A5053864880- X-ray Diffraction in Crystallography
- Innovative Microfluidic and Catalytic Techniques Innovation
- Crystallization and Solubility Studies
- Enzyme Structure and Function
- Microfluidic and Capillary Electrophoresis Applications
- Chemical Synthesis and Analysis
- Thermochemical Biomass Conversion Processes
- Microbial metabolism and enzyme function
- Amino Acid Enzymes and Metabolism
- Porphyrin Metabolism and Disorders
- Ubiquitin and proteasome pathways
- Protein Structure and Dynamics
- Cancer-related Molecular Pathways
- Advanced Proteomics Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Antimicrobial Peptides and Activities
- Endoplasmic Reticulum Stress and Disease
- Enzyme Catalysis and Immobilization
- Cardiac Imaging and Diagnostics
- Biochemical Acid Research Studies
- Coronary Interventions and Diagnostics
- DNA Repair Mechanisms
- Granular flow and fluidized beds
- Carbohydrate Chemistry and Synthesis
- Protein purification and stability
Kyushu Tokai University
2012-2025
Kyushu University
2002-2023
Kwansei Gakuin University
2008-2021
Tokyo Medical University
2021
Graduate School USA
2020
Osaka University
1994-2019
Pharmac
2019
Ono Clinic
2018
National Institute of Advanced Industrial Science and Technology
2008-2017
Tokushima Prefectural Central Hospital
2011-2017
The crystal structure of bovine heart cytochrome c oxidase at 2.8 Å resolution with an R value 19.9 percent reveals 13 subunits, each different from the other, five phosphatidyl ethanolamines, three glycerols and two cholates, hemes A, copper, one magnesium, zinc. Of 3606 amino acid residues in dimer, 3560 have been converged to a reasonable by refinement. A hydrogen-bonded system, including propionate heme (heme a), part peptide backbone, imidazole ligand Cu , could provide electron...
The high resolution three-dimensional x-ray structure of the metal sites bovine heart cytochrome c oxidase is reported. Cytochrome largest membrane protein yet crystallized and analyzed at atomic resolution. Electron density distribution oxidized 2.8 Å indicates a dinuclear copper center with an unexpected similar to [2Fe-2S]-type iron-sulfur center. Previously predicted zinc magnesium have been located, former bound by nuclear encoded subunit on matrix side membrane, latter situated between...
Crystal structures of bovine heart cytochrome c oxidase in the fully oxidized, reduced, azide-bound, and carbon monoxide–bound states were determined at 2.30, 2.35, 2.9, 2.8 angstrom resolution, respectively. An aspartate residue apart from O 2 reduction site exchanges its effective accessibility to matrix aqueous phase for one cytosolic concomitantly with a significant decrease pK carboxyl group, on metal sites. The movement indicates as proton pumping site. A tyrosine acidified by...
Multisite interactions and the formation of ternary or higher-order protein complexes are ubiquitous features interactions. Cooperativity between different ligands is a hallmark for information transfer, frequently critical biological function. We describe new computational platform global analysis isothermal titration calorimetry (ITC) data study binary multisite interactions, implemented as part public domain multimethod software SEDPHAT. The titrations performed in orientations was...
The crystal structures of the copper enzyme phenylethylamine oxidase from Gram-positive bacterium Arthrobacter globiformis (AGAO) have been determined and refined for three forms enzyme: holoenzyme in its active form (at 2.2 Å resolution), an inactive 2.8 apoenzyme resolution). has a topaquinone (TPQ) cofactor formed by post-translational modification tyrosine residue presence Cu2+. Significant differences between AGAO are limited to site. polypeptide fold is closely similar those amine...
Melanin, which is responsible for virtually all visible skin, hair, and eye pigmentation in humans, synthesized, deposited, distributed subcellular organelles termed melanosomes. A comprehensive determination of the protein composition this organelle has been obstructed by melanin present. Here, we report a novel method removing that includes in-solution digestion immobilized metal affinity chromatography (IMAC). Together with in-gel digestion, allowed us to characterize melanosome proteomes...
The thermal behavior and molecular interaction of a new type bacterial copolyester, poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), P(HB-co-HHx) (HHx = 12 mol %), was investigated by using wide-angle X-ray diffraction (WAXD) differential scanning calorimetry (DSC). WAXD measurements were carried out over temperature range from 25 to 110 °C in the scattering angle 2θ 5−13°. pattern at room shows that copolymer has an orthorhombic system (α β γ 90°) with 5.76 Å, b 13.20 c 5.96 Å (fiber repeat),...
Enzymes are biocatalysts. They useful in environmentally friendly production processes and have high potential for industrial applications. However, because of problems with operational stability, cost, catalytic efficiency, many enzymatic limited The use cross-linked enzyme aggregates (CLEAs) has been introduced as an effective carrier-free immobilization method. This method is attractive it simple robust, unpurified enzymes can be used. Coimmobilization different achieved. CLEAs generally...
The ataxia telangiectasia mutated (ATM) kinase is a key tumor suppressor that regulates numerous cell cycle checkpoints as well apoptosis. Here, we report ATM critical player in the regulation of apoptosis and lymphomagenesis presence c-myc. In turn, deletion inhibitory phosphatase, Wip1, results up-regulation suppression Eμ-myc–induced B lymphomas. Using mouse genetic crosses, show onset myc-induced lymphomas dramatically delayed Wip1-null mice an ATM- p53-, but not p38 MAPK– or Arf-,...
The definite structure and chemical stability of a new glucoside L-ascorbic acid (AA) which was enzymatically glucosylated with rat intestinal rice seed α-glucosidases were reported. this AA derivative in water under aerobic conditions proved by its remarkable resistance against enhanced oxidative degradation heat, Cu2+ ion or ascorbate oxidase, it found to have no reducing activity toward radicals. These properties obviously distinguishable from those AA. This effectively hydrolyzed...