Two bacterial strains, KMM 227T and 231T, were isolated from seawater samples collected the north-western Pacific Ocean at a depth of 4000-5000 m characterized using polyphasic taxonomy. Both Gram-negative, psychrotolerant, heterotrophic, aerobic required NaCl for growth (0.6-15.0%). The temperature was 4-30 degrees C. strains rod-shaped, with single flagellum. However, strain 231T revealed long fimbrium. Cellular fatty acids detected in isolates predominantly odd-numbered iso-branched, 15...

Some marine and extremophilic microorganisms are capable of synthesizing sulfated polysaccharides with a unique structure. A number studies indicate significant biological properties individual polysaccharides, such as antiproliferative activity, which makes them promising area for further research. In this study, the capsular polysaccharide (CPS) was obtained from bacterium Cobetia marina KMM 1449, isolated sediment sample collected along shore Sea Japan. The CPS by saline solution,...

A Gram-negative aerobic, motile bacterium KMM 10153T was isolated from bottom sediment sampled the Sea of Japan at a depth 256 m, Russia. Strain grew in 0–12% NaCl temperatures ranging 4 to 42 °C and produced brown diffusible pigments. Based on 16S rRNA gene whole genome sequences analyses, novel affiliated with genus Oceanisphaera (phylum Pseudomonadota) showing highest sequence similarities 98.94% arctica KCTC 23013T, 98.15% donghaensis BL1T, similarity values <98% other validly...

Two novel strains, CB1-14T and CB2-10, were isolated from the marine polychaetes Chaetopterus cautus Sea of Japan. Phylogenetic analysis based on 16S rRNA sequences revealed that two strains belong to genus Vibrio, sharing 98.96% identity with Vibrio hangzhouensis CN 83T. MLSA using five protein-coding genes (ftsZ, gyrA, gyrB, mreB, rpoA) showed CB2-10 are closely related members Mediterranei clade, namely mediterranei CECT 621T, barjaei 3062T, thalassae 8203T, CGMCC 1.7062T, maritimus CAIM...

Two Gram-negative, non-fermentative, non-denitrifying, non-pigmented, rod-shaped bacteria that were motile by means of polar flagella, designated strains KMM 330 T and 331, isolated from a deep-sea sponge specimen subjected to polyphasic taxonomic study. The new isolates exhibited 16S rRNA gene sequence similarity 99·9 %, their mean level DNA–DNA relatedness was 82 %. Phylogenetic analysis based on sequences placed the within genus Pseudomonas as an independent deep clade. Strain shared...

Two marine, Gram-negative, aerobic, halophilic strains, designated KMM 3657 T and 3840 , were isolated found to be phylogenetically closely related each other, showing 96·6 % 16S rRNA gene sequence similarity. Both strains are members of the genus Marinobacter in γ - Proteobacteria (94·7–98·0 similarity). Strain lipolyticus SM19 related, with 98·0 The novel shared generic physiological chemotaxonomic properties species, but differed their temperature range for growth, inability grow 20 NaCl...

Four strains of Gram-negative, aerobic, psychrotolerant, non-motile, non-pigmented bacteria were isolated from coastal sea-ice and sediment samples. These displayed the general chemotaxonomic phenotypic features members genus Psychrobacter . 16S rRNA gene sequencing positioned three isolates KMM 3646 T , 3643 3645 isolate 3659 in two distinct lineages within displaying less than 98·5 % sequence similarity to type other species. Genomic distinctness was supported by differences growth...

An aerobic, Gram-negative bacterium, strain KMM 329(T), was isolated from a deep-sea sponge specimen the Philippine Sea and subjected to polyphasic taxonomic investigation. Comparative 16S rRNA gene sequence analysis showed that 329(T) clustered with species of genus Lysobacter. The highest level similarity (97.0 %) found respect Lysobacter concretionis KCTC 12205(T); lower values (96.4-95.2 were obtained other recognized species. value for DNA-DNA relatedness between L. 12205(T) 47 %....

A group of five Gram-negative, aerobic, halotolerant, non-pigmented bacteria isolated from shallow sediment samples and invertebrate specimens collected the Gulf Alaska Sea Japan was subjected to taxonomic study. On basis 16S rRNA gene sequence analysis, novel isolates were affiliated genus Cobetia , sharing highest similarity 99.3–99.9 % with marina DSM 4741 T . DNA–DNA hybridization experiments between among strains C. crustatorum JCM 15644 revealed that represent three separate...

A Gram-negative, aerobic, rod-shaped, non-motile, yellow-pigmented bacterium, KMM 9835T, was isolated from the sediment sample obtained Amur Bay of Sea Japan seashore, Russia. Phylogenetic analyses based on 16S rRNA gene and whole genome sequences positioned novel strain 9835T in genus Mariniflexile as a separate line sharing highest sequence similarities 96.6% 96.2% with soesokkakense RSSK-9T fucanivorans SW5T, respectively, similarity values <96% to other recognized species. The average...

