We investigated the pathophysiology of structurally based corporeal veno-occlusive dysfunction.We prospectively evaluated 24 impotent patients (mean age plus or minus standard error 46 +/- 3 years) who had exposure to vascular risk factors and/or disorders inducing diffuse trabecular structure alterations and underwent penile prosthesis insertion. Preoperative indexes function (flow maintain, venous outflow resistance pressure decay measurements using repeat dosing pharmacocavernosometry)...

In Brief Twenty-eight patients with 41 full-thickness decubitus ulcers were randomized to compare the Vacuum-Assisted Closure device (VAC) Healthpoint System (HP) of wound gel products in promoting ulcer healing. A total 22 35 completed 6-week trial treatment, during which time 2 (10%) VAC group (N =20) and (13%) HP = 15) healed completely. The mean percent reduction volume was 42.1% 51.8% (p 0.46). number PMNs lymphocytes per high-power field decreased increased 0.13, p 0.41 respectively)....

To investigate the role of cholinergic neurotransmission in erection, human penile corpus cavernosum tissue was studied. Electrical stimulation strips suspended an organ chamber induced contractions and relaxations that were blocked by tetrodotoxin. The also bretylium prazosin. Norepinephrine phenylephrine contracted isolated this response prevented stimulation-induced enhanced physostigmine partially atropine. effect atropine, but not physostigmine, bretylium. Corporal with norepinephrine...

microRNA (miRNA) are short, noncoding RNA that negatively regulate gene expression and may play a causal role in invasive breast cancer. Since many genetic aberrations of disease detectable early stages, we hypothesized miRNA dysregulation the predicted changes might also be found neoplasias. Expression profiling 365 by real-time quantitative polymerase chain reaction assay was combined with laser capture microdissection to obtain an epithelium-specific signature normal epithelium from...

The localization, synthesis, and activity of endothelin the receptor types mediating its effects in penile corpus cavernosum were investigated whole tissue cultured cells derived from this tissue. With immunocytochemistry, utilizing an antiendothelin 1 (ET-1) monoclonal antibody, endothelin-like immunoreactivity was localized intensely endothelium to a lesser degree trabecular smooth muscle. Human endothelial culture expressed preproendothelin mRNA, as determined by Northern blot analysis....

We hypothesised that gene expression in histologically normal (HN) epithelium (NlEpi) would differ between breast cancer patients and usual-risk controls undergoing reduction mammoplasty (RM), NlEpi from cancer-free prophylactic mastectomy (PM) samples high-risk women resemble HN expression. analysed 73 microdissected frozen tissue. In 42 samples, we used microarrays to compare 18 RM age-matched (9 oestrogen receptor (ER)+, 9 ER−) 6 PM patients. Data were using a Bayesian approach (BADGE),...

In the United States, radical retropubic prostatectomy for adenocarcinoma usually includes a staging pelvic lymphadenectomy. If frozen section analysis of lymph nodes fails to reveal any evidence metastases, prostate is removed. We have previously noted that as many 56% patients undergoing demonstrate rising serum prostate-specific antigen (PSA) levels by 4 years postoperatively. This report was designed determine whether micrometastases undetectable conventional pathologic methods: could...

Abstract Purpose: Previously, we found that gene expression in histologically normal breast epithelium (NlEpi) from women at high cancer risk can resemble NlEpi cancer-containing breasts. Therefore, hypothesized characteristic of a subtype might be seen breasts containing subtype. Experimental Design: We examined 46 cases microdissected untreated undergoing surgery. From 30 age-matched [15 estrogen receptor (ER)+, 15 ER−] used Affymetryix U133A arrays. 16 independent (9 ER+, 7 ER−),...

No AccessJournal of UrologyClinical Urology: Original Article1 Feb 1995Extensive Corporeal Fibrosis After Penile Irradiation Simon J. Hall, Guido Basile, Eduardo B. Bertero, Antonio de las Morenas, and Irwin. Goldstein HallSimon Hall , BasileGuido Basile BerteroEduardo Bertero MorenasAntonio Morenas GoldsteinIrwin. View All Author Informationhttps://doi.org/10.1097/00005392-199502000-00023AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints...

