A5063916332- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Wheat and Barley Genetics and Pathology
- Phytase and its Applications
- Mycotoxins in Agriculture and Food
- Transgenic Plants and Applications
- Microbial Metabolism and Applications
- Bone and Dental Protein Studies
- Cancer Research and Treatments
- Microbial Metabolites in Food Biotechnology
- Milk Quality and Mastitis in Dairy Cows
- Protein Hydrolysis and Bioactive Peptides
- Plant Disease Resistance and Genetics
- Enzyme-mediated dye degradation
Universidade Estadual de Maringá
2015-2023
Filamentous fungi produce a great variety of enzymes, and research on their biotechnological potential has recently intensified. The objective this work was to identify, at the species level, using DNA barcoding, 46 fungal isolates obtained from maize grains with rot symptoms. We also analyzed production extracellular amylases, cellulases, proteases lipases 33 those isolates. enzymatic activities were evaluated by formation clear halo or white precipitate around colonies in defined substrate...
Abstract This study describes for the first time purification and characterization of a glucoamylase from Aspergillus wentii (strain PG18), species genus Cremei section. Maximum enzyme production (∼3.5 U/ml) was obtained in submerged culture (72 h) with starch as carbon source, at 25°C, orbital agitation (100 rpm). The purified one‐step molecular exclusion chromatography. 86 kDa hydrolyzed zymogram had activity against p ‐nitrophenyl α‐ d ‐glucopyranoside. optimal pH temperature were 5.0...
Aflatoxins are mutagenic, carcinogenic, and teratogenic mycotoxins. The objective of this work was to study the presence aflatoxigenic Aspergillus in commercial Bulgur wheat city Maringá, Paraná, Brazil. Thirty samples wheat, acquired period August 2011 January 2012, were evaluated. enumeration analysis showed that had up 273.3 CFU molds 133.3 per gram wheat. Forty-two monosporic isolates obtained identified as flavus. analyzed regarding their potential by culture coconut milk agar;...
The presence of mycotoxins or related fungi in animal feed is a major problem for and human health. Silage concentrated samples were collected from 21 dairy farms the Western part Paraná state Southern Brazil. Water activity pH all measured, each sample was analyzed to check aflatoxigenic Aspergillus. observed be lower samples. silage samples, indicating fermentation processes. Two four contaminated with Aspergillus spp. Seven isolates obtained their potential produce aflatoxins evaluated....
Some microorganisms can produce cyclodextrin glycosyltransferases, which degrades starch by catalyzing cyclization and giving rise to cyclodextrin. Thus, fully degrade starch, also synthesize cyclodextrinases, hydrolyze cyclodextrins. In this work, a truncated gene, without the signal peptide coding sequence, encoding cyclodextrinase from Massilia timonae was PCR amplified, cloned, expressed in E. coli. The histidine-tagged recombinant enzyme purified immobilized metal ion affinity...
Brazil is the third world largest maize producing country, and Paraná state second producer of this essential crop in country.The bacterial microbiota cereal grains depends on environment where they were grown, handled, processed -and it can influence plant growth food safety.The industrial enzymes market rapidly increasing around globe, new microorganisms are demand.Hydrolases correspond to 75% all enzymes.Considering dearth information about that might produce hydrolases for degrading...
Starch processing industries use amylases, accounting for approximately 30% of the world’s enzyme market. Previously, an amylase-producing strain Epicoccum nigrum was isolated from maize grains. Although E. amylase production is already reported in literature, no published data on optimization or characterization produced exists. The objectives this work were to improve by PG 16 and purify characterize enzyme. best conditions submerged culture were: inoculum 4% (v v-1) a five-days-old...