A5021321148- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- Cancer-related Molecular Pathways
- Prostate Cancer Treatment and Research
- Epigenetics and DNA Methylation
- Signaling Pathways in Disease
- Cancer-related gene regulation
- Biochemical and Molecular Research
- Cancer, Hypoxia, and Metabolism
- Ferroptosis and cancer prognosis
- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
Temple University
2022-2024
Center for Personalized Cancer Treatment
2024
Abstract The Ser/Thr protein phosphatase 2 A (PP2A) regulates the dephosphorylation of many phosphoproteins. Substrate recognition are mediated by B regulatory subunits. Here, we report identification a substrate conserved motif [RK]-V-x-x-[VI]-R in FAM122A, an inhibitor B55α/PP2A. This is necessary for FAM122A binding to B55α, and computational structure prediction suggests motif, which helical, blocks docking same site. In this model, also spatially constrains access occluding catalytic...
The Ser/Thr protein phosphatase 2A (PP2A) is a highly conserved collection of heterotrimeric holoenzymes responsible for the dephosphorylation many regulated phosphoproteins. Substrate recognition and integration regulatory cues are mediated by B subunits that complexed to catalytic subunit (C) scaffold (A). PP2A/B55 substrate recruitment was thought be charge-charge interactions between surface B55α its substrates. Challenging this view, we recently discovered SLiM [ RK ]- V -x-x-[ VI R in...
<title>Abstract</title> Prostate cancer (PCa) is the most common diagnosed in men worldwide and second leading cause of cancer-related deaths US males 2022. also represents highest mortality disparity between non-Hispanic blacks whites. However, there a relatively small number prostate normal cell lines compared to other cancers. To identify molecular basis PCa progression, it important have epithelial (PrEC) as karyotypically possible. Our lab recently developed novel methodology for rapid...
Prostate cancer (PCa) is the most common diagnosed in men worldwide and was second leading cause of cancer-related deaths US males 2022. also represents highest mortality disparity between non-Hispanic blacks whites. However, there a relatively small number prostate normal cell lines compared to other cancers. To identify molecular basis PCa progression, it important have epithelial (PrEC) as karyotypically possible. Our lab recently developed novel methodology for rapid efficient...
Serine/threonine protein phosphatase 2 (PP2A) forms heterotrimeric holoenzymes, where a scaffold subunit bridges the PP2A catalytic to B regulatory subunit, e.g., B55α. The PP2A/B55α holoenzyme plays key roles in signaling and cell-cycle control targeting multiple substrates. Here, we describe semiquantitative approaches determine substrate specificity. Parts I II detail assess PP2A/B55α-mediated dephosphorylation of immobilized peptide variants. III IV methods PP2A/B55α-substrate-binding...