A5022178993- Axon Guidance and Neuronal Signaling
- Neuroscience and Neuropharmacology Research
- Advanced biosensing and bioanalysis techniques
- Phosphodiesterase function and regulation
- Angiogenesis and VEGF in Cancer
- Retinal Development and Disorders
- Receptor Mechanisms and Signaling
- Protist diversity and phylogeny
- Neuroinflammation and Neurodegeneration Mechanisms
- Photoreceptor and optogenetics research
- Nicotinic Acetylcholine Receptors Study
- Chemical Synthesis and Analysis
- Cystic Fibrosis Research Advances
- Cellular transport and secretion
- Bioactive Compounds and Antitumor Agents
- Cell Adhesion Molecules Research
- Chemical synthesis and alkaloids
- Genetic and Kidney Cyst Diseases
- Nitric Oxide and Endothelin Effects
- Biotin and Related Studies
- Neurogenesis and neuroplasticity mechanisms
Institut de la Vision
2016-2025
Sorbonne Université
2016-2025
Inserm
2019-2025
Centre National de la Recherche Scientifique
2019-2025
Hôpital d'Enfants
2020
Maladies génétiques d’expression pédiatrique
2020
Assistance Publique – Hôpitaux de Paris
2020
Sorbonne Paris Cité
2020
Abstract The development of neuronal circuits is controlled by guidance molecules that are hypothesized to interact with the cholesterol-enriched domains plasma membrane termed lipid rafts. Whether such enable local intracellular signalling at submicrometre scale in developing neurons and required for shaping nervous system connectivity vivo remains controversial. Here, we report a role rafts generating cAMP axonal growth cones downstream ephrin-A repulsive cues. Ephrin-A-dependent...
Signal transduction downstream of axon guidance molecules is essential to steer developing axons. Second messengers including cAMP are key shared by a multitude signaling pathways and required for wide range cellular processes pathfinding. Yet, how these achieve specificity each their remains elusive. Subcellular compartmentation emerged as flexible strategy reach such specificity. Here, we show that point contact-restricted signals control ephrin-A5-evoked repulsion in vitro modulating...
cGMP is critical to a variety of cellular processes, but the available tools interfere with endogenous lack and subcellular specificity. We introduce SponGee, genetically encoded chelator this cyclic nucleotide that enables in vitro vivo manipulations single cells biochemically defined compartments. SponGee buffers physiological changes concentration various model systems while not affecting cAMP signals. provide proof-of-concept strategies by using tool highlight role signaling discrete...
Calcium is a second messenger crucial to myriad of cellular processes ranging from regulation metabolism and cell survival vesicle release motility. Current strategies directly manipulate endogenous calcium signals lack subcellular specificity. We introduce SpiCee, versatile genetically encoded chelator combining low- high-affinity sites for calcium. This scavenger enables altering signaling functions in single cells vitro vivo with biochemically controlled resolution. SpiCee paves the way...
Axonal arbors in many neuronal networks are exuberant early during development and become refined by activity-dependent competitive mechanisms. Theoretical work proposed non-competitive interactions between co-active axons to co-stabilize their connections, but the demonstration of such is lacking. Here, we provide experimental evidence that reducing cyclic AMP (cAMP) signaling a subset retinal ganglion cells favors elimination thalamic projections from neighboring neurons, pointing...
Second messengers, including cAMP, cGMP and Ca
Abstract Signal transduction downstream of axon guidance molecules is essential to steer developing axons. Second messengers including cAMP are key shared by a multitude signaling pathways and required for wide range cellular processes pathfinding. Yet, how these achieve specificity each their remains elusive. Subcellular compartmentation emerged as flexible strategy reach such specificity. Here, we show that point contact-restricted signals control ephrin-A5-evoked repulsion in vitro...
Axonal arbors in many neuronal networks are exuberant early during development and become refined by activity-dependent competitive mechanisms. Theoretical work proposed non-competitive interactions between co-active axons to co-stabilize their connections, but the demonstration of such is lacking. Here we provide experimental evidence that reducing cAMP signaling a subset retinal ganglion cells favors elimination thalamic projections from neighboring neurons, pointing cAMP-dependent...
Calcium is a second messenger crucial to myriad of cellular processes ranging from regulation metabolism and cell survival vesicle release motility. Current strategies directly manipulate endogenous calcium signals lack subcellular specificity. We developed SpiCee, versatile genetically-encoded chelator combining low high affinity sites for calcium. This scavenger enables altering signaling functions in single cells vitro vivo with biochemically-controlled resolution. SpiCee paves the way...
A bstract Second messengers, including cAMP, cGMP and Ca 2+ are often placed in an integrating position to combine the extracellular cues that orient growing axons developing brain. This view suggests axon repellents share same set of cellular messenger signals attractants evoke opposite changes. Investigating confinement these second messengers nanodomains, we instead demonstrate two repellent cues, ephrin-A5 Slit1, induce spatially segregated signals. These guidance molecules activate...
cGMP is critical to a variety of cellular processes, but the available tools interfere with endogenous lack and subcellular specificity. We introduce SponGee, genetically-encoded chelator this cyclic nucleotide that enables in vitro vivo manipulations single cells biochemically-defined compartments. SponGee buffers physiological changes concentration various model systems while not affecting cAMP signals. provide proof concept strategies using tool highlight role signaling discrete domains....