Abstract Background CDKN1C (also known as p57 KIP2 ) is a cyclin-dependent kinase inhibitor previously implicated in several types of human cancer. Its family members (CDKN1A/p21 CIP1 and B/p27 KIP1 have been breast cancer, but information about CDKN1C's role limited. We hypothesized that decreased may be involved carcinogenesis vivo . Methods determined rates allele imbalance or loss heterozygosity (AI/LOH) , using an intronic polymorphism, the surrounding 11p15.5 region 82 cancers....

No AccessJournal of UrologyClinical Urology: Original Article1 Apr 1995Systemic Sclerosis and Impotence: A Clinicopathological Correlation Ajay Nehra, Simon J. Hall, Guido Basile, Eduardo B. Bertero, Robert Moreland, Paul Toselli, Antonio Morenas De Las, Irwin Goldstein NehraAjay Nehra , HallSimon Hall BasileGuido Basile BerteroEduardo Bertero MorelandRobert Moreland ToselliPaul Toselli LasAntonio Las GoldsteinIrwin View All Author...

The technique of fine-needle aspiration (FNA) biopsy the thyroid is important to evaluate malignancy in nodules. Eighty-five percent FNA procedures lead sufficient cellular material for diagnosis. With more cells aspirated, chance sufficiency diagnosis increases. Large-bore needles being aspirated but bloodier specimens that may interfere with cytologic interpretation. Smallbore result too few We conducted a randomized prospective study contrasting 21-gauge and 25-gauge evaluation 50...

The synthesis and localization of endothelin-1 were studied in human rabbit bladder. In addition, the effects on smooth muscle tone cholinergic neurotransmission investigated Endothelin-like immunoreactivity was localized transitional epithelium, serosal mesothelium, vascular endothelium; bladder (non-vascular) that blood vessels; fibroblasts. With situ hybridization, transcripts endothelin messenger ribonucleic acid (mRNA) with same cellular distribution as endothelin-like immunoreactivity,...

Abstract It remains unclear whether hyperplastic breast lesions, especially with atypia, are cancer precursors or markers of increased risk. Quantified comparisons genomic alterations in coexisting lesions could address this question. Therefore, we examined allele imbalance (AI), also known as loss heterozygosity (LOH), at 20 microsatellite on nine chromosome arms, DNA from 106 samples microdissected 17 randomly selected cancer‐containing specimens: 13 simple (DH) and 45 atypical ductal...

The cytologic differential diagnosis of colloid nodule (CN) and the follicular variant papillary carcinoma (FVPC) is difficult with common morphologic features. To assess utility 18 morphometric parameters in these thyroid lesions we evaluated 31 FNA samples that had histologic confirmation diagnoses. These cases included 15 CN, 8 FVPC, usual (UVPC) for reference values. For analysis used an Optimas 4.0 image system. Comparing CN group UVPC revealed eight statistically significant...

Laminin is a major component of basement membranes. We previously reported that the globular region laminin B chain(s) and cross A chain play an active role in mouse lung branching morphogenesis. In this study, basic morphogenic cell behaviors modulated by were analyzed order to elucidate how glycoprotein promotes development. Cocultures epithelial mesenchymal cells from fetal lungs used determine effect site-specific monoclonal antibodies (AL-1, AL-2, AL-3, AL-4, AL-5) on adhesion,...

Approximately 10% of women with breast cancer develop a second tumor, either new primary or recurrence. Differentiating between these entities using standard clinical and pathologic criteria remains challenging. Ambiguous cases arise, misclassifications may occur. We investigated whether quantitative DNA fingerprinting, based on allele imbalance (AI) loss heterozygosity (LOH), could evaluate clonality distinguish from recurrence.We developed scoring system the AI/LOH fingerprints 20...

One model of breast tumorigenesis postulates a sequential evolution from normal to proliferative epithelium and eventually neoplasia, but genetic data support this progression have been limited. We wished determine whether atypical hyperplasia (AH), lesion conventionally classified treated as benign, associated with an increased risk developing carcinoma, might show evidence abnormalities. Using the polymerase chain reaction (PCR), we examined DNA extracted 12 separate AH lesions, six cancer...

Normal-appearing breast epithelium can contain genetic abnormalities, including allele imbalance (AI), also referred to as loss of heterozygosity. Whether abnormalities are associated with cancer or risk is unknown.We performed a miniallelotype, using 20 microsatellites, on each 460 histologically normal, microdissected terminal ducto-lobular units (TDLUs) from three groups women: sporadic patients (SP; n = 18), BRCA1 gene mutation carriers (BRCA1; 16), and controls undergoing reduction...