induced to differentiate into various types of mesenchymal tissue. Because their potential for clinical application, it has become desirable label the tracing transplanted in vivo imaging. Quantum dots (QDs) are novel inorganic probes that consist CdSe/ZnS-core/shell semiconductor nanocrystals recently been explored as fluorescent cell labeling. In this study, negatively charged QDs655 were applied ASCs...

We developed a new passive-type micromixer based on the baker's transformation and realized fast mixing of protein solution, which has lower diffusion constant. The is an ideal method, but there no report microfluidic (MBT), since it required to fabricate complicated three-dimensional (3D) structure realize MBT device. In this note, we successfully device by using precision diamond cutting oxygen-free copper substrate for mould fabrication PDMS replication. with 10.4 mm length enables us...

Fatty acids (FAs) are known to form vesicle structures, depending on the surrounding pH conditions. In this study, we prepared vesicles by mixing FAs and a cationic surfactant, then investigated their physicochemical properties using fluorescence spectroscopy dielectric dispersion analysis (DDA). The assemblies formed from oleic acid (OA) linoleic (LA) were modified adding didecyldimethylammonium bromide (DDAB). phase state of FA/DDAB mixtures was with titration curves turbidity...

We investigated the key factors that affect enantioselective adsorption of ibuprofen (IBU) on a liposome membrane by changing its lipid composition: shows different fluidity, surface charge, content chiral components, and heterogeneity (nanodomain). Nonspecific interactions (hydrophobic electrostatic) were revealed to be an important factor in enhancing adsorbed amount IBU, based experiments carried out using single lipids (DPPC, DMPC, DOPC, DLPC) positively charged liposomes (DOTAP...

Polypyrrole (PPy) is a conductive polymer known for its biocompatibility and ease of synthesis. Chemically polymerized PPy was synthesized in the presence sodium dodecyl sulfate (SDS), showing correlations among chemical properties, physical morphology, electrical properties. Focused synthesis parameters included pyrrole (Py) concentration, SDS ammonium persulfate (APS)/Py ratio. The addition during polymerization influenced morphology by altering self-assembling process via micelle...