Filamentous fungi produce a great variety of enzymes, and research on their biotechnological potential has recently intensified. The objective this work was to identify, at the species level, using DNA barcoding, 46 fungal isolates obtained from maize grains with rot symptoms. We also analyzed production extracellular amylases, cellulases, proteases lipases 33 those isolates. enzymatic activities were evaluated by formation clear halo or white precipitate around colonies in defined substrate...

The human <i>asparagine synthetase</i>(<i>AS</i>) gene is transcriptionally regulated by amino acid deprivation (amino response, AAR) and the endoplasmic reticulum stress response (ERSR), also known as unfolded protein pathway. results reported here document novel observation that induction of <i>AS</i> AAR ERSR pathways occurs via same set genomic elements. Data supporting this conclusion include transient transfection AS promoter/reporter constructs illustrate transcriptional control...

Nutrients such as glucose regulate the expression of genes that are involved in plasma membrane transport, metabolic functions, and protein trafficking endoplasmic reticulum. Depletion nutrients results cellular stress, which evokes adaptive protective responses, one is induction heme oxygenase-1 (HO-1), a 32-kDa reticulum enzyme catalyzes rate-limiting step degradation. Incubation HepG2 human hepatoma cells glucose-free medium resulted an increased HO-1 mRNA content, reaching maximum...

Fusarium verticillioides and subglutinans are important fungal pathogens of maize other cereals worldwide. In this study, we developed PCR-based protocols for the identification these targeting gaoB gene, which codes galactose oxidase. The designed primers recognized isolates F. that were obtained from seeds several producing regions Brazil but did not recognize spp. or genera either collections isolated seeds. A multiplex PCR protocol was established to simultaneously detect genomic DNA...

Abstract This study describes for the first time purification and characterization of a glucoamylase from Aspergillus wentii (strain PG18), species genus Cremei section. Maximum enzyme production (∼3.5 U/ml) was obtained in submerged culture (72 h) with starch as carbon source, at 25°C, orbital agitation (100 rpm). The purified one‐step molecular exclusion chromatography. 86 kDa hydrolyzed zymogram had activity against p ‐nitrophenyl α‐ d ‐glucopyranoside. optimal pH temperature were 5.0...

The productivity of wheat and corn crops depends on climatic conditions resistance against phytopathogenic fungi such as those the genus Fusarium. Some species this produce zearalenone (ZEA), a mycotoxin with hyperestrogenic effects. objective study was to investigate presence ZEA in samples cracked (n = 109), popcorn 51) grits 50) commercialized State Paraná, Brazil. Commercial each crop were collected between September 2007 June 2008 analyzed by thin-layer chromatography. method used for...

Transcription of the asparagine synthetase (AS) gene is induced by amino acid deprivation. The present data illustrate that this also under transcriptional control carbohydrate availability. Incubation human HepG2 hepatoma cells in glucose-free medium resulted an increased AS mRNA content, reaching a maximum about 14-fold over after approx. 12 h. Extracellular glucose caused repression content concentration-dependent manner, with k1/2 (concentration causing half-maximal repression) 1 mM....

The objective of this work was to compare the polymerase chain reaction (PCR) using lesion scrapping with other conventional techniques for diagnosis American tegumentary leishmaniasis (ATL). For this, patients cutaneous lesions suspected be ATL were studied. DNA amplified MP1L/MP3H primers. From 156 studied patients, 79 (50.6%) presented positive parasite direct search (PD), 81 (51.9%) had Montenegro skin test (MST), and 90 (57.7%) PD and/or MST positive. PCR in all positive-PD (100%...

Aflatoxins are mutagenic, carcinogenic, and teratogenic mycotoxins. The objective of this work was to study the presence aflatoxigenic Aspergillus in commercial Bulgur wheat city Maringá, Paraná, Brazil. Thirty samples wheat, acquired period August 2011 January 2012, were evaluated. enumeration analysis showed that had up 273.3 CFU molds 133.3 per gram wheat. Forty-two monosporic isolates obtained identified as flavus. analyzed regarding their potential by culture coconut milk agar;...

The presence of mycotoxins or related fungi in animal feed is a major problem for and human health. Silage concentrated samples were collected from 21 dairy farms the Western part Paraná state Southern Brazil. Water activity pH all measured, each sample was analyzed to check aflatoxigenic Aspergillus. observed be lower samples. silage samples, indicating fermentation processes. Two four contaminated with Aspergillus spp. Seven isolates obtained their potential produce aflatoxins evaluated....

American tegumentary leishmaniasis (ATL) was studied in 143 dogs a rural area the county of Mariluz, northwestern Paraná State, Brazil, using direct parasite search, indirect immunofluorescence (IIF), and polymerase chain reaction (PCR). Thirty-nine (27.3%) presented lesions suggestive disease, 5 (12.8%) which were positive search PCR (lesion), these 5, 4 also by IIF. Of 34 with negative 12 (35.3%) had PCR- lesions, these, IIF-positive. One hundred four no but 17/101 (16.8%) blood 10/38...

The main objective of this work was to develop a PCR protocol for the identification Fusarium graminearum, based on pair primers targeted segment 3' coding region gaoA gene that codes enzyme galactose oxidase (GO). This has low homology with same GO genes from other fungi. Genomic DNA 17 strains spp. isolated diseased cereals, several species, and fungi genera analyzed in assay using primer set. were also production submerse fermentation new formulated liquid medium. All morphologically...

Some microorganisms can produce cyclodextrin glycosyltransferases, which degrades starch by catalyzing cyclization and giving rise to cyclodextrin. Thus, fully degrade starch, also synthesize cyclodextrinases, hydrolyze cyclodextrins. In this work, a truncated gene, without the signal peptide coding sequence, encoding cyclodextrinase from Massilia timonae was PCR amplified, cloned, expressed in E. coli. The histidine-tagged recombinant enzyme purified immobilized metal ion affinity...

Extra-cellular production of a novel galactose oxidase from Fusarium acuminatum using submerged fermentation was studied. Glucose (1.0% w/v) used as the sole carbon source. Maximum (approximately 4.0 U/ml) obtained when carried out at 25 degrees C, with orbital shaking (100 rpm) and an initial medium pH 7.0, for 96 h, 2% (v/v) inoculum made homogenized four-day-old liquid culture, in presence copper, manganese, magnesium. The enzyme purified by one-step affinity chromatography, recovery 42%...