A5103164169- Enzyme Structure and Function
- Photosynthetic Processes and Mechanisms
- Protein Structure and Dynamics
- Microfluidic and Capillary Electrophoresis Applications
- Advanced X-ray Imaging Techniques
- Microfluidic and Bio-sensing Technologies
- X-ray Spectroscopy and Fluorescence Analysis
- Aldose Reductase and Taurine
- Neonatal Health and Biochemistry
- Photoreceptor and optogenetics research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nuclear Physics and Applications
- Spectroscopy and Quantum Chemical Studies
- Folate and B Vitamins Research
- Electron and X-Ray Spectroscopy Techniques
- Mass Spectrometry Techniques and Applications
- Microbial Inactivation Methods
- Receptor Mechanisms and Signaling
- Porphyrin Metabolism and Disorders
- Nicotinic Acetylcholine Receptors Study
- Radiation Detection and Scintillator Technologies
- Electrochemical sensors and biosensors
- Amino Acid Enzymes and Metabolism
- Advanced Electron Microscopy Techniques and Applications
- Meat and Animal Product Quality
Paul Scherrer Institute
2014-2025
Centre National de la Recherche Scientifique
2004-2021
CEA Grenoble
2021
European Synchrotron Radiation Facility
1999-2021
Institut de Biologie Structurale
2009-2021
Max Planck Institute for Marine Microbiology
2021
Université Grenoble Alpes
2009-2021
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2021
Swiss Light Source
2004-2019
Vifor Pharma (Switzerland)
2014
The crystal structure of bovine heart cytochrome c oxidase at 2.8 Å resolution with an R value 19.9 percent reveals 13 subunits, each different from the other, five phosphatidyl ethanolamines, three glycerols and two cholates, hemes A, copper, one magnesium, zinc. Of 3606 amino acid residues in dimer, 3560 have been converged to a reasonable by refinement. A hydrogen-bonded system, including propionate heme (heme a), part peptide backbone, imidazole ligand Cu , could provide electron...
The high resolution three-dimensional x-ray structure of the metal sites bovine heart cytochrome c oxidase is reported. Cytochrome largest membrane protein yet crystallized and analyzed at atomic resolution. Electron density distribution oxidized 2.8 Å indicates a dinuclear copper center with an unexpected similar to [2Fe-2S]-type iron-sulfur center. Previously predicted zinc magnesium have been located, former bound by nuclear encoded subunit on matrix side membrane, latter situated between...
Crystal structures of bovine heart cytochrome c oxidase in the fully oxidized, reduced, azide-bound, and carbon monoxide–bound states were determined at 2.30, 2.35, 2.9, 2.8 angstrom resolution, respectively. An aspartate residue apart from O 2 reduction site exchanges its effective accessibility to matrix aqueous phase for one cytosolic concomitantly with a significant decrease pK carboxyl group, on metal sites. The movement indicates as proton pumping site. A tyrosine acidified by...
The PILATUS 1M detector is a hybrid pixel array with over one million pixels that operate in single photon counting mode. detector, designed for macromolecular crystallography, the largest currently use at synchrotron. It modular system consisting of 18 multichip modules covering an area 21 cm × 24 cm. design components as well manufacturing including bump-bonding was performed Paul Scherrer Institute (PSI). protein crystallography requires detailed studies charge collection properties...
All the hues, even blues Photosynthetic organisms must balance maximizing productive light absorption and protecting themselves from too much light, which causes damage. Both tasks require pigments—chlorophylls carotenoids—which absorb energy either transfer it to photosystems or disperse as heat. Wang et al. determined structure of a fucoxanthin chlorophyll a/c–binding protein (FCP) diatom. The reveals arrangement specialized photosynthetic pigments in this light-harvesting protein....
The Rhesus (Rh) proteins are a family of integral membrane found throughout the animal kingdom that also occur in number lower eukaryotes. significance Rh derives from their presence human red blood cell membrane, where they constitute second most important group antigens used transfusion medicine after ABO group. related to ammonium transport (Amt) protein and there is considerable evidence that, like Amt proteins, function as ammonia channels. We have now solved structure rare bacterial...
The third beamline for macromolecular crystallography at the superbend magnet X06DA of Swiss Light Source (SLS) was designed maximum efficiency and a high degree automation to serve needs both academic industrial users. An integral part concept is crystallization facility built adjacent experimental minihutch. This unique configuration allows users request evaluate experiments using nanodispensing robots automated imaging systems. Moreover, integration platform enables test initial hits...
We demonstrate absolute quantitative mass density mapping in three dimensions of frozen-hydrated biological matter with an isotropic resolution 180 nm. As model for a system we use Chlamydomonas cells buffer solution confined microcapillary. ptychographic X-ray computed tomography to image the entire specimen, including 18 μm-diameter capillary, thereby providing directly measurement uncertainty about 6%. The resulting maps have sufficient contrast distinguish from surrounding ice and...
