A5070697670- Cancer-related gene regulation
- Peptidase Inhibition and Analysis
- Bacterial biofilms and quorum sensing
- Cancer, Hypoxia, and Metabolism
- Probiotics and Fermented Foods
- CRISPR and Genetic Engineering
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Epigenetics and DNA Methylation
- Gut microbiota and health
- Chemical Synthesis and Analysis
- Bacterial Genetics and Biotechnology
- Identification and Quantification in Food
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Plant Pathogenic Bacteria Studies
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Biochemical and Structural Characterization
- Cancer Research and Treatments
- Amino Acid Enzymes and Metabolism
- Milk Quality and Mastitis in Dairy Cows
- Organoboron and organosilicon chemistry
University of Minnesota
2020-2025
Twin Cities Orthopedics
2024
Colorado State University
2020
Abstract N ‐Methylation of the peptide backbone confers pharmacologically beneficial characteristics to peptides that include greater membrane permeability and resistance proteolytic degradation. The borosin family ribosomally synthesized post‐translationally modified offer a post‐translational route install amide α‐ ‐methylations. Previous work has elucidated substrate scope engineering potential two examples type I borosins, which feature autocatalytic precursors encode ‐methyltransferases...
Abstract Peptide backbone α- N -methylations change the physicochemical properties of amide bonds to provide structural constraints and other favorable characteristics including biological membrane permeability peptides. Borosin natural product pathways are only known ribosomally encoded posttranslationally modified peptides (RiPPs) incorporate on translated Here we report discovery type IV borosin (termed ‘split borosins’), featuring an iteratively acting -methyltransferase separate...
There is a need for standardized, efficient, and practical sampling methods to support large population-based studies of the internal external epithelial microbiomes bovine udder. The primary objective this study was evaluate different devices isolation microbial DNA originating from teat epithelium. Secondary objectives were survey compare diversity canal using amplicon shotgun metagenomic sequencing approaches. To address these objectives, we enrolled convenience sample 24 Holstein dairy...
Ground beef can be a reservoir for variety of bacteria, including spoilage organisms, and pathogenic foodborne bacteria. These bacteria exhibit antimicrobial resistance (AMR) which is public health concern if in pathogens leads to treatment failure humans. Culture-dependent techniques are commonly used study individual bacterial species, but these unable describe the whole community microbial species (microbiome) profile AMR genes they carry (resistome), critical getting holistic perspective...
Abstract. In studies using bromodeoxyuridine (BrdUrd) and/or iododeoxyuridine (IdUrd) to label S phase cells in cancer patients, several unique observations were made regarding DNA replication sites and the organization of newly synthesized post‐mitotic cells. While majority tumour specimens removed at end infusions demonstrated concentration around nuclear membrane, biopsies obtained leukaemic patients 1 week later distinct patterns labelling. For example, one, two or all lobes granulocytes...
Through a combination of in vitro structure–activity assays, crystallography, and vivo knockout experiments, we have identified structurally characterized the first bacterial borosin natural product.
Bacterial resistance to antibiotics is a rapidly increasing threat human health. New strategies combat resistant organisms are desperately needed. One potential avenue targeting two-component systems, which the main bacterial signal transduction pathways used regulate development, metabolism, virulence, and antibiotic resistance. These systems consist of homodimeric membrane-bound sensor histidine kinase, cognate effector, response regulator. The high sequence conservation in catalytic...
Transformation, the uptake of DNA directly from environment, is a major driver gene flow in microbial populations. In bacteria, requires nuclease that processes dsDNA to ssDNA, which subsequently transferred into cell and incorporated genome. However, process archaea still unknown. Previously, we cataloged genes essential natural transformation Methanococcus maripaludis , but few homologs bacterial transformation-associated were identified. Here, characterize one gene, MMJJ_16440 (named here...
ABSTRACT Transformation, the uptake of DNA directly from environment, is a major driver gene flow in microbial populations. In bacteria, requires nuclease that processes dsDNA to ssDNA, which subsequently transferred into cell and incorporated genome. However, process archaea still unknown. Previously, we cataloged genes essential natural transformation Methanococcus maripaludis , but few homologs bacterial transformation‐associated were identified. Here, characterize one gene, MMJJ_16440...
Abstract Bacterial resistance to antibiotics is a rapidly increasing threat human health. New strategies combat resistant organisms are desperately needed. One potential avenue targeting two-component systems, which the main bacterial signal transduction pathways used regulate development, metabolism, virulence, and antibiotic resistance. These systems consist of homodimeric membrane-bound sensor histidine kinase, cognate effector, response regulator. The high sequence conservation in...