A5051727817- DNA and Nucleic Acid Chemistry
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- HIV/AIDS drug development and treatment
- Biochemical and Molecular Research
- Quinazolinone synthesis and applications
- Extracellular vesicles in disease
- Advanced NMR Techniques and Applications
- Amino Acid Enzymes and Metabolism
- Solid-state spectroscopy and crystallography
- RNA Interference and Gene Delivery
- Microbial Natural Products and Biosynthesis
- Synthesis and biological activity
- Multicomponent Synthesis of Heterocycles
- Autophagy in Disease and Therapy
- Enzyme Catalysis and Immobilization
- Bacterial Genetics and Biotechnology
- RNA modifications and cancer
- Microbial Metabolites in Food Biotechnology
- Synthetic Organic Chemistry Methods
- Nuclear Physics and Applications
- Enzyme Production and Characterization
- Drug Transport and Resistance Mechanisms
Universidade do Porto
2018-2024
Rede de Química e Tecnologia
2018-2019
Fundação Faculdade de Medicina
2019
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2018
Centro Interdisciplinar de Investigação Marinha e Ambiental
2017
PLP-dependent enzymes described on this review are attractive targets for enzyme engineering towards their application in an industrial biotechnology framework.
Many fungal quinazolinone metabolites, which contain the methyl-indole pyrazino [1,2-b]quinazoline-3,6-dione core, have been found to possess promising antitumor activity. The purpose of this work was synthesize enantiomeric pairs two members family, explore their potential as and ability revert multidrug resistance. marine natural product fiscalin B (4c), antienantiomers (4b, 5b, 5c) were synthesized via a one-pot approach, while syn enantiomers (4a, 4d, 5a, 5d) synthetized by multi-step...
For the first time, an aptamer that specifically binds to cathepsin B (CatB)-overexpressing prostate cancer cells was identified by combining SELEX and computational tools.
Non-B DNA conformations are molecular structures that do not follow the canonical double helix. Mutagenetic instability in nuclear and mitochondrial (mtDNA) genomes has been associated with simple non-B conformations, as hairpins or more complex structures, G-quadruplexes. One of these is Structure A, a cloverleaf-like conformation predicted for 93-nt (nucleotide) stretch mtDNA control region 5′-peripheral domain. A embedded hot spot 3′ end human deletions revealing its importance...
The catalytic mechanism of Pdx2 was studied with atomic detail employing the computational ONIOM hybrid QM/MM methodology. employs a Cys-His-Glu triad to deaminate glutamine glutamate and ammonia - source nitrogen pyridoxal 5'-phosphate (PLP). This enzyme is, therefore, rate-limiting step in PLP biosynthetic pathway Malaria Tuberculosis pathogens that rely on this obtain PLP. For reason, is considered novel promising drug target treat these diseases. results obtained show occurs six steps...
Aim: The aim of this work was to synthesize fiscalin B, pursuit the development a library derivatives and investigate for their potential antitumor activity. Introduction: Marine organisms provided numerous novel compounds with sensational multiple pharmacological properties. necessity therapeutics has gain more importance especially because resistance associated current inexistent treatments incurable diseases. Fiscalin B is fungal metabolite pyrazino[2,1-b]quinazoline-3,6-dione system...
Pyridoxal 5'-phosphate (PLP), the active form of vitamin B6, is an essential cofactor required by more than 160 families enzymes. Its role as electron sink makes it imperative for catalysis a myriad chemical reactions. Contrarily to microorganisms and plants, humans other mammals are not able synthesize PLP de novo, resorting "salvage pathway" that helps maintain homeostasis [1]. The correct functioning this salvage pathway crucial cell, demonstrated correlation between low levels occurrence...
An atomistic description of Pdx2 catalytic mechanism is provided by QM/MM methodologies. Pdx2, the glutaminase subunit pyridoxal 5’-phosphate (PLP) synthase, characterized a Cys-His-Glu triad used to deaminate glutamine glutamate. While being absent in humans, essential for development and replication protozoan Plasmodium falciparum Mycobacterium tuberculosis pathogens, which are responsible two deadly diseases, malaria tuberculosis, respectively. thus promising drug target fighting these...