Two novel psychrophilic, halophilic, Psychrobacter-like bacteria, strains KMM 225T and 277T, were isolated from sea water the internal tissues of an ascidian Polysyncraton sp. specimen, respectively, characterized using a polyphasic approach, which included phenotypic, genotypic, chemotaxonomic phylogenetic analyses. The marine isolates Gram-negative, aerobic, coccoid, oxidase- catalase-positive, non-pigmented, non-motile, psychrophilic halophilic they utilized restricted spectrum carbon...

Alteromonas-like strains KMM 241T and 642, isolated from marine invertebrate specimens, were investigated to clarify their taxonomic position. The novel isolates aerobic, Gram-negative, motile, slightly halophilic heterotrophic hydrolysed polysaccharides. They did not hydrolyse urea, gelatin or casein produced acid weakly carbohydrates. DNA G+C content ranged between 44·6 44·8 mol%. DNA–DNA similarity the two was 71 %. Comparison of 16S rRNA gene sequence strain revealed 94·5–94·8 %...

Two Pseudomonas-like yellow-orange-pigmented non-fluorescent denitrifying strains KMM 235 and 1447T were isolated from marine ascidian specimens investigated by a polyphasic approach to clarify their taxonomic status. On the basis of 16S rDNA gene sequence data new isolates clustered with Pseudomonas stutzeri species group similarities >98%. The results DNA-DNA hybridization biochemical characterization showed genetic phenotypic distinction between other validly described species. Strain was...

An aerobic, Gram-stain-negative, yellow-pigmented, non-motile, rod-shaped bacterium designated strain KMM 9005(T) was isolated from a sediment sample collected the Sea of Japan seashore. Comparative 16S rRNA gene sequence analysis showed that belonged to genus Luteimonas and most closely related cucumeris KCTC 23627(T) (96.5 % similarity) aquatica LMG 24212(T) (96.1 similarity). Strain characterized by presence thin fimbriae, major ubiquinone Q-8, predominance iso-C17 : 1 followed iso-C16 0,...

A Gram-negative, oxidase- and catalase-positive, rod-shaped bacterium, designated strain KMM 3655 T , was isolated from a coastal marine sediment sample. The novel bacterium required sodium ions for growth grew between 0·5 5 % NaCl at 4–37 °C, but not 40 °C. It reduced nitrate, formed acids glucose under aerobic anaerobic conditions, utilized limited spectrum of organic substrates did produce gelatinase, caseinase, amylase or chitinase. major isoprenoid quinone Q8. Polar lipids consisted...

The phenotypic, genomic and phylogenetic characteristics of four aerobic, Gram-negative, non-fermentative, motile, non-pigmented, agarolytic Pseudoalteromonas-like bacteria, isolated from marine environments, have been investigated. These bacteria share DNA–DNA similarities above 86 %. Comparative 16S rDNA sequence analysis strain KMM 255T revealed its membership the genus Pseudoalteromonas; it shares 99·9 % similarity with Pseudoalteromonas distincta, elyakovii, atlantica espejiana....

An aerobic, Gram-negative, yellow-pigmented, non-motile bacterium, designated strain KMM 3882 T , was isolated from a marine bivalve ( Anadara broughtoni ) collected Peter the Great Bay, Sea of Japan, and subjected to phenotypic phylogenetic analyses. Strain found exert remarkable inhibitory activity against number Gram-positive micro-organisms. Phylogenetic analysis based on 16S rRNA gene sequences placed within genus Sphingomonas as an independent lineage adjacent dokdonensis DS-4 panni...

A Gram-negative, aerobic, greyish–yellowish-pigmented, stenohaline, rod-shaped, non-motile bacterium, strain KMM 3900 T , was isolated from a coastal seawater sample collected the Sea of Japan. Based on phylogenetic analysis, positioned within Gammaproteobacteria separate branch adjacent to members genera Reinekea and Kangiella sharing less than 88 % 16S rRNA gene sequence similarity with all recognized species . The major isoprenoid quinone Q-8. Polar lipids consisted phosphatidylcholine,...

An aerobic, Gram-negative, motile, non-pigmented bacterium, strain KMM 3042(T), isolated from a deep-sea brittle star in the Fiji Sea, was subjected to polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed marine Mn(II)-oxidizing isolate S185-2B as closest neighbour of 3042(T) (99.9 % sequence similarity). The two strains formed distinct lineage within genus Pseudomonas adjacent members borbori cluster, sharing highest similarity 97.4 and 97.0 %,...