A5001890514- Neurobiology and Insect Physiology Research
- Developmental Biology and Gene Regulation
- Invertebrate Immune Response Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
University of Virginia
2023-2024
Abstract The neurogenic period, where neural stem cells (NSCs) proliferate to produce molecularly distinct progeny in the developing brain, is a critical time of growth many organisms. Proper brain development crucial for survival and requires strict regulation NSC divisions along set developmental timeline. In Drosophila NSCs known as neuroblasts (NBs), cell intrinsic programs integrate with extrinsic cues control periods rapid through temporal patterning genes. Without regulation, can...
Neuroblasts in Drosophila divide asymmetrically, sequentially expressing a series of intrinsic factors to generate diversity neuron types. These known as temporal dictate timing neuroblast transitions response steroid hormone signaling and specify early versus late fates progeny. After completing their programs, neuroblasts differentiate or die, finalizing both number type within each lineage. From screen aimed at identifying genes required terminate divisions, we identified Notch pathway...
Neuroblasts in Drosophila divide asymmetrically, sequentially expressing a series of intrinsic factors to generate diversity neuron types. These known as temporal dictate timing neuroblast transitions response steroid hormone signaling and specify early versus late fates progeny. After completing their programs, neuroblasts differentiate or die, finalizing both number type within each lineage. From screen aimed at identifying genes required terminate divisions, we identified Notch pathway...
Neuroblasts in Drosophila divide asymmetrically, sequentially expressing a series of intrinsic factors to generate diversity neuron types. These known as temporal dictate timing neuroblast transitions response steroid hormone signaling and specify early versus late fates progeny. After completing their programs, neuroblasts differentiate or die, finalizing both number type within each lineage. From screen aimed at identifying genes required terminate divisions, we identified Notch pathway...
Neuroblasts in Drosophila divide asymmetrically, sequentially expressing a series of intrinsic factors to generate diversity neuron types. These known as temporal dictate timing neuroblast transitions response steroid hormone signaling and specify early versus late fates progeny. After completing their programs, neuroblasts differentiate or die, finalizing both number type within each lineage. From screen aimed at identifying genes required terminate divisions, we identified Notch pathway...
Genome manipulation methods in C. elegans require microinjecting DNA or ribonucleoprotein complexes into the microscopic core of gonadal syncytium. These microinjections are technically demanding and represent a key bottleneck for all genome engineering transgenic approaches elegans. While there have been steady improvements ease efficiency genetic manipulation, not comparable advances physical process microinjection. Here, we report simple inexpensive method handling worms using paintbrush...
Genome manipulation methods in
Summary Neuroblasts in Drosophila divide asymmetrically, sequentially expressing a series of intrinsic factors to generate diversity neuron types. These known as temporal dictate timing neuroblast transitions response steroid hormone signaling and specify early versus late fates progeny. After completing their programs, neuroblasts differentiate or die, finalizing both number type within each lineage. From screen aimed at identifying genes required terminate divisions, we identified Notch...