A5029040324- Immune Cell Function and Interaction
- interferon and immune responses
- SARS-CoV-2 and COVID-19 Research
- Immune responses and vaccinations
- Chronic Lymphocytic Leukemia Research
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Acute Lymphoblastic Leukemia research
- Aortic aneurysm repair treatments
- Aortic Disease and Treatment Approaches
- RNA modifications and cancer
- MicroRNA in disease regulation
- Developmental Biology and Gene Regulation
- Genetic Associations and Epidemiology
- CRISPR and Genetic Engineering
- Macrophage Migration Inhibitory Factor
- Genetics and Neurodevelopmental Disorders
- Single-cell and spatial transcriptomics
- Cellular Mechanics and Interactions
- Pregnancy and preeclampsia studies
- Circular RNAs in diseases
- Plant Molecular Biology Research
- Neurobiology and Insect Physiology Research
- Pluripotent Stem Cells Research
- Wnt/β-catenin signaling in development and cancer
Children's Hospital of Philadelphia
2020-2024
University of Pennsylvania
2023-2024
University of Virginia
2013-2023
Institute for Stem Cell Biology and Regenerative Medicine
2023
Geisinger Health System
2015
Susquehanna University
2012-2013
Geisinger Medical Center
2013
Abstract Background Abdominal aortic aneurysm (AAA) is a dilatation of the aorta affecting most frequently elderly men. Histologically AAAs are characterized by inflammation, vascular smooth muscle cell apoptosis, and extracellular matrix degradation. The mechanisms AAA formation, progression, rupture currently poorly understood. A previous mRNA expression study revealed large number differentially expressed genes between non-aneurysmal control aortas. MicroRNAs (miRNAs), small non-coding...
Abdominal aortic aneurysm (AAA), a dilatation of the infrarenal aorta, typically affects males >65 years. The pathobiological mechanisms human AAA are poorly understood. goal this study was to identify novel pathways involved in development AAAs.A custom-designed 'AAA-chip' used assay 43 differentially expressed genes identified previously published microarray between (n = 15) and control abdominal aorta. Protein analyses were performed on selected genes.Altogether 38 showed significantly...
We investigated transcriptional control of gene expression in human abdominal aortic aneurysm (AAA). previously identified 3274 differentially expressed genes AAA tissue compared to non-aneurysmal controls. Four transcription factors (ELF1, ETS2, STAT5 and RUNX1) were selected for genome-wide chromatin immunoprecipitation. Transcription factor binding was enriched 4760 distinct (FDR < 0.05), which 713 AAA. Functional classification using Gene Ontology (GO), KEGG, Network Analysis revealed...
Ikaros is a transcriptional factor required for conventional T cell development, differentiation, and anergy. While the related factors Helios Eos have defined roles in regulatory cells (Treg), role has not been established. To determine function of Treg lineage, we generated mice with Treg-specific deletion gene ( Ikzf1 ). We find that cooperates Foxp3 to establish major portion epigenome transcriptome. Ikaros-deficient exhibit Th1-like expression abnormal production IL-2, IFNg, TNFa,...
Genome-wide-association studies (GWASs) have discovered genetic signals robustly associated with BMD, but typically not the precise localization of effector genes. By intersecting genome-wide promoter-focused Capture C and assay for transposase-accessible chromatin using sequencing (ATAC-seq) data generated in human mesenchymal progenitor cell (hMSC)-derived osteoblasts, consistent contacts were previously predicted between
Correct positioning of stem cells within their niche is essential for tissue morphogenesis and homeostasis. How acquire maintain position remains largely unknown. Here, we show that a subset brain neuroblasts (NBs) in Drosophila utilize Phosphoinositide 3-kinase (PI3-kinase) DE-cadherin to build adhesive contact NB positioning. NBs remain native microenvironment when levels PI3-kinase activity are elevated NBs. This occurs through PI3-kinase-dependent regulation DE-Cadherin-mediated cell...
