Abstract Retinal prostheses have been developed to fight blindness in people affected by outer retinal layer dystrophies. To date, few hundred patients received a implant. Inspired intraocular lenses, we designed foldable and photovoltaic wide-field epiretinal prosthesis (named POLYRETINA) capable of stimulating wireless ganglion cells. Here show that within visual angle 46.3 degrees, POLYRETINA embeds 2215 pixels, which 967 are the central area 5 mm, it is allow implantation through small...

Abstract Retinal prostheses hold the promise of restoring vision in totally blind people. However, a decade clinical trials highlighted quantitative limitations hampering possibility reaching this goal. A key challenge retinal stimulation is to independently activate neurons over large portion subject’s visual field. Reaching such goal would significantly improve perception accuracy implants’ users, along with their spatial cognition, attention, ambient mapping and interaction environment....

Abstract Organic materials, such as conjugated polymers, are attractive building blocks for bioelectronic interfaces. In particular, organic semiconductors show excellent performance in light-mediated excitation and silencing of neuronal cells tissues. However, the main challenges these photovoltaic interfaces compared to inorganic prostheses limited adhesion polymers aqueous environments exploitation materials responsive visible spectrum. Here, we a interface optimized stimulation...

Signal transduction downstream of axon guidance molecules is essential to steer developing axons. Second messengers including cAMP are key shared by a multitude signaling pathways and required for wide range cellular processes pathfinding. Yet, how these achieve specificity each their remains elusive. Subcellular compartmentation emerged as flexible strategy reach such specificity. Here, we show that point contact-restricted signals control ephrin-A5-evoked repulsion in vitro modulating...

Inherited retinal dystrophies are a large and heterogeneous group of degenerative diseases caused by mutations in various genes. Given the favourable anatomical immunological characteristics eye, gene therapy holds great potential for their treatment. Our goal is to validate preservation visual functions viral-free homology directed repair an autosomal recessive loss function mutation. We used tailored CRISPR/Cas9-based editing system prevent photoreceptor death Rd10 mouse model retinitis...

Photovoltaic retinal prostheses theoretically offer the possibility of stand-alone high-resolution electrical stimulation retina. However, achieving focused epiretinal is particularly challenging because axonal activation and cell coupling. Recent evidence shows that long electric pulses permit a more focal ganglion cells, non-rectangular waveforms induce higher network-mediated indirect activity.The role pulse shape in focusing underlying mechanisms are not yet fully understood.To address...

Objective.Retinal stimulation in blind patients evokes the sensation of discrete points light called phosphenes, which allows them to perform visually guided tasks, such as orientation, navigation, object recognition, manipulation and reading. However, clinical benefit artificial vision profoundly is still tenuous, several engineering biophysical obstacles keep it far away from natural perception. The relative preservation inner retinal neurons hereditary degenerative diseases, retinitis...

Objective. Temporal resolution is a key challenge in artificial vision. Several prosthetic approaches are limited by the perceptual fading of evoked phosphenes upon repeated stimulation from same electrode. Therefore, implanted patients forced to perform active scanning, via head movements, refresh visual field viewed camera. However, scanning draining task, and it crucial find compensatory strategies reduce it.Approach. To address this question, we implemented simulated vision using virtual...

Digital immersive technologies have become increasingly prominent in clinical research and practice, encompassing medical communication technical education, serious games for health, psychotherapy, interfaces neurorehabilitation. The worldwide effervescence of digital health therapeutics has prompted the development testing numerous applications interaction methods. Nevertheless, lack consistency approaches peculiarity constructed environments contribute to an increasing disparity between...

Abstract Signal transduction downstream of axon guidance molecules is essential to steer developing axons. Second messengers including cAMP are key shared by a multitude signaling pathways and required for wide range cellular processes pathfinding. Yet, how these achieve specificity each their remains elusive. Subcellular compartmentation emerged as flexible strategy reach such specificity. Here, we show that point contact-restricted signals control ephrin-A5-evoked repulsion in vitro...

Abstract Retinal prostheses hold the promise of restoring artificial vision in profoundly and totally blind people. However, a decade clinical trials highlighted quantitative limitations hampering possibility to reach this goal. A key obstacle suitable retinal stimulation is ability independently activate neurons over large portion subject’s visual field. Reaching such goal would significantly improve perception accuracy users implants, along with their spatial cognition, attention, ambient...

Abstract Objective Retinal stimulation in blind patients evokes the sensation of discrete points light called phosphenes, which allows them performing visual guided tasks, such as orientation, navigation, object recognition, manipulation and reading. However, clinical benefit artificial vision profoundly is still tenuous, several engineering biophysical obstacles keep it away from natural perception. The relative preservation inner retinal neurons hereditary degenerative diseases, retinitis...

Abstract Inherited retinal dystrophies are a large and heterogeneous group of degenerative diseases caused by mutations in various genes. Given the favourable anatomical immunological characteristics eye, gene therapy holds great potential for their treatment. We used tailored CRISPR/Cas9-based editing system to prevent photoreceptor death Rd10 mouse model retinitis pigmentosa. tested tool vitro then vivo subretinal electroporation deliver it one retinas pups at different stages...

Abstract Organic materials, such as conjugated polymers, are attractive building blocks for bioelectronic interfaces. In particular, organic semiconductors showed excellent performances in light-mediated excitation and silencing of neuronal cells tissues. However, the main challenges these photovoltaic interfaces compared to inorganic prostheses limited stability polymers aqueous environment exploitation materials only responsive visible spectrum. this report, we show a new interface...

Abstract Objective Photovoltaic retinal prostheses theoretically offer the possibility of standalone high-resolution electrical stimulation retina. However, in artificial vision, achieving locally selective epiretinal is particularly challenging, on grounds axonal activation and cell coupling. Approach Here we show that photovoltaic dystrophic circuits with capacitive-like pulses leads to a greater efficiency for indirect network-mediated ganglion cells. In addition, biophysical model inner...