A5069447749- Developmental Biology and Gene Regulation
- Hippo pathway signaling and YAP/TAZ
- Neurobiology and Insect Physiology Research
- Invertebrate Immune Response Mechanisms
- Kruppel-like factors research
- Genetics, Aging, and Longevity in Model Organisms
- Single-cell and spatial transcriptomics
- Biological and pharmacological studies of plants
- CRISPR and Genetic Engineering
- Bioactive Compounds in Plants
- Silk-based biomaterials and applications
- Pluripotent Stem Cells Research
- Marine Biology and Environmental Chemistry
- Plant Molecular Biology Research
- Wnt/β-catenin signaling in development and cancer
- Freshwater macroinvertebrate diversity and ecology
- Physiological and biochemical adaptations
University of Virginia
2024
University of California, Berkeley
2009-2022
Two types of information are particularly valuable in understanding the development a tissue or an organ from small population founder cells. First, it is useful to know composition final structure terms contribution individual Second, important understand cell-cell interactions. To facilitate study both these aspects at tissue-wide level, we have developed method, TIE-DYE, that allows simultaneous lineage tracing multiple cell populations as well genetic manipulation subset populations....
In both vertebrates and invertebrates, generating a functional appendage requires interactions between ectoderm-derived epithelia mesoderm-derived cells. To investigate such interactions, we used single-cell transcriptomics to generate temporal cell atlas of the Drosophila wing disc from two developmental time points. Using these data, visualized gene expression using multilayered model cataloged ligand–receptor pairs that could mediate signaling epithelial cells adult muscle precursors...
An important unanswered question in regenerative biology is to what extent regeneration accomplished by the reactivation of gene regulatory networks used during development versus activation regeneration-specific transcriptional programs. Following damage, Drosophila imaginal discs, larval precursors adult structures, can regenerate missing portions localized proliferation damage-adjacent tissue. Using single-cell transcriptomics regenerating wing we have obtained a comprehensive view...
Regeneration following tissue damage often necessitates a mechanism for cellular re-programming, so that surviving cells can give rise to all cell types originally found in the damaged tissue. This process, if unchecked, also generate are inappropriate given location. We conducted screen genes negatively regulate frequency of notum-to-wing transformations genetic ablation and regeneration wing pouch, from which we identified mutations transcriptional co-repressor C-terminal Binding Protein...
Abstract Multicellular organisms require strict growth control mechanisms to ensure that an organ reaches, but does not grossly exceed, its appropriate size and shape. In unbiased mosaic screen for genes involved in regulation, we identified a loss-of-function allele of the gene CtBP conferred advantage homozygous mutant tissue. encodes transcriptional co-repressor found diverse organisms, yet role regulating tissue is known. We functions as negative regulator by restricting expression...
Abstract The ability to induce cell death in a controlled stereotypic manner has led the discovery of evolutionary conserved molecules and signaling pathways necessary for tissue growth, repair, regeneration. Here we report development new method genetically Drosophila . This advantages over other current methods relies on expression E. coli enzyme Nitroreductase (NTR) with exogenous application nitroimidazole prodrug, Ronidazole. NTR is spatially using GAL4/UAS system while temporal control...
Abstract Whether regeneration is primarily accomplished by re-activating gene regulatory networks used previously during development or activating novel regeneration-specific transcriptional programs remains a longstanding question. Currently, most genes implicated in also function development. Using single-cell transcriptomics regenerating Drosophila wing discs, we identified two cell populations within the blastema. They are each composed of cells that upregulate multiple encoding secreted...
Abstract In both vertebrates and invertebrates, generating a functional appendage requires interactions between ectoderm-derived epithelia mesoderm-derived cells. To investigate such interactions, we used single-cell transcriptomics to generate cell atlas of the Drosophila wing disc at two time points during development. Using these data, gene expression using multi-layered model catalogued ligand-receptor pairs that could mediate signaling epithelial cells adult muscle precursors (AMPs). We...