A5045825658- Ruminant Nutrition and Digestive Physiology
- Animal Nutrition and Physiology
- Effects of Environmental Stressors on Livestock
- Genetic and phenotypic traits in livestock
- Ultrasound in Clinical Applications
- Crop Yield and Soil Fertility
- Agriculture Sustainability and Environmental Impact
- Meat and Animal Product Quality
- Animal Behavior and Welfare Studies
- Rabbits: Nutrition, Reproduction, Health
- Animal health and immunology
- Reproductive Physiology in Livestock
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Traumatic Brain Injury and Neurovascular Disturbances
- Pancreatic function and diabetes
- Cerebral Venous Sinus Thrombosis
- Microbial infections and disease research
- Food composition and properties
- Hemodynamic Monitoring and Therapy
- Radiology practices and education
- Simulation-Based Education in Healthcare
- Agricultural Systems and Practices
- Central Venous Catheters and Hemodialysis
- Escherichia coli research studies
Kansas State University
2006-2024
Duke University Health System
2023-2024
Duke University
2017-2023
Duke University Hospital
2020-2023
Duke Medical Center
2020-2023
Arkansas Agricultural Experiment Station
2001-2022
Syngenta (United States)
2022
Tisch Hospital
2020
Tisch Cancer Institute
2020
United States Army
2019
Vaginal anti-HIV antibody responses may be beneficial, and possibly required, for vaccine-induced protection against HIV infection acquired through receptive vaginal intercourse. We have previously determined that intranasal immunization with a hybrid peptide cholera toxin induced IgA in BALB/c C57BL/6 mice. To determine if vaginal, gastric, or rectal boosting would enhance the induction of over those observed only, mice were intranasally immunized T1SP10MN(A) (days 0 14) boosted via route 7...
Background. Allogeneic skin transplantation remains a rigorous test of any immune intervention designed to prevent allograft rejection. To date, no single, clinically available immunosuppressant has been reported induce long-term primary survival in primates. We have previously shown that treatment with the humanized CD154-specific monoclonal antibody, 5C8 (hu5C8), induces renal nonhuman In this study, we evaluated efficacy hu5C8 preventing rejection rhesus monkeys. Methods. Ten monkeys were...
Abstract Anti-CD154 variably prolongs allograft survival in nonhuman primates. Rodent studies suggest that adding pretransplant donor-specific transfusion (DST) and/or rapamycin to anti-CD154 improves survival. The CD154-specific Ab IDEC-131 was tested alone and combination with for its ability inhibit rhesus MLRs. of the block endothelial activation also assessed. then DST prevent rejection full-thickness, MHC-mismatched skin allografts. Animals were monitored hyporesponsiveness by MLR...
Background: Up to 20% of all trauma patients admitted an intensive care unit die from their injuries. End-of-life decision making is a variable process that involves prognosis, predicted functional outcomes, personal beliefs, institutional resources, societal norms, and clinician experience. The goal this study was better understand end-of-life processes after major injury by comparing viewpoints various countries cultures. Methods: A clinician-based, 38-question international survey used...
Background. Antibodies and fusion proteins specific for CD80, CD86, CD154 have shown promise as agents capable of inducing donor-specific tolerance in rodents. These also been to be synergistic with one another many settings counter-adaptive immunity. In the nonhuman primate, monoclonal antibodies CD80 CD86 prolonged time rejection renal allografts but not resulted tolerance. A antibody has markedly survival kidney, islet, cardiac, skin allografts, again most animals eventually developed...
Six hundred sixty-five crossbred beef heifers initially weighing 225 kg were used in a completely randomized design to measure plasma glucose, lactate, and urea N concentrations at time of initial processing, determine the incidence apparent bovine respiratory disease (BRD) receiving cattle, evaluate effect BRD on subsequent cattle growth carcass characteristics. Heifers processed within 24 h arrival, processing included vaccination against common viral clostridial diseases, recording rectal...
The focused ultrasound examination has become increasingly recognized as a safe and valuable diagnostic tool for the bedside assessment of critically ill patient. We implemented dedicated on-site critical care ultrasonography curriculum with goal developing model teaching skills to pulmonary medicine fellows.The program was comprised blended didactic sessions in following topic domains: fundamentals; vascular access diagnosis; abdominal, thoracic, cardiac ultrasonography. Formal knowledge...
Five Holstein steers (235 kg of BW) fitted with ruminal, duodenal, and ileal cannulas were used in a 5 x Latin square design experiment to determine the effects supplemental fat source on site extent nutrient digestion ruminal fermentation. Treatments diets based steam-flaked corn containing no (control) or 4% (DM basis) as tallow, dried full-fat germ (corn germ), oil, flax oil. Fat supplementation decreased (P < 0.08) starch but increased 0.03) small intestinal percentage intake. Feeding...
We’ve established a nonhuman primate islet allotransplant model to address questions such as whether transplanting islets into the gut’s arterial system would more safely and effectively support long-term allograft survival compared with traditional portal vein approach. We reasoned that make up &lt;2% of pancreatic cell mass but consume an estimated 20% blood flow, suggesting advantage for site. Access is also easier safer than system. Pancreatectomized rhesus macaques were transplanted...