Ikaros is a transcriptional factor required for conventional T cell development, differentiation, and anergy. While the related factors Helios Eos have defined roles in regulatory cells (Treg), role has not been established. To determine function of Treg lineage, we generated mice with Treg-specific deletion gene ( Ikzf1 ). We find that cooperates Foxp3 to establish major portion epigenome transcriptome. Ikaros-deficient exhibit Th1-like expression abnormal IL-2, IFNg, TNFa, involved Wnt...
Abstract Ikaros is a transcriptional factor required for conventional T cell development, differentiation, and anergy. While the related factors Helios Eos have defined roles in regulatory cells (Treg), role has not been established. To determine function of Treg lineage, we generated mice with Treg-specific deletion gene ( Ikzf1 ). We find that cooperates Foxp3 to establish major portion epigenome transcriptome. Ikaros-deficient exhibit Th1-like expression abnormal IL-2, IFNg, TNFa,...
ABSTRACT Although genome wide association studies (GWAS) have been crucial for the identification of loci associated with sleep traits and disorders, method itself does not directly uncover underlying causal variants corresponding effector genes. The overwhelming majority such reside in non-coding regions are therefore presumed to impact activity cis -regulatory elements, as enhancers. Our previously reported ‘variant-to-gene mapping’ effort human induced pluripotent stem cell (iPSC)-derived...
The prevalence of childhood obesity is increasing worldwide, along with the associated common comorbidities type 2 diabetes and cardiovascular disease in later life. Motivated by evidence for a strong genetic component, our prior genome-wide association study (GWAS) efforts revealed 19 independent signals trait; however, mechanism action these loci remains to be elucidated. To molecularly characterize we sought determine underlying causal variants corresponding effector genes within diverse...
Ikaros is a transcriptional factor required for conventional T cell development, differentiation, and anergy. While the related factors Helios Eos have defined roles in regulatory cells (Treg), role has not been established. To determine function of Treg lineage, we generated mice with Treg-specific deletion gene ( Ikzf1 ). We find that cooperates Foxp3 to establish major portion epigenome transcriptome. Ikaros-deficient exhibit Th1-like expression abnormal production IL-2, IFNg, TNFa,...
Summary Neuroblasts in Drosophila divide asymmetrically, sequentially expressing a series of intrinsic factors to generate diversity neuron types. These known as temporal dictate timing neuroblast transitions response steroid hormone signaling and specify early versus late fates progeny. After completing their programs, neuroblasts differentiate or die, finalizing both number type within each lineage. From screen aimed at identifying genes required terminate divisions, we identified Notch...
Abstract Genome-wide association studies (GWAS) have identified hundreds of genetic loci associated with autoimmune disease, but the causal variants and their contribution to immune dysregulation remain largely unknown. We measured dynamics chromosome conformation, chromatin accessibility, gene transcription across three phases naive human CD4+ T cell activation co-localized active cis-regulatory elements 95% credible set from 15 GWAS. Reorganization structure placed these in direct contact...
Ikaros is a transcriptional factor required for conventional T cell development, differentiation, and anergy. While the related factors Helios Eos have defined roles in regulatory cells (Treg), role has not been established. To determine function of Treg lineage, we generated mice with Treg-specific deletion gene (Ikzf1). We find that cooperates Foxp3 to establish major portion epigenome transcriptome. Ikaros-deficient exhibit Th1-like expression abnormal IL-2, IFNg, TNFa, involved Wnt Notch...
SUMMARY The ch12q13 obesity locus is among the most significant childhood loci identified in genome-wide association studies. This resides a non-coding region within FAIM2 ; thus, underlying causal variant(s) presumably influence disease susceptibility via an on cis -regulation genomic region. We implicated rs7132908 as putative variant at this leveraging combination of our inhouse 3D data, public domain datasets, and several computational approaches. Using luciferase reporter assay human...
Ikaros is a transcriptional factor required for conventional T cell development, differentiation, and anergy. While the related factors Helios Eos have defined roles in regulatory cells (Treg), role has not been established. To determine function of Treg lineage, we generated mice with Treg-specific deletion gene ( Ikzf1 ). We find that cooperates Foxp3 to establish major portion epigenome transcriptome. Ikaros-deficient exhibit Th1-like expression abnormal IL-2, IFNg, TNFa, involved Wnt...