Diabetic wound is one of the common complications in diabetic patients, which exhibits chronic, hard-to-heal characteristics. The healing process wounds impaired by several factors, including excessive oxidative stress, blocked angiogenesis, and bacterial infection. therapeutic effects traditional microneedle patches remain not satisfactory, due to their difficulty simultaneously targeting multiple targets treat wounds. As such, there an urgent need develop a multifunctional (MN) patch for...

Chronic wounds caused by severe trauma remain a serious challenge for clinical treatment. In this study, we developed novel angiogenic 3D-bioprinted peptide patch to improve skin wound healing. The technology can fabricate individual patches according the shape characteristics of damaged tissue. Gelatin methacryloyl (GelMA) and hyaluronic acid (HAMA) have excellent biocompatibility biodegradability, were used as biomaterial produce bioprinted patches. pro-angiogenic QHREDGS was covalently...

Palmitic acid (PA) is the most common saturated long-chain fatty in food that causes cell apoptosis. However, little known about molecular mechanisms of PA toxicity. In this study, we explore effects on proliferation and apoptosis human osteoblast-like Saos-2 cells uncover signaling pathways involved process. Our study showed endoplasmic reticulum (ER) stress autophagy are PA-induced We found inhibited viability a dose- time-dependent manner. At same time, induced expression ER marker genes...

Palmitic acid (PA) is the most common saturated long-chain fatty that causes damage to heart muscle cells. However, molecular mechanism of PA toxicity in myocardial cells not fully understood. In present study, we explored effects on proliferation and apoptosis H9c2 cardiomyocytes, uncovered signaling pathways involved toxicity. Our study revealed induction both oxidative endoplasmic reticulum (ER) stresses exacerbation PA-treated Inhibition stress by N-acetylcysteine (NAC) reduced decreased...

The role of the gut microbiota in glucocorticoid (GC)-induced glycolipid metabolism disorder remains unclear. In our study, dysbiosis characterized by an increased abundance Proteobacteria / Parasuttrerella and a decreased Lachnospiraceae_NK4A136_group was observed mice with GC-induced disorder. Some bacteria were shared previous study patients endogenous hypercortisolism mouse model used study. Furthermore, depletion fecal transplantation control could alleviate Plasma acetic acid,...

Pancreatic β-cell damage is a critical pathological mechanism in the progression of obese type 2 diabetes mellitus (T2DM). However, exact underlying remains unclear. We established an T2DM mouse model via high-fat diet feeding. The protein expression profiles pancreatic tissues from normal and mice were analyzed, revealing that nuclear factor activated T cells 5 (NFAT5) ferroptosis are potential mediators mechanisms mice. In vitro, high glucose palmitate treatment resulted increased NFAT5...

Diabetic cardiomyopathy is mediated by multiple molecular mechanisms including endoplasmic reticulum (ER) stress. Curcumin, a phenolic compound, has cytoprotective properties, but its potential protective action against diabetic and the related are not fully elucidated. In this study, we evaluated effects of curcumin on cell viability apoptosis in palmitic acid (PA)-treated H9C2 cardiomyocytes investigated signaling pathways involved. Treatment with PA reduced viability, induced apoptosis,...

Highlights•FGF-21 treatment restored endothelial cell proliferation, migration, and tube-forming ability in vitro, which had been impaired due to hyperglycemia damage.•In both vivo vitro experiments, FGF-21 attenuated the inflammatory response induced by high glucose wounds skin cells.•The wound-healing angiogensis-promoting effects of were comparable those VEGF diabetic wounds.AbstractBackgroundWound healing is a complex biological process that can be individuals with diabetes. Diabetic are...

We aimed to determine the effects of curcumin on palmitic acid- (PA-) induced human osteoblast-like Saos-2 cell apoptosis and explore potential molecular mechanisms in vitro level.Saos-2 were cultured with PA or without curcumin, N-acetylcysteine (NAC, anti-oxidant), 3-methyladenine (3-MA, autophagy inhibitor) AY-22989 (autophagy agonist) H2O2. Then, alone combined viability, apoptosis, oxidative stress, detected by CCK-8, flow cytometry assay western blot.We found that was induced, stress...

Endoplasmic reticulum (ER) stress and autophagy are involved in myocardial ischemia‑reperfusion (I/R) injury; however, their roles this type of injury remain unclear. The present study investigated the ER autophagy, underlying mechanisms, H9c2 cells during hypoxia/reoxygenation (H/R) injury. Cell viability was detected by CCK‑8 assay. flux monitored with mCherry‑GFP‑LC3‑adenovirus transfection. expression levels autophagy‑related proteins stress‑related were measured western blotting....

Insulin receptor substrate 2 (IRS2) is a component of the insulin/insulin-like growth factor 1 (IGF1) signaling cascade, which plays an important role in mouse hypothalamic and ovarian functions. The present study was conducted to investigate IRS2 steroidogenesis, apoptosis, cell cycle proliferation granulosa cells (GCs). Flow cytometry CCK8 assay showed that knockdown inhibited proliferation, reduced viability, increased apoptosis GCs. also revealed expression Cyclin A1, B1 Bcl2...

This study investigated the role of natriuretic peptide receptor 2 (NPR2) on cell proliferation and testosterone secretion in mouse Leydig cells.Mouse testis different postnatal stages was isolated to detect expression C-type (CNP) its NPR2 by quantitative reverse transcription polymerase chain reaction (RT-qPCR). cells from were cultured treated with shNPR2 lentiviruses or CNP. And then cyclic guanosine monophosphate production, secretion, proliferation, cycle apoptosis analyzed ELISA,...

KLF11 regulates insulin gene expression through binding to the promoter and has been reported as a causative for maturity-onset diabetes of young 7 (MODY7). Here, we report novel variant associated with three-generation family early childhood-onset explore its clinical functional characteristics. The three-generational pedigree contains five patients affected by diabetes. pathogenic identified whole-exome sequencing was further confirmed Sanger verification. Luciferase reporter assays...

<title>Abstract</title> Objective The effect of lifestyle intervention on the reduction fat in specific body areas for individuals with abdominal obesity has not been thoroughly studied. In this study, we evaluate if can uniquely influence various storage and to explore relationships between loss locations health results. Methods prospective cohort study conducted at a single center, 39 participated from October 18, 2022, April 20, 2023. Magnetic resonance imaging was used measure...