BACKGROUND Ultrasound (US) for the detection of pneumothorax shows excellent sensitivity in hands skilled providers. Artificial intelligence may facilitate movement US into prehospital setting. The large amount training data required conventional neural network methodologies has limited their use so far. METHODS A database was supplied by Defense Advanced Research Projects Agency 30 patients, 15 cases with and without. There were two videos per patient, which we allowed to choose one train...
The objective was to determine the effects of ad libitum-fed roughage-based diets or limit-fed high-energy on growth performance, behavior, health, and digestion in newly received growing cattle subsequent implications feedlot performance carcass characteristics. In experiment 1, 409 crossbred heifers (initial body weight [BW] = 279 ± 24 kg) 32 pens were used a randomized block design. Heifers fed one two dietary treatments: total mixed ration with 0.99 Mcal net energy for gain (NE
Performance and digestibility experiments were conducted to determine the influence of moisture flake density (FD) on feeding value steam-flaked corn (SFC). Dietary treatments consisted finishing diets that contained 78% (DM basis) SFC was tempered using 0, 6, or 12% processed either 360 (SF28) 310 (SF24) g/L. A 3 x 2 factorial arrangement used. In Exp. 1, 78 steers individually fed respective for 106 d. Moisture added during tempering tended (linear; P < 0.10) increase starch availability...
The objective of this project was to design an airplane with the ability fly all day and night using only solar power. scope limited by specifying a maximum gross weight 5 kg area 1.5 m 2 for combined wing plus horizontal stabilizer area. presented in paper, named Photon, achieved both these objectives. key features Photon are lack ailerons, cruise/climb battery configurations, custom propeller design. potential benefit in-flight adjustable pitch also investigated. designed use Sunpower...
The anti-CD154 antibody hu5C8 prevents acute allograft rejection and prolongs survival after withdrawal of therapy in nonhuman primates. This study describes the use as a rescue agent for developing hu5C8. Twelve rhesus monkeys that had received renal allografts under induction subsequently rejected were studied. Rescue with was analyzed based on histological character (acute versus chronic) whether conventional at time or induction. diagnosis response to function histology. Four associated...
A regimen combining sirolimus, tacrolimus, and daclizumab has recently been shown to provide adequate immunosuppression for allogeneic islet transplantation in humans, but remains unproven primarily vascularized allografts. We evaluated this renal allograft mismatched nonhuman primates. Dosages of sirolimus tacrolimus were adjusted trough levels 10–15 ng/mL 4–6 ng/mL, respectively. Treated monkeys (n = 5) had significantly prolonged survival, with a mean survival 36 days vs. 7 untreated...
Twelve ruminally cannulated Jersey steers (BW = 534 kg) were used in an incomplete Latin square design experiment with a 2 × factorial arrangement of treatments to determine the effects wet corn gluten feed (WCGF) and total DMI level on diet digestibility ruminal passage rate. Treatments consisted diets formulated contain (DM basis) steam-flaked corn, 20% coarsely ground alfalfa hay, either 0 or 40% WCGF offered once daily for ad libitum consumption limited 1.6% BW basis). Two consecutive...
One finishing trial and one digestibility were used to evaluate wet corn gluten feed (WCGF) alfalfa hay (AH) combinations in steam-flaked (SFC) diets. In Exp. 1, 631 crossbred heifers (initial BW = 284 +/- 7.9 kg) fed SFC-based diets containing of WCGF (25, 35, or 45% diet DM) AH (2 6% dietary a 2 x 3 factorial arrangement treatments. No interactions existed between for heifer performance. Increasing linearly decreased gain efficiency (P < 0.01), NEg concentration 0.05), 12th-rib fat...
Crossbred beef steers (n = 615) were used in a 152-d experiment to compare steam-flaked corn (SFC) diets containing 0, 30, or 60% wet gluten feed (WCGF). On d 114 118, ruminal and fecal samples collected from 180 analyzed for pH, VFA, total acid-resistant Escherichia coli coliforms. Acid resistance of E. coliform populations was determined by exposure the 1 h pH 2, 4, 7 citric acid/sodium phosphate buffers. Increasing levels WCGF linearly decreased VFA (P 0.01) 0.06), but increased...
Two experiments were conducted to evaluate dried full-fat corn germ (GERM) as a supplemental fat source in cattle finishing diets. In Exp. 1, 24 pens totaling 358 crossbred beef steers with an initial BW of 319 kg allowed ad libitum access diets containing dry-rolled corn, 35% wet gluten feed, and 0, 5, 10, or 15% GERM on DM basis. Increasing decreased (linear; P < 0.02) DMI increased (quadratic; ADG. Steers fed 10% had the greatest ADG G:F 0.05). The addition 0.05) thickness, KPH,...
Effects of corn processing and dietary inclusion wet gluten feed (WCGF) on growth performance digestibility were analyzed in 2 experiments. Dietary treatments arranged as a × factorial. Diets contained either whole-shelled (WSC) or dry-rolled (DRC), they 47% with no WCGF 29% 30% WCGF. In Exp. 1, 279 crossbred calves (230 kg) allocated to complete block design used measure digestion during 60-d receiving period. Corn did not affect (P ≥ 0.31) performance. Inclusion the diet increased final BW...