Cell polarity must be integrated with tissue for proper development. The Drosophila embryonic central nervous system (CNS) is a highly polarized tissue; neuroblasts occupy the most apical layer of cells within CNS, and lie just basal to neural epithelium. Neuroblasts are CNS progenitor undergo multiple rounds asymmetric cell division, ;budding off' smaller daughter (GMCs) from side opposite epithelium, thereby positioning neuronal/glial progeny towards embryo interior. It unknown whether...

Dietary nutrients provide macromolecules necessary for organism growth and development. In response to animal feeding, evolutionarily conserved signaling pathways are activated, leading increased rates of cell proliferation tissue growth. It remains unclear how different types within developing tissues coordinate in dietary whether coordinated is proper function. Using the early Drosophila larval brain, we asked nutrient-dependent neural stem cells (neuroblasts), glia, trachea among these...

The mechanisms that generate neural diversity during development remains largely unknown. Here, we use scRNA-seq methodology to discover new features of the Drosophila larval CNS across several key developmental timepoints. We identify multiple progenitor subtypes - both stem cell-like neuroblasts and intermediate progenitors change gene expression development, report on candidate markers for each class progenitors. a pool quiescent in newly hatched larvae show they are transcriptionally...

The LIM domain defines a zinc-binding motif found in growing number of eukaryotic proteins that regulate cell growth and differentiation during development. Members the cysteine-rich protein (CRP) family have been implicated muscle vertebrates. Here we report identification characterization cDNA clones encoding two members CRP Drosophila, referred to as (Mlp). Mlp60A encodes with single linked glycine-rich region. Mlp84B five tandem LIM-glycine modules. In embryo, Mlp gene expression is...

Stem cells enter and exit quiescence as part of normal developmental programs to maintain tissue homeostasis in adulthood. Although it is clear that stem cell intrinsic extrinsic cues, local systemic, regulate quiescence, remains unclear whether cues coordinate control how cue coordination achieved. Here, we report Notch signaling coordinates neuroblast temporal with nutrient Drosophila. When activity reduced, delayed or altogether bypassed, some neuroblasts dividing continuously during the...

Abstract The neurogenic period, where neural stem cells (NSCs) proliferate to produce molecularly distinct progeny in the developing brain, is a critical time of growth many organisms. Proper brain development crucial for survival and requires strict regulation NSC divisions along set developmental timeline. In Drosophila NSCs known as neuroblasts (NBs), cell intrinsic programs integrate with extrinsic cues control periods rapid through temporal patterning genes. Without regulation, can...

Cell proliferation is coupled with nutrient availability. If nutrients become limited, ceases, because growth factor and/or PI3-kinase activity levels attenuated. Here, we report an exception to this generality within a subpopulation of Drosophila neural stem cells (neuroblasts). We find that most neuroblasts enter and exit cell cycle in nutrient-dependent manner reversible regulated by PI3-kinase. However, small subset, the mushroom body neuroblasts, which generate neurons important for...

Correct positioning of stem cells within their niche is essential for tissue morphogenesis and homeostasis. Yet how acquire maintain position remains largely unknown. Here, we show that a subset brain neuroblasts (NBs) in Drosophila utilize PI3-kinase DE-cadherin to build adhesive contact NB positioning. NBs remain native microenvironment when levels activity are elevated NBs. This occurs through dependent regulation DE-Cadherin mediated cell adhesion between neighboring cortex glia, GMC...

Neuroblasts in Drosophila divide asymmetrically, sequentially expressing a series of intrinsic factors to generate diversity neuron types. These known as temporal dictate timing neuroblast transitions response steroid hormone signaling and specify early versus late fates progeny. After completing their programs, neuroblasts differentiate or die, finalizing both number type within each lineage. From screen aimed at identifying genes required terminate divisions, we identified Notch pathway...

Neuroblasts in Drosophila divide asymmetrically, sequentially expressing a series of intrinsic factors to generate diversity neuron types. These known as temporal dictate timing neuroblast transitions response steroid hormone signaling and specify early versus late fates progeny. After completing their programs, neuroblasts differentiate or die, finalizing both number type within each lineage. From screen aimed at identifying genes required terminate divisions, we identified Notch pathway...

Correct positioning of stem cells within their niche is essential for tissue morphogenesis and homeostasis. How acquire maintain position remains largely unknown. Here, we show that a subset brain neuroblasts (NBs) in Drosophila utilize Phosphoinositide 3-kinase (PI3-kinase) DE-cadherin to build adhesive contact NB positioning. NBs remain native microenvironment when levels PI3-kinase activity are elevated NBs. This occurs through PI3-kinase-dependent regulation DE-Cadherin-mediated cell...

Abstract The ability to induce cell death in a controlled stereotypic manner has led the discovery of evolutionary conserved molecules and signaling pathways necessary for tissue growth, repair, regeneration. Here we report development new method genetically Drosophila . This advantages over other current methods relies on expression E. coli enzyme Nitroreductase (NTR) with exogenous application nitroimidazole prodrug, Ronidazole. NTR is spatially using GAL4/UAS system while temporal control...

Neuroblasts in Drosophila divide asymmetrically, sequentially expressing a series of intrinsic factors to generate diversity neuron types. These known as temporal dictate timing neuroblast transitions response steroid hormone signaling and specify early versus late fates progeny. After completing their programs, neuroblasts differentiate or die, finalizing both number type within each lineage. From screen aimed at identifying genes required terminate divisions, we identified Notch pathway...

Neuroblasts in Drosophila divide asymmetrically, sequentially expressing a series of intrinsic factors to generate diversity neuron types. These known as temporal dictate timing neuroblast transitions response steroid hormone signaling and specify early versus late fates progeny. After completing their programs, neuroblasts differentiate or die, finalizing both number type within each lineage. From screen aimed at identifying genes required terminate divisions, we identified Notch pathway...