A5014156000- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Developmental Biology and Gene Regulation
- Genomics and Chromatin Dynamics
- Bacteriophages and microbial interactions
- DNA and Nucleic Acid Chemistry
- Bacterial Genetics and Biotechnology
- Nitrogen and Sulfur Effects on Brassica
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cancer-related Molecular Pathways
- Neurobiology and Insect Physiology Research
- Physiological and biochemical adaptations
- DNA Repair Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Reformation and Early Modern Christianity
- Plant pathogens and resistance mechanisms
- RNA regulation and disease
- Silkworms and Sericulture Research
- Cancer-related gene regulation
- Silk-based biomaterials and applications
- Heat shock proteins research
- 14-3-3 protein interactions
The Ohio State University
2009-2025
Duke University
2006-2009
Duke University Hospital
1999-2008
Duke Medical Center
1999-2008
Howard Hughes Medical Institute
1999-2008
Columbia University
1987-1991
Harvard University
1984-1988
Harvard University Press
1988
Yale University
1981
Junichiro Sonoda and Robin P. Wharton Howard Hughes Medical Institute (HHMI), Department of Genetics, Duke University Center, Durham, North Carolina 27710 USA
The Drosophila brain tumor (brat) gene encodes a member of the conserved NHL family proteins, which appear to regulate differentiation and growth in variety organisms. One founding members, Caenorhabditis elegans LIN-41, is thought control posttranscriptional expression. However, mechanism by or any other protein, acts has not been clear. Using yeast "four-hybrid" interaction assay, we show that Brain Tumor recruited hunchback (hb) mRNA through interactions with Nanos Pumilio, bind RNA...
In the Drosophila embryo, Nanos and Pumilio collaborate to repress translation of hunchback mRNA in somatic cytoplasm. Both proteins are also required for repression maternal Cyclin B germline; it has not been clear whether they act directly on mRNA, if so, regulation presumptive germline cytoplasm proceeds by similar or fundamentally different mechanisms. this report, we show that bind an element 3' UTR mRNA. Regulation differ two significant respects. First, is dispensable (but hunchback)...
Abdominal segmentation in the Drosophila embryo is governed by a gradient of Nanos (Nos) emanating from posterior pole. This derived translation nos mRNA that localized pole plasm; contrast, unlocalized translationally repressed. Here we define essential signals 3' untranslated region (UTR) mRNA. Deletion 184-nucleotide translational control element (TCE) UTR leads to derepression bulk cytoplasm and development lethal anterior defects. Furthermore, minimal containing essentially only TCE its...
Previous experiments suggest that a key event in the commitment of cultured mammalian cells to entering S phase is rise activity transcription factor E2F. In this report, we study role Drosophila E2F imaginal disc vivo, by examining distribution endogenous protein and studying consequences ectopic expression. First, find E217 falls from high very low levels as initiate DNA synthesis during developmentally regulated G1-S-transition eye disc. Second, expression drives many otherwise quiescent...
The Brain Tumor (Brat) protein is recruited to the 3′ untranslated region (UTR) of hunchback mRNA regulate its translation. Recruitment mediated by interactions between Pumilio RNA-binding Puf repeats and NHL domain Brat, a conserved structural motif present in large family growth regulators. In this report, we describe crystal structure Brat model Pumilio–Brat complex derived from silico docking experiments supported mutational analysis protein–protein interface. A key feature recognition...
The Drosophila Pumilio (Pum) and Nanos (Nos) RNA-binding proteins govern abdominal segmentation in the early embryo, as well a variety of other events during development. They bind together to compound Response Element (NRE) present thousands maternal mRNAs ovary including hunchback ( hb ) mRNA, thereby regulating poly-adenylation, translation, stability. Many studies support model which mRNA recognition effector recruitment are carried out by distinct regions each protein. well-ordered Pum...
ABSTRACT Nanos (Nos) is a translational regulator that governs abdominal segmentation of the Drosophila embryo in collaboration with Pumilio (Pum). In embryo, mode Nos and Pum action clear: they form ternary complex critical sequences 3′UTR hunchback mRNA to regulate its translation. also regulates germ cell development survival ovary. While this aspect biological activity appears be evolutionarily conserved, process not yet well understood. report, we show interacts Cup, which required for...
Puf domain proteins bind specific sequences in mRNAs to regulate their translation or stability, both. Neither the mechanism of action nor identities targeted have been well defined. Recent work suggests that generally act by recruiting Pop2, a deadenylation enzyme is part large complex. from separate group defines subset Drosophila transcriptome bound fly protein, Pumilio. Together, these papers substantially increase our understanding biology family mRNA regulators.