A5059332822- Neuroscience and Neuropharmacology Research
- Axon Guidance and Neuronal Signaling
- Reproductive Biology and Fertility
- Neurogenesis and neuroplasticity mechanisms
- Retinal Development and Disorders
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neural Engineering
- Renal and related cancers
- Alzheimer's disease research and treatments
- Nicotinic Acetylcholine Receptors Study
- Vestibular and auditory disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Cholinesterase and Neurodegenerative Diseases
- Retinal Imaging and Analysis
- Optical Coherence Tomography Applications
- Neuropeptides and Animal Physiology
- Zebrafish Biomedical Research Applications
- Microtubule and mitosis dynamics
- Developmental Biology and Gene Regulation
- Optical Imaging and Spectroscopy Techniques
- Healthcare cost, quality, practices
- Pancreatic function and diabetes
- Hernia repair and management
- Hypothalamic control of reproductive hormones
- Pluripotent Stem Cells Research
Institut de la Vision
2014-2023
Inserm
2014-2023
Sorbonne Université
2014-2023
Centre National de la Recherche Scientifique
2014-2023
Université Sorbonne Nouvelle
2020
Université Paris Cité
2014
Abstract Human induced pluripotent stem cells (hiPSCs) are potentially useful in regenerative therapies for retinal disease. For medical applications, therapeutic cells, such as pigmented epithelial (RPE) or photoreceptor precursors, must be generated under completely defined conditions. To this purpose, we have developed a two-step xeno-free/feeder-free (XF/FF) culture system to efficiently differentiate hiPSCs into cells. This simple method, relies only on adherent cultured chemically...
Clearing techniques have been developed to transparentize mouse brains, thereby preserving 3D structure, but their complexity has limited use. Here, we show that immunolabeling of axonal tracts followed by optical clearing with solvents (3DISCO) and light-sheet microscopy reveals brain connectivity in embryos postnatal brains. We the Robo3 receptor is selectively expressed medial habenula axons forming fasciculus retroflexus (FR) analyzed development this commissural tract mutants Slit/Robo...
Fertility in mammals is controlled by hypothalamic neurons that secrete gonadotropin-releasing hormone (GnRH). These differentiate the olfactory placodes during embryogenesis and migrate from nose to hypothalamus before birth. Information regarding this process humans sparse. Here, we adapted new tissue-clearing whole-mount immunohistochemical techniques entire human embryos/fetuses meticulously study system first trimester of gestation largest series fetuses examined date. Combining these...
Recent histopathological studies have shown that neurodegenerative processes in Alzheimer's and Parkinson's Disease develop along neuronal networks hallmarks could propagate trans-synaptically through pathways. The underlying molecular mechanisms are still unknown, investigations been impeded by the complexity of brain connectivity need for experimental models allowing a fine manipulation local microenvironment at subcellular level.In this study, we grown primary cortical mouse neurons...
The oocyte must grow and mature before fertilization, thanks to a close dialogue with the somatic cells that surround it. Part of this communication is through filopodia-like protrusions, called transzonal projections (TZPs), sent by membrane. To investigate contribution TZPs quality, we impaired their structure generating full knockout mouse TZP structural component myosin-X (MYO10). Using spinning disk super-resolution microscopy combined machine-learning approach phenotype morphology,...
DCC, a NETRIN-1 receptor, is considered as cell-autonomous regulator for midline guidance of many commissural populations in the central nervous system. The corticospinal tract (CST), principal motor pathway voluntary movements, crosses anatomic at pyramidal decussation. CST fails to cross Kanga mice expressing truncated DCC protein. Humans with heterozygous mutations have congenital mirror movements (CMM). As CMM has been associated, some cases, malformations decussation, might also be...
Abstract The ocular vasculature is critically involved in many blinding diseases and also a popular research model for the exploration of developmental pathological angiogenesis. development vessels complex, finely orchestrated sequence events, involving spatial temporal coordination hyaloid, choroidal retinal networks. Comprehensive studies tridimensional dynamics microvascular remodeling are limited by fact that preserving disposition vascular networks cumbersome using classical...
Abstract The cholinergic system plays a major anti-inflammatory role in many diseases through acetylcholine (Ach) release after vagus nerve stimulation. Osteoarthritis (OA) is associated with local low-grade inflammation, but the regulatory mechanisms are unclear. Local Ach could have activity since articular cells express receptors involved inflammatory responses. Using 3DISCO clearing protocol that allows whole-sample 3-dimensional (3D) analysis, we cleared human OA cartilage-subchondral...
Abstract In Bilaterians, commissural neurons project their axons across the midline of nervous system to target on opposite side. mammals, crossing at level hindbrain and spinal cord requires Robo3 receptor which is transiently expressed by all neurons. Unlike other Robo receptors, mammalian receptors do not bind Slit ligands promote crossing. Surprisingly, much known about distribution mechanism action in vertebrate species. Here, we have used whole‐mount immunostaining, tissue clearing...
In the vertebrate retina six types of neurons and one glial cell type are generated from multipotent retinal progenitor cells (RPCs) whose proliferation differentiation regulated by intrinsic extrinsic factors. RPCs proliferate undergoing interkinetic nuclear migration within neuroblastic layer, with their nuclei moving up down along apico‐basal axis. Moreover, they only differentiate therefore exit cycle at apical side layer. Sema6A its receptors PlexinA4 PlexinA2 control lamina...
Second messengers, including cAMP, cGMP and Ca
Recent histopathological studies have shown that neurodegenerative processes in Alzheimer's and Parkinson's Disease develop along neuronal networks hallmarks could propagate trans-synaptically through pathways. The underlying molecular mechanisms are still unknown, investigations been impeded by the complexity of brain connectivity need for experimental models allowing a fine manipulation local microenvironment at subcellular level. In this study, we grown primary cortical mouse neurons...
Summary The cerebellum, a primary center involved in the control of sensorimotor tasks, also contributes to higher cognitive functions including reward, emotion and social interaction. regulation these behaviors has been largely ascribed monoaminergic system limbic regions. However, contribution cerebellar dopamine signaling modulation remains unknown due lack precise characterization dopaminoceptive neurons. By combining cell type-specific transcriptomic histological analyses, 3D imaging...
Earlier data on liver development demonstrated that morphogenesis of the bile duct, portal mesenchyme and hepatic artery is interdependent, yet how this interdependency orchestrated remains unknown. Here, using 2D 3D imaging, we first describe mesenchymal cells become organised to form arteries. Next, examined intercellular signalling active during area found axon guidance genes are dynamically expressed in developing ducts mesenchyme. Using tissue-specific gene inactivation mice, show...
A bstract Second messengers, including cAMP, cGMP and Ca 2+ are often placed in an integrating position to combine the extracellular cues that orient growing axons developing brain. This view suggests axon repellents share same set of cellular messenger signals attractants evoke opposite changes. Investigating confinement these second messengers nanodomains, we instead demonstrate two repellent cues, ephrin-A5 Slit1, induce spatially segregated signals. These guidance molecules activate...
Summary The oocyte must grow and mature before fertilization, thanks to a close dialogue with the somatic cells which surround it. Part of this communication is through filopodialike protrusions, called transzonal projections (TZPs), sent by membrane. To investigate contribution TZPs quality, we impaired their structure generating full knockout mouse TZP structural component Myosin-X (MYO10). Using spinning disk super-resolution microscopy combined machine learning approach phenotype...