Increasing the data acquisition rate of X-ray diffraction images for macromolecular crystals at room temperature synchrotrons has potential to significantly accelerate both structural analysis biomolecules and structure-based drug developments. Using lysozyme model crystals, we demonstrated rapid datasets by combining a high frame pixel array detector with ultrasonic acoustic levitation protein in liquid droplets. The spinning crystal within levitating droplet ensured an efficient sampling...
Abstract The X‐ray structures of human aldose reductase holoenzyme in complex with the inhibitors Fidarestat (SNK‐860) and Minalrestat (WAY‐509) were determined at atomic resolutions 0.92 Å 1.1 Å, respectively. hydantoin succinimide moieties interacted conserved anion‐binding site located between nicotinamide ring coenzyme active residues Tyr48, His110, Trp111. Minalrestat's hydrophobic isoquinoline was bound an adjacent pocket lined by Trp20, Phe122, Trp219, bromo‐fluorobenzyl group inside...
Abstract Acoustic levitation has attracted attention in terms of chemical and biochemical analysis combination with various analytical methods because its unique container-less environment for samples that is not reliant on specific material characteristics. However, loading very high viscosity difficult. To expand the scope, we propose use polymer thin films as sample holders, whereby dispensed a film subsequently loaded onto an acoustic levitator. When applied protein crystallography...
Crystal diffraction data of heart fatty acid binding protein (H-FABP) in complex with oleic were measured at room temperature high-resolution X-ray and neutron crystallography (0.98 1.90 Å resolution, respectively). These provided very detailed information about the cluster water molecules bound H-FABP large internal cavity. The jointly refined X-ray/neutron structure was complemented by a transferred multipolar electron-density distribution using parameters ELMAMII library. resulting...
The macromolecular crystallography (MX) beamline X06DA-PXIII at the Swiss Light Source (SLS) has undergone a significant upgrade in preparation for SLS 2.0. After 15 years of operation and >2,500 PDB depositions, this highly productive superbend magnet been completely rebuilt with new optics experimental hutches. design, which aims to exploit ×37 reduced emittance 2.0 storage ring, consists toroidal mirror close source harvest large solid angle bending beam, horizontal 1:2 focusing...
Native single-wavelength anomalous dispersion (SAD) is an attractive experimental phasing technique as it exploits weak signals from intrinsic light scatterers (Z < 20). The signal of sulfur in particular, enhanced at long wavelengths, however the absorption diffracted X-rays owing to crystal, sample support and air affects recorded intensities. Thereby, optimal measurable primarily depend on counterplay scattering factor a given X-ray wavelength. Here, benefit using wavelength 2.7 over 1.9...
Formation of highly symmetric skeletal elements in demosponges, called spicules, follows a unique biomineralization mechanism which polycondensation an inherently disordered amorphous silica is guided by ordered proteinaceous scaffold, the axial filament. The enzymatically active proteins, silicateins, are assembled into slender hybrid silica/protein crystalline superstructure that directs morphogenesis spicules. Furthermore, silicateins known to catalyze formation large variety other...
Tropinone reductase-II (TR-II) catalyzes the NADPH-dependent reduction of carbonyl group tropinone to a beta-hydroxyl group. The crystal structure TR-II complexed with NADP+ and pseudotropine (psi-tropine) has been determined at 1.9 A resolution. seven-residue peptide near active site, disordered in unliganded structure, is fixed ternary complex by participation cofactor substrate binding. psi-tropine molecule bound an orientation which satisfies product configuration stereochemical...
A new diffractometer for microcrystallography has been developed the three macromolecular crystallography beamlines of Swiss Light Source. Building upon and critically extending previous developments realised high-resolution endstations two undulator X06SA X10SA, as well super-bend dipole beamline X06DA, was designed to following core design goals. (i) Redesign goniometer a sub-micrometer peak-to-peak cylinder confusion horizontal single axis. Crystal sizes down at least 5 µm advanced...
Higher throughput has been ever demanded in the state-of-the-art protein crystallography beamlines for applications such as screening of drug targets protein-ligand complex structures at room temperature. As a potential method to achieve an order magnitude higher throughput, we explore capturing ejected droplets by acoustic levitator remotely load single crystal samples levitation diffractometer Swiss Light Source synchrotron facility. The results from X-ray diffraction experiments support...
The positional stability of acoustically levitated droplets in air and the settling time droplet positions on loading are important for precision applications acoustic levitation. We therefore study their dependence ultrasound pressure with diameters 0.01–0.2 times wavelength a single-axis levitator operating at 39 kHz. find that observed resonance frequencies agree well theory. However, damping coefficients oscillations exhibit large anisotropy, size deviates greatly from behavior expected...