A5006611590- Phytochemicals and Medicinal Plants
- MicroRNA in disease regulation
- Cytokine Signaling Pathways and Interactions
- Immunotherapy and Immune Responses
- Extracellular vesicles in disease
- HIV Research and Treatment
- Heat shock proteins research
- Animal Virus Infections Studies
- Viral Infections and Outbreaks Research
- Medicinal Plants and Neuroprotection
- SARS-CoV-2 and COVID-19 Research
- Spaceflight effects on biology
- COVID-19 epidemiological studies
- vaccines and immunoinformatics approaches
- Inhalation and Respiratory Drug Delivery
- Viral gastroenteritis research and epidemiology
- Pharmacological Effects of Medicinal Plants
- Hepatitis C virus research
- Influenza Virus Research Studies
- Hepatitis B Virus Studies
- Computational Drug Discovery Methods
- Cancer Immunotherapy and Biomarkers
- Cell death mechanisms and regulation
- Protein Structure and Dynamics
- Immune cells in cancer
University of Michigan
2012-2020
BioSurfaces (United States)
2017-2020
Michigan United
2016
Ann Arbor Center for Independent Living
2014
University of Kansas Medical Center
2012
Resident alveolar macrophages (AMs) suppress allergic inflammation in murine asthma models. Previously we reported that resident AMs can blunt inflammatory signaling epithelial cells (ECs) by transcellular delivery of suppressor cytokine 3 (SOCS3) within extracellular vesicles (EVs). Here examined the role vesicular SOCS3 secretion as a mechanism which restrain responses airway ECs. Bronchoalveolar lavage fluid (BALF) levels were reduced asthmatics and allergen-challenged mice. Ex vivo was...
Lung cancer remains the leading cause of cancer-related death in United States. Although alveolar macrophage (AM) comprises major resident immune cell lung, few studies have investigated its role lung development. We recently discovered a potentially novel mechanism wherein AMs regulate STAT-induced inflammatory responses neighboring epithelial cells (ECs) via secretion and delivery suppressors cytokine signaling 3 (SOCS3) within extracellular vesicles (EVs). Here, we explored impact SOCS3...
1,2,3-Triazole side chain containing coumarin and biphenyl amides were designed, synthesized evaluated as potent Hsp90 C-terminal inhibitors, which exhibited improved anti-proliferative activities against a panel of cancer cell lines.
Elicitation of neutralizing antibody responses against hepatitis C virus (HCV) has been a challenging goal. While the E2 subunit HCV envelope glycoprotein complex is promising target for generating cross-genotype antibodies, vaccinations with soluble immunogens generally induce weak responses. Here, (i.e., E2.661 and E2c.661) were loaded into lipid-based nanovaccines examined induction Compared immunogens, nanoparticles elicited 6- to 20-fold higher E2-specific serum IgG titers in mice....
A new withanolide, named withawrightolide (1), and four known withanolides (2–5) were isolated from the aerial parts of Datura wrightii. The structure compound 1 was elucidated through 2D NMR other spectroscopic techniques. In addition, withametelin L (2) confirmed by X-ray crystallographic analysis. Using MTS viability assays, 1–5 showed antiproliferative activities against human glioblastoma (U251 U87), head neck squamous cell carcinoma (MDA-1986), normal fetal lung fibroblast (MRC-5)...
In patients with triple-negative breast cancer (TNBC), evidence suggests that tumor-initiating cells (TIC) have stem cell-like properties, leading to invasion and metastasis. HSP90 plays a critical role in the conformational maintenance of many client proteins TIC development. Therefore, we hypothesize novel C-terminal inhibitors KU711 KU758 can target represent promising strategy for overcoming Human (MDA-MB-468LN, MDA-MB-231) treated KU711, KU758, 17-AAG showed 50-80% decrease markers CD44...
The natural product withaferin A exhibits potent antitumor activity and other diverse pharmacological activities. recently discovered withalongolide A, a C-19 hydroxylated congener of was reported to possess cytotoxic against head neck squamous cell carcinomas. Semisynthetic acetylated analogues were shown be considerably more than the parent compound. To further explore structure-activity relationships, 20 new semisynthetic synthesized evaluated for four different cancer lines. number...
A new withanolide 1 named withawrightolide, and four known withanolides 2-5 were isolated from the aerial parts of Datura wrightii Regel (Solanaceae). The structure compound was elucidated through 2D NMR other spectroscopic techniques. In addition, withametelin L 2 confirmed by X-ray crystallographic analysis. Using MTS viability assays, 1-5 showed antiproliferative activities against human glioblastoma (U251 U87), head neck squamous cell carcinoma (MDA-1986), normal fetal lung fibroblast...
Abstract The recent outbreaks of Ebola virus in Africa and isolated incidents the U.S. have increased global attention concern for this pathogen. highly contagious nature severe mortality rate infection highlight urgent need an effective vaccine. To cull its spread, many vaccines are currently being tested pre-clinical clinical settings. Here, we report development a vaccine nanoparticle system that can safely elicit antibody responses protect animals against viral challenge. We incorporated...
Abstract Background: Current adjuvant strategies for an adrenocortical carcinoma (ACC) using mitotane or multidrug cytotoxics as in the Italian protocol have limited efficacy yet carry significant morbidity and toxicity. We recently demonstrated that novel natural withanolides from North American Physalis plant potently selectively inhibit several solid tumors including ACCs vitro. These anticancer mechanisms through a combination of oxidative stress heat shock protein client modulation...
OBJECTIVES/SPECIFIC AIMS: Insufficient endogenous expression of suppressor cytokine signaling 3 (SOCS3) with subsequent over-activation its target, the transcription factor STAT3, has been associated tumorigenesis and cancer development in lung other organs. We have observed that a “backup” source SOCS3 lung, namely secreted microvesicles (MVs) by alveolar macrophages, is reduced bronchoalveolar lavage fluid (BALF) KRAS mutant mice harboring tumors. Here we sought to evaluate levels BALF...
Abstract Rationale: Inadequate expression of suppressor cytokine signaling 3 (SOCS3) with subsequent activation its target, the transcription factor STAT3, has been implicated in tumorigenesis and cancer progression lung other organs. Our lab recently reported novel capability alveolar macrophages (AMs) to secrete SOCS3 within microvesicles (MVs). While AM delivery MV-encapsulated was shown suppress inflammatory recipient epithelial cells, potential significance this process